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Preparation method of CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and product of CSF and PR bivalent live vaccine

A technology of pseudorabies and pseudorabies virus, applied in the field of veterinary biological products, can solve the problems of difficulty in rationally arranging various immunization procedures, affecting the prevention effect, increasing costs, etc., to achieve uniform and stable product quality, improve vaccine production and quality, cost-increasing effect

Inactive Publication Date: 2016-05-18
广东永顺生物制药股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many varieties of vaccines for pigs. In actual operation, multiple injections, multiple doses, and multiple immunization procedures are difficult to rationally arrange. It is cumbersome, time-consuming, laborious, and increases costs. It is also easy to cause missed seeds, which directly affects the prevention effect.

Method used

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  • Preparation method of CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and product of CSF and PR bivalent live vaccine
  • Preparation method of CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and product of CSF and PR bivalent live vaccine
  • Preparation method of CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and product of CSF and PR bivalent live vaccine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation of embodiment 1 swine fever, pseudorabies dual live vaccine

[0035] Such as figure 1 Shown, the preparation method of swine fever, pseudorabies dual live vaccine, technical scheme is:

[0036] (1) Culture method: use bioreactor or cell roller bottle to cultivate cells and propagate classical swine fever virus and pseudorabies virus.

[0037] (2) Select susceptible cells as cells for seedling production: one of bovine testis cells, chicken embryo fibroblasts, porcine testis (ST) passage cells, pig kidney (PK15) passage cells, and pig kidney (IBRS-2) passage cells .

[0038] (3) Passage and culture of cells for seedling production: the above-mentioned susceptible cells are digested and passed by EDTA-trypsin cell dispersion liquid, and continued to be cultured with cell growth liquid. When a good monolayer is formed, they are used for continued passage or virus inoculation;

[0039] (4) Propagation of poisonous species for production:

[0040] A. Pro...

Embodiment 2

[0087] The immune test of embodiment 2 swine fever, pseudorabies dual live vaccine to pig

[0088] 1 Materials and methods

[0089] 1.1 Materials

[0090] 1.1.1 The live vaccine of swine fever and pseudorabies is produced by Guangdong Yongshun Biopharmaceutical Co., Ltd. and passed the inspection.

[0091] 1.1.2 The pigs used in the test are from the binary hybrid pigs self-propagating and self-raised in the experimental animal farm of Guangdong Yongshun Biopharmaceutical Co., Ltd., and have no history of CSFV, PRRSV, PRV, PPV, PCV-Ⅱ, and have never been vaccinated against CSF vaccine or PR vaccine. and 20 healthy pigs with PRRS vaccine. After detection by ELISA and neutralization test methods, no neutralizing antibodies to swine fever and pseudorabies were selected.

[0092]1.1.3 The swine fever antibody detection kit was purchased from IDEXX company.

[0093] 1.1.4 A pseudorabies antibody detection kit was purchased from IDEXX Company.

[0094] 1.2 Method

[0095] 1.2....

Embodiment 3

[0109] Comparative experiment of embodiment 3 swine fever, pseudorabies dual live vaccine and swine fever live vaccine, pseudorabies live vaccine

[0110] 1 material

[0111] 1.1 3 batches of live vaccines for swine fever and pseudorabies used in the test, batch numbers: CSF-PR200801, CSF-PR200802, CSF-PR200803, passed the inspection according to the current "Chinese Veterinary Pharmacopoeia" inspection method;

[0112] Three batches of live swine fever vaccines, batch numbers: CSF200801, CSF200802, and CSF200803, passed the inspection according to the current "Chinese Veterinary Pharmacopoeia" "Swine Fever Live Vaccine (Cell Source)" inspection method;

[0113] Three batches of live pseudorabies vaccine, batch numbers: PR200801, PR200802, PR200803, passed the test according to the current "Chinese Veterinary Pharmacopoeia" "Pseudorabies Live Vaccine".

[0114] 1.2 Experimental animals

[0115] 1.2.1 Rabbits come from New Zealand big-eared whites that are self-propagating an...

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Abstract

The invention provides a preparation method of a CSF (Classical Swine Fever) and PR (Pseudorabies) bivalent live vaccine and a product of the CSF and PR bivalent live vaccine. The method comprises the following steps of culturing a CSF virus lentogen strain and a PR virus lentogen strain by a cell roller bottle or a bioreactor; harvesting obtained cell culture virus solutions; mixing the two kinds of obtained cell culture virus solutions according to a certain volume percent; adding a stabilizing agent and antibiotics into the mixed solution; and performing freeze-drying to obtain the CSF and PR bivalent live vaccine. The CSF and PR bivalent live vaccine is used for immunization; the workload of the immunization can be reduced; the immunization times can be reduced; the immunologic paralysis due to frequent immunization is avoided; the stress on swinery is correspondingly reduced; and the goal of preventing two kinds of diseases by one vaccine is achieved.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a preparation method of a dual live vaccine against swine fever and pseudorabies and its products. Background technique [0002] Classical swine fever (CSF) and pseudorabies (PR) are two important infectious diseases that harm the intensive pig industry. They exist widely in pig herds in my country and cause huge economic losses to the pig industry. Immunization is an important measure for the prevention and control of swine fever and pseudorabies. At present, the application of attenuated classical swine fever rabies vaccine and live pseudorabies vaccine has played a significant role in the prevention and control of these two diseases. However, there are many varieties of vaccines for pigs. In actual operation, it is difficult to rationally arrange multiple injections, multiple doses, and multiple immunization procedures. It is cumbersome,...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/245A61K39/187A61P31/14A61P31/20
Inventor 吴文福宁宜宝林旭埜张毓金岑小清游启有任向阳张国丽丘成文杨傲冰赖月辉
Owner 广东永顺生物制药股份有限公司
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