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Marine microbial agent and preparation method thereof

A technology of marine microorganisms and bacterial agents, applied in the field of microbial agents, can solve the problems of high seawater density, affecting the effect of growth promotion, and few researches and reports on seaweed and seagrass bed ecosystems, so as to achieve a high number of viable bacteria and promote the growth of seagrass , The effect of reducing the number of bacteria

Active Publication Date: 2017-12-01
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies and reports on the application of microbial agents at home and abroad to promote the growth of seagrass and restore the ecosystem of seagrass beds
The existing difficulties are as follows: (1) The particularity of the seaweed growth environment causes the bacterial agent to easily flow with seawater, which affects the actual growth-promoting effect; (2) The high density of seawater is not conducive to the colonization of probiotics in seaweed rhizosphere sediments

Method used

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  • Marine microbial agent and preparation method thereof
  • Marine microbial agent and preparation method thereof
  • Marine microbial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] LB medium: used for strain activation and preservation. Add 10g of peptone, 5g of yeast extract, 25g of NaCl, 1L of deionized water, add 16g of agar to the solid, and then sterilize.

[0023] Selective Seaweed Nitrogen Fixation Medium: Used for primary screening of nitrogen-fixing bacteria. FeSO 4 ·7H 2 O 0.001g, KCl 0.56g, MgCl 2 ·6H 2 O 4.0g, NaCl 25g, MgSO 4 ·7H 2 O 4.8g, K 2 HPO 4 0.01g, Tris 0.48g, peptone 4.0g, yeast extract 2.0g, glycerol 2.0mL, deionized water 1L, pH 8.1, add 16g agar to the solid, and then sterilize.

[0024] Nitrogen-free medium (Ashby): used for re-screening of nitrogen-fixing strains. CaCO 3 5g, Mannitol 5g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O 0.2g, NaCl 5g, CaSO 4 0.1g, aged sea water 0.5L, deionized 0.5L, pH 7.0, then sterilized.

[0025] Improved selective phosphate solubilizing medium (PSM): used for screening of phosphate solubilizing strains. D-glucose 15g, NH 4 NO 3 5g, CaCl 2 2g, KCl 0.5g, MgSO 4 ·7H 2 O 0.5g, ...

Embodiment 2

[0065] Embodiment 2: the preparation of marine microbial bacterial agent

[0066] 1. Bacteria fermentation

[0067] Inoculate Starburr SCSIO 43504 into LB liquid medium for activation for 48 hours, and then inoculate the activated starburr SCSIO 43504 bacterial liquid into 6L fermentation medium 1 [10L fermenter (BioFlo115)] with an inoculum volume fraction of 5% Medium, temperature 33°C, sterile air volume 60m 3 / h, the fermentation time was 60h, and the fermentation broth of Star arrowhead SCSIO 43504 was obtained. Described fermentation medium 1, every liter contains corn flour 10g, soybean flour 5g, NaCl 25g, pH 7.5, and its preparation method is that corn flour 10g, soybean flour 5g, NaCl 25g are added in 1L water, adjust pH value to 7.5, Sterilized derived.

[0068] Inoculate Bacillus SCSIO 43505 into LB liquid medium for activation, then inoculate the activated Bacillus SCSIO43505 bacterium liquid into 6L fermentation medium 2 [10L fermenter (BioFlo 115)] with a volu...

Embodiment 3

[0087] Embodiment 3: the ecological effect of solid microbial agent on the growth of Thaleisis

[0088] 1. Experimental materials and methods

[0089] 1.1. Cultivation of Thalias spp.: In each pot, 20 Thalesias spp. (root length 10 cm, leaves tender green, random length) adapted to 5 days in seawater in situ were cultivated, and the numbers were fixed. At saturation light intensity ((250μmol photons m -2 the s -1 ), cultured at room temperature (30°C). 3 cylinders of the treatment group (length, width and height are 65cm*45cm*35cm) were added with mixed bacterial agent [1g Star Arrowhead SCSIO 43504 bacterial agent (group A in step 3 in embodiment 2, not packed with PVA bag)+1g spore Bacillus SCSIO 43505 inoculum (group F in step 3 in embodiment 2, not packed in PVA bag)], the mixed inoculum is deposited around the root of Thalia spp.; 3 cylinders of the control group are not treated, and the water is changed every 7 days. And add the mixed bacterial agent again, and conti...

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PUM

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Abstract

The invention discloses a marine microbial agent and a preparation method thereof. The marine microbial agent comprises a sagittula stellata SCSIO 43504 agent and bacillus sp. SCSIO 43505 agent. The marine microbial agent disclosed by the invention is high in living bacteria amount, low in foreign bacteria amount and low in fecal coliform amount and can reach the national standard (GB 20287-2006) of the agricultural microbial agent. The marine microbial agent can precipitate around seaweeds in seawater so as to be beneficial to planting strains at the root parts of the seaweeds and playing a role in promoting the growth of the seaweeds.

Description

Technical field: [0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a marine microbial bacterial agent and a preparation method thereof. Background technique: [0002] In tropical and temperate oceans, seagrasses hold a key ecological position due to the large amount of organic carbon fixation and export, nutrient cycling, stable sediments, and rich biodiversity in seagrass bed ecosystems. The seagrass bed ecosystem not only grows a large number of animals and plants, but also parasitizes many microbial populations. Marine nitrogen-fixing prokaryotes can convert atmospheric N elements into ammonium compounds and enter the marine ecosystem; phosphate-dissolving bacteria can dissolve refractory phosphorus compounds and promote the cycle of P elements. However, a survey in 2006 showed that in the past 20 years, due to the threat of natural factors and human factors, about 18% (33000km2) of the recorded seagrass beds in the wor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01C12R1/07
CPCC12N1/20
Inventor 王聪凌娟周卫国林丽云张燕英曾思泉尹浩董俊德
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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