56results about How to "Increase growth density" patented technology

The invention discloses a culture method for high-efficiency human follicle stimulating hormone expression CHO cells and belongs to the technical field of bio-pharmaceuticals. According to the culture method, seed cells are prepared into cell suspension, and subculture is performed after resuspension through a serum-free basal culture medium to obtain seed suspension; the obtained seed suspension is inoculated into a bioreactor to be cultured, the serum-free basal culture medium is fed at regular intervals in the culture process, growth situations of the cells are monitored, glucose solution is supplemented, and the cells are harvested after culture. According to the method, the serum-free basal culture medium is used throughout the process, culture in a fermentation tank is performed after reviving and amplification through bottle shaking, the process flow is simple, the cell culture density is high and can be as high as 4*107 / mL , the cell survival rate reaches more than 98%, maintaining time under high density and a high survival rate is long, the yield of end products can reach 40mg / L, and the method is applicable to industrial production.

The invention provides a method for producing a pseudorabies attenuated vaccine by using a bioreactor and a pseudorabies attenuated vaccine product. After being sterilized, the bioreactor and a micro carrier are inoculated with cells for producing the vaccine, and a cell growth medium is added for culture. A maintenance medium containing attenuated strains of pseudorabies viruses are inoculated into the bioreactor to continue culturing the cells. 2 to 3 days after virus inoculation, cell culture virus liquid is obtained and added with a stabilizer and antibiotics, and the cell culture virus liquid is refrigerated and dried under vacuum to obtain the pseudorabies attenuated vaccine. In the method, the cell density and virus concentration are improved greatly, the titer of the vaccine is improved, the side reactions, labor intensity and product cost are reduced, the monitoring performance of vaccine production is improved and uniform and stable product quality is guaranteed. The pseudorabies attenuated vaccine produced by the method has high safety, immune efficacy and good immune and protective effect against the attack by the virulent pseudorabies viruses.

The invention discloses a method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses through culturing Marc-145 cells by applying a torrent-perfusion bioreactor. The method comprises the following steps of: (1) selecting Marc-145 cells as cells for culturing seedlings; (2) subculturing the Marc-145 cells; (3) reproducing virus seeds for production; (4) perfusing and culturing the Marc-145 cells on a paper carrier in the torrent-perfusion bioreactor; (5) producing the PRRS viruses in the torrent-perfusion bioreactor; and (6) treating the obtained virus antigen solution. The invention can greatly decrease the production cost, improve the input-output ratio by more than 10 times and expand the production scale easily and rapidly, has short production preparation cycle and high degree of automation, occupies less area, causes less environmental pollution which is easy to treat, consumes less labor, is easy to realize balanced and stable virus quality and can obviously improve the yield and the quality of the viruses.

The invention discloses an efficient magnolia grandiflora cultivation method. The method includes the following steps of land selection and preparation, field planting, transplanting, pruning and later period management. By the efficient magnolia grandiflora cultivation method, growth rate of the magnolia grandiflora is increased greatly, survival rate is increased, growth density of magnolia grandiflora plantlets is increased for providing larger growth space for the magnolia grandiflora, applied fertilizer is matched for providing nutritional guarantee to the magnolia grandiflora, pruning and later period management are matched to allow the magnolia grandiflora to obtain enough sunlight and enable full-grown magnolia grandiflora to be more attractive in appearance, and ornamental value and economic value of the magnolia grandiflora are heightened.

The invention relates to a dunaliella salina seawater high-density culture medium which is characterized in that sodium borate is added to the culture medium, and the concentration of the sodium borate is 0.03-0.2 g / L. An experiment proves that the accumulation amount of carotenoid is as high as 304.4 mg / L when the addition amount of the sodium borate is 0.06 g / L, and the accumulation amount of the carotenoid accumulated in dunaliella salina is only 93.3 mg / L when the addition amount of the sodium borate is 0 g / L, so that the addition of the sodium borate is proved to achieve an obvious promoting effect on the accumulation of the carotenoid in the dunaliella salina.

The invention discloses a WAYNE293LVPRO cell adapted to a serum-free culture medium environment and application of the WAYNE293LVPRO cell. The human embryonic kidney cell WAYNE 293 is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 24, 2021, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.22348. The human embryonic kidney cell WAYNE 293 provided by the invention is derived from suspension culture of HEK293 cells in a serum-free culture medium without an anti-caking agent, has the characteristics of short doubling time, high growth density, high virus packaging efficiency and the like, and is an important support for industrial development in the field of cell and gene therapy.

