Method for preparing cell culture vector

Abstract

The invention relates to a method for preparing a cell culture vector, which comprises the following steps: firstly, preparing nanoscale silk fibroin powder; secondly, dispersing or dissolving the nanoscale silk fibroin powder in an aqueous solution to blend with an aqueous solution of polyvinyl alcohol, and forming the blending solution into a fiber or a membrane; and finally, making the fiber or the membrane into a required three-dimensional shape to obtain the cell culture vector. The preparation method has the advantage of easy industrial application; and cells cultured by the obtained cell culture vector have the characteristics of high density, high yield and low cost. The cell culture vector prepared by the method is used for culturing target cells, and the cultured target cells can be used for gene expression and preparation of genomic medicaments. In addition, the cell culture vector also can be used for a tissue engineering bracket.

Description

technical field [0001] The invention relates to a method for preparing a cell culture carrier, specifically referring to a method for preparing a cell culture carrier by using nano-scale silk fibroin particles (or fragments) and polyvinyl alcohol. The cell culture carrier prepared by the method is used for cultivating target cells. The obtained target cells can be used for gene expression and gene medicine preparation, and the cell culture carrier can also be used for tissue engineering scaffolds, belonging to the fields of biomaterials, biochemical engineering and cell engineering. Background technique [0002] Silk fibroin can support cell adhesion, proliferation and differentiation in vitro in various forms (such as thin film, membrane, network structure, sponge, etc.), and promote tissue repair in vivo, especially the three-dimensional silk fibroin scaffold expands the The application of element in cell tissue engineering has become one of the most promising biomaterials...

AI Technical Summary

Problems solved by technology

The types of existing in vitro cultured cells include adherent cells and suspension cells. The culture methods mainly include primary culture, subculture, suspension culture and others such as microcarriers, microcapsules, hollow fiber methods, etc., and the cells used Most of the matrix materials are synthetic polymer materials, which have poor biocompatibility, and synthetic polymer materials may also have residual monomers with certain toxicity

At the same time, it has problems such as surface hydrophobicity or surface roughness, and the cultured cell density is low and the yield is small. If the surface is modified again to improve the surface hydrophilicity, the cost is high and other disadvantages.