The present invention provides a method for forming a ferroelectric spontaneous polarization reversal capable of forming a ferroelectric spontaneous polarization reversal condition homogeneously within the ferroelectric spontaneous polarization reversal region even across a large region of 50 μm and over in width of a ferroelectric spontaneous polarization reversal. Also, it provides a method for forming a ferroelectric spontaneous polarization reversal where a ferroelectric substrate has convexo-concave structure, such as ridge structure and the like, on its surface and the polarity of the region including one portion of said convex part is reversed with accuracy. A method for forming a ferroelectric spontaneous polarization reversal in a desired region of a ferroelectric substrate 1 has a feature that said desired region 20 of a surface of said ferroelectric substrate is sprayed with micro-hard materials, made an impact by using a striking member that has micro tip diameter, or rubbed with micro-hard materials that are dispersively located on the surface of the substrate to thereby generate nucleuses, and subsequently, a given voltage is applied into said desired region to thereby form a ferroelectric spontaneous polarization reversal in said desired region of said ferroelectric substrate. Also, a method for forming a ferroelectric spontaneous polarization reversal where a convexo-concave structure is formed on a top face of a ferroelectric substrate firstly, and then, a ferroelectric spontaneous polarization region is formed on the substrate including one portion of said convex part, has a feature that a concave portion is formed on a bottom face of the substrate within the region where a ferroelectric spontaneous polarization reversal is to be formed and at least said convex portion is formed, and then, an electric field is applied into said substrate.

The invention relates to an upwelling artificial wetland for removing nitrogen, phosphorus and antibiotics in aquaculture wastewater, designs sewage treatment equipment which is an ecological treatment device of an artificial wetland and suitable for removing combined pollutions caused by the nitrogen, the phosphorus and the antibiotics in the aquaculture wastewater, and belongs to a vertical flow artificial wetland device. According to the upwelling artificial wetland, oyster shells, waste bricks and red earth, which have strong nitrogen and phosphorus adsorption capacity and high microorganism containing quantity, and are taken as main wetland fillers; wetland plant reeds which have good pollution resistance are planted. The upwelling is taken as a hydraulic transmission mode, so that the problems of hydraulic dead zone, insufficient nitrogen removal ability, poor antibiotic interception ability and poor rainstorm impact resistance existing in the traditional operation mode are effectively solved. Meanwhile, the upwelling artificial wetland has the advantages of being low in cost, small in land occupation, simple in structure, convenient in operation management and the like.

The invention provides a parallel bioreactor system. The parallel bioreactor system comprises: an oscillator for generating oscillatory motion; a plurality of culture containers mounted on the oscillator, wherein each culture container has an inner chamber, every inner chamber comprises an upper cylindrical part and a lower inverted frustoconical bottom part, the cross section of the cylindrical part coincides with the top cross section of the inverted frustoconical bottom part, and the bottom of the cylindrical part is connected with the top of the inverted frustoconical bottom part; disposable culture bags arranged in the inner chambers of the culture container and used for accommodating a culture solution, wherein each disposable culture bag has a multifunctional cover plate, the multifunctional cover plates are connected to the tops of the culture bags to seal the culture bags, and the multifunctional cover plates are provided with a plurality of access holes communicating with theinternals of the disposable culture bags; and a control system, wherein the control system controls the oscillatory motion of the oscillator and the parameters of the culture solution in the disposable culture bags.

The invention discloses a bifidobacterium longum as well as application and a product thereof. The bifidobacterium longum is classified and named as Bifidobacterium longum 5b5d1 and is preserved in the China Center for Type Culture Collection (CCTCC) on April 16, 2021, and the preservation number of the bifidobacterium longum is CCTCC M 2021390. The bifidobacterium longum 5b5d1 with high growth rate and high growth density is obtained through open high-throughput strain screening, the fermentation time can be shortened, the production cycle can be shortened, meanwhile, the yield of a fermentation product can be increased, and the economic benefit is huge. The fermentation product is proved to have an epithelial tissue repairing effect, and can be applied to preparation of medicines, foods and cosmetics including skin repairing preparations.

The invention discloses a soilless culture nutrient solution for sagittaria sagittifolia. The soilless culture nutrient solution comprises the following components in parts by weight: 50-60 parts of a nitrogen-potassium-potassium fertilizer, 20-40 parts of calcium nitrate, 15-25 parts of magnesium sulfate, 15-20 parts of microorganisms, 30-35 parts of trace elements, 45-65 parts of cattle biogas slurry, 100 parts of water, 1-5 parts of a bactericide and 1-5 parts of a pH adjusting agent. The soilless culture nutrient solution for sagittaria sagittifolia can provide nutrition needed for sagittaria sagittifolia to grow for a long time, can reduce the planting cost, can increase the yield and improve the quality and the security of sagittaria sagittifolia, and is applicable to large-scale production.

The invention provides a haematococcus pluvialis culture medium. The culture medium per liter contains 0.30-0.40g of NaNO3, 0.10-0.20g of KNO3, 0.07-0.18g of MgSO4.7H2O, 0.03-0.06g of Na2SO3, 0.10-0.30g of C6H5Na3O7, 0.02g of inorganic phosphorus, 0.005-0.02g of organic phosphorus, 0.03-0.09g of CaC12, 0.0002-0.0003g of EDTA-NaFe, 0.001-0.003g of FeSO4, 0.0015-0.0045g of ZnSO4, 0.00006-0.00018g of MnCl2, 0.00003-0.0001g of CoCl2, 0.0001-0.0003g of H3BO3, 0.00006-0.00018g of CuSO4, 0.00002-0.00006g of (NH4) Mo7O24, 0.002g of 3-IBA, 0.0003g of 6-BA and 0.000025-0.00005g of Vb12. The culture medium is used for photoautotrophically culturing haematococcus pluvialis, can significantly promote the growth of haematococcus pluvialis, enables the haematococcus pluvialis to rapidly enter the exponential growth phase so as to achieve higher growth density and keep the dominant position of the population of haematococcus pluvialis in a short time, and therefore is conducive to furthest reducing pollution probabilities of foreign bacteria, seaweeds and protozoa, shortening the growth cycle and increasing the successful probability of culture in the field enlarging culture process.

The invention provides a preparation method of a CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and a product of the CSF and PR bivalent live vaccine. The method comprises the following steps of culturing a CSF virus lentogen strain and a PR virus lentogen strain by a cell roller bottle or a bioreactor; harvesting obtained cell culture virus solutions; mixing the two kinds of obtained cell culture virus solutions according to a certain volume percent; adding a stabilizing agent and antibiotics into the mixed solution; and performing freeze-drying to obtain the CSF and PR bivalent live vaccine. The CSF and PR bivalent live vaccine is used for immunization; the workload of the immunization can be reduced; the immunization times can be reduced; the immunologic paralysis due to frequent immunization is avoided; the stress on swinery is correspondingly reduced; and the goal of preventing two kinds of diseases by one vaccine is achieved.

The invention relates to a method for preparing a cell culture vector, which comprises the following steps: firstly, preparing nanoscale silk fibroin powder; secondly, dispersing or dissolving the nanoscale silk fibroin powder in an aqueous solution to blend with an aqueous solution of polyvinyl alcohol, and forming the blending solution into a fiber or a membrane; and finally, making the fiber or the membrane into a required three-dimensional shape to obtain the cell culture vector. The preparation method has the advantage of easy industrial application; and cells cultured by the obtained cell culture vector have the characteristics of high density, high yield and low cost. The cell culture vector prepared by the method is used for culturing target cells, and the cultured target cells can be used for gene expression and preparation of genomic medicaments. In addition, the cell culture vector also can be used for a tissue engineering bracket.

The invention belongs to the field of soil restoration treatment, in particular to an ecological restoration system and restoration agent and a method for restoring soil, treating saline-alkali soil and removing heavy metal pollution. Especially, eucommia ulmoides is planted in a polluted environment, soil is irrigated with sewage, a composition of indole butyric acid and dimethyl carbonate is applied, it is promoted that pollutants are absorbed by the eucommia ulmoides after being decomposed, xylem is synthesized, the pollutant and organic matter decaying can be fundamentally eliminated, foul smells are fundamentally eliminated, and pollution is reduced.

A method for forming a ferroelectric spontaneous polarization reversal in a desired region of a ferroelectric substrate includes the steps of forming, for the desired region of the surface of the ferroelectric substrate, an electrode pattern or a mask pattern composed of aggregates of micropatterns, and then applying a given voltage into the desired region. The configuration of the micropatterns can be a stripe-shaped pattern, an ellipse-shaped pattern, a hexagon-shaped pattern, a network pattern, or a double cross shaped pattern. The method can further include the steps of generating many nucleuses by using the electrode pattern or the mask pattern composed of the aggregates of micropatterns, forming another electrode pattern or another mask pattern corresponding to the desired region, and then applying a given voltage into the desired region to generate a ferroelectric spontaneous polarization reversal around the nucleuses.

The invention discloses a high-yield cultivation method for potato high-ridge close planting. The method includes the following steps that firstly, a sandy planting field is selected, deep scarification and ridging are conducted, ditches are communicated with each other, and the furrow ditch is deeper than other ditches; secondly, 500-750 kg of composted chicken manure or 300-500 kg of organic composite fertilizer with the composted chicken manure as the raw material, 75 kg of long-acting controlled-released composite fertilizer, 40-45 kg of quick-acting composite fertilizer, 2.5 kg of magnesium sulfate and 1 kg of zinc sulfate and agricultural borax are applied to each mu of planting field, and soil covering is conducted after the fertilizers are applied to the ditches in furrows; thirdly, seed potatoes are purchased and diced, wherein each potato dice is 30-50 g heavy, and 1-2 holes are formed in each dice; fourthly, the seed potatoes are sown from the late October to late November at the temperature of 17-21 DEG C, and 4000-5000 plants are sown in each mu; fifthly, soil is watered and wetted 4-7 days after sowing, the soil is irrigated with a proper amount of water according to actual conditions in the seedling period, accumulated water is drained 20 days before harvesting, and the situations that rotten potatoes are caused due to accumulated water and ditches need to be drained when the amount rainwater is excessively large are prevented; sixthly, when stems and leaves of potatoes become yellow and after potato skin is lignified, the potatoes can be harvested. The method increases the potato yield.

The invention discloses a method for producing PRRS (Porcine Reproductive and Respiratory Syndrome) viruses through culturing Marc-145 cells by applying a torrent-perfusion bioreactor. The method comprises the following steps of: (1) selecting Marc-145 cells as cells for culturing seedlings; (2) subculturing the Marc-145 cells; (3) reproducing virus seeds for production; (4) perfusing and culturing the Marc-145 cells on a paper carrier in the torrent-perfusion bioreactor; (5) producing the PRRS viruses in the torrent-perfusion bioreactor; and (6) treating the obtained virus antigen solution. The invention can greatly decrease the production cost, improve the input-output ratio by more than 10 times and expand the production scale easily and rapidly, has short production preparation cycle and high degree of automation, occupies less area, causes less environmental pollution which is easy to treat, consumes less labor, is easy to realize balanced and stable virus quality and can obviously improve the yield and the quality of the viruses.

The invention belongs to the field of harmless landfill treatment of solid waste, and particularly relates to an ecological system, method and agent for harmless landfill treatment of household refuse, industrial solid waste residues and livestock contaminants. In refuse landfill, by using the harmless landfill treatment agent which is prepared from indolebutyric acid with the concentration of 30-3000 mg / L and nitrohumic acid with the concentration of 20-3400 mg / L, decomposition of contaminants can be accelerated, absorption and utilization of contaminants by plants can be promoted, foul smells can be eliminated fundamentally, and waste gas can be treated / eliminated fundamentally. The safety problems of leachate leakage, degradation gas leakage, foul smell dispersion and pathogenic bacterium leakage which are likely to occur in the prior art are avoided.

The invention discloses an application of arabidopsis thaliana zinc finger protein gene ZFP6 to promoting the growth of tobacco glandular hair. The nucleotide sequence of the arabidopsis thaliana zinc finger protein gene ZFP6 is shown as SEQ ID NO. 1 (in the Specification). The application comprises the steps as follows: the arabidopsis thaliana zinc finger protein gene ZFP6 is connected to a plant expression carrier; a recombined expression carrier is obtained via construction; the recombined expression carrier is converted into tobacco. According to the application, the arabidopsis thaliana zinc finger protein gene ZFP6 is cloned, and is subjected to heredity conversion in tobacco; overly expressing outer source ZFP6 gene can remarkably improve the number of tobacco leaf glandular hair, and the number of protective hair not generating secondary metabolism product is not affected. The application disclosed by the invention conduces to the research of the regulation and control mechanism of glandular hair formation and development and the heredity law of glandular hair density, improvement on the quality of aroma of tobacco, and research and control of the formation mechanism of tobacco glandular hair and aroma formation.

The invention discloses a preparation method of a hydrophobia inactivated vaccine. The preparation method comprises the following steps of carrying out cell recovery, generation and culture on a hamster kidney cell system, carrying out virus seed reproduction and venom reproduction on the treated hamster kidney cells, preparing a vaccine adjuvant, adding B-propiolactone according to a proportion of 0.025% of the total amount of the venom, inactivating at 4 DEG C for 24h, hydrolyzing at 36 DEG C for 2h and quantificationally sub-packaging the hydrolyzed cell venom and vaccine adjuvant according to a proportion of 9: 1 to obtain the hydrophobia inactivated vaccine. The preparation method of the hydrophobia inactivated vaccine has the advantages that the operation is simple, the process is stable, the virus content of the vaccine is high and the quality is easy to control, and is capable of remarkably improving the yield and quality of the vaccine; the vaccine prepared by the method is good in safety, high in immunity and low in cost.

The invention discloses a method for growing a large-scale IV-VI group compound single crystal thin film material by a PVD technology. The method specifically comprises three main stages: 1, preparing a material, and optimally selecting the weight of a polycrystalline powder source; 2, placing the powder source and a substrate in a quartz tube, and setting the positions of the powder source and the substrate in the quartz tube; and 3, growing a single crystal film by utilizing the physical vapor deposition technology. A large-scale layered film material with a regular strip shape is prepared by optimizing the heating rate, the heat preservation temperature and time, the substrate temperature and the airflow velocity of a powder source, so that an important technical foundation is laid for experimental preparation and application of a large-scale IV-VI family single crystal film.

The invention provides a formula of grass seeds with high treading resistance. The grass seeds are prepared from, by mass, 40% of festuca arundinacea seeds, 20% of bluegrass seeds and 40% of ryegrassseeds. The festuca arundinacea seeds are proportionally prepared from, by mass, 35% of Yaosai grass seeds, 30% of Chengbao grass seeds and 35% of Xuehu grass seeds. The bluegrass seeds are proportionally prepared from, by mass, 35% of bluegrass Wushi grass seeds, 35% of bluegrass Field and 30% of bluegrass Mecui. The ryegrass seeds are proportionally prepared from, by mass, 55% of Zhuozhuang grassseeds and 45% of Shenshi grass seeds. The formula can effectively improve the treading resistance of a lawn.

The invention belongs to the field of sewage treatment, relates to soil remediation and slag treatment integrated techniques, and particularly relates to a multi-field sewage treatment and soil and slag remediation integrated ecological system, a method, a remediation agent and a treatment agent to realize integration of soil and slag remediation and treatment of multi-field sewage such as domestic sewage, industrial sewage, livestock breeding sewage, papermaking sewage, high-salt sewage and high-organic-content sewage. A scheme includes that in plant planting, indolebutyric acid and cocoamidopropyl betaine are adopted for treating pollutants before plant planting, deodorizing and waste gas treatment / elimination can be realized, the pollutants are utilized in the industrial field by a shortest ecological cycle chain, waste recycling is realized, biological cycle efficiency is improved, and ecological system recovery is realized.

The invention belongs to the field of environment pollution governing, and particularly relates to a technology for eliminating odor and thoroughly eliminating waste gas during the sewage and garbage treatment and soil restoration, and particularly relates to an agent and a regulating agent for eliminating odor and treating waste gas, an ecological treatment system and a method. Gutta percha trees are planted in a pollution environment; a composition of indolebutyric acid and nitrohumic acid is applied onto the gutta percha trees so that pollutants are promoted to be decomposed and to be then absorbed by the gutta percha trees to synthesize xylogens and the like; the pollutants and the organic matters can be fundamentally eliminated; the odor is fundamentally eliminated; the pollution is reduced.

The invention belongs to a combination technology for sewage treatment and soil remediation, and particularly relates to an integrated ecological remediation system combining soil remediation and sewage treatment, a remediation conditioning agent and a treating agent. By means of the integrated ecological remediation system combining soil remediation and sewage treatment, the remediation conditioning agent and the treating agent, lands of a refuse landfill, slag mountains, lands infected by heavy metal and lands infected by organics are remediated while urban sewage, high salinity wastewater, industrial wastewater, food industry wastewater, paper industry wastewater and livestock breeding slaughtering wastewater are treated. The system is composed of eucommia ulnoides planting forests, pigs and / or fishes. The system can purify sewage and remediate soil at the same time, pollutants are utilized in the industrial field with the shortest biological cycle chain, waste is turned into wealth, the biological cycle efficiency is improved, and the ecological system is restored.

The invention discloses a method for culturing diatom in summer. The method comprises the following steps: obtaining eutrophic water formed by rotting and fermenting natural organic matter, and regulating the transparency of the eutrophic water; transferring the eutrophic water into a culture container, and properly shading the culture container to form a relatively weak light condition; and culturing the eutrophic water in the culture container by utilizing the high-temperature condition in summer.