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Bifidobacterium longum as well as application and product thereof

A Bifidobacterium longum, daily-use technology, applied in the field of microorganisms, can solve the problems of low production efficiency, low strain growth rate, long production cycle, etc.

Active Publication Date: 2021-07-06
君维安(武汉)生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, Bifidobacterium longum belongs to strict anaerobic bacteria, the growth rate of bacterial strains is low during the fermentation process, the production cycle is long, and the production efficiency is low

Method used

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  • Bifidobacterium longum as well as application and product thereof
  • Bifidobacterium longum as well as application and product thereof
  • Bifidobacterium longum as well as application and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The laboratory collected milk samples from healthy lactating women. After gradient dilution with sterile anaerobic water, the appropriate gradient dilution was selected and spread on the MRS solid medium, and anaerobically incubated at 37°C for 48 hours. Pick typical colonies and streak them on MRS solid medium for 2-3 times, and cultivate to obtain pure colonies. It was identified as a strain of Bifidobacterium longum by 16S rDNA molecular identification, and it was named Bifidobacterium longum 5b5d1 (Bifidobacterium longum 5b5d1).

Embodiment 2

[0037] Embodiment 2 bifidobacterium longum growth curve comparison

[0038] Experimental Materials:

[0039] 1. Strains: Bifidobacterium longum 5b5d1 and Bifidobacterium longum (R175) isolated in Example 1 were isolated from DuPont raw materials containing Bifidobacterium longum R175, identified as Bifidobacterium longum by 16S rDNA molecules, named as Bifidobacterium longum Mycobacterium R175.

[0040] 2. Strain medium: MRS medium.

[0041] Growth curve drawing steps:

[0042] S1, strain activation

[0043] Take out the glycerin cryopreservation tube of Bifidobacterium longum stored in the -80°C refrigerator, put it at room temperature to thaw, use the inoculation loop to pick the bacteria in a sterile environment, inoculate it on the MRS solid plate, and incubate at 37°C for 48 hours. After observing the colony morphology on the plate and confirming that it is Bifidobacterium longum without pollution, pick a single bacterium on the plate and inoculate it into MRS liquid ...

Embodiment 3

[0056] Example 3 Fermentation broth cell function evaluation

[0057] 1, the fermented liquid cytotoxicity evaluation prepared in embodiment 2:

[0058] Take 3×10 4 Density of cells / well Seed HSF cells into 96-well plates, culture at 37°C, 5% CO2 for 24 hours, suck out the supernatant and group them according to the experiment

[0059] Experimental blank group: 100 μL of DMEM (serum-free) medium containing 5% of the Bifidobacterium longum fermentation broth A, B, C, and D prepared in Example 2 was added to the wells without cells

[0060] Experimental group: Add 100 μL of DMEM (serum-free) medium containing 5% Bifidobacterium longum fermentation broth A, B, C, and D prepared in Example 2 to the wells with cells

[0061] Control blank group: add 100 μL DMEM (serum-free) medium to the cell-free well

[0062] Control group: Add 100 μL DMEM (serum-free) medium to the wells with cells

[0063] Cultivate at 37°C and 5% CO2 for 24 hours, remove the supernatant, add 100 μL of medi...

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Abstract

The invention discloses a bifidobacterium longum as well as application and a product thereof. The bifidobacterium longum is classified and named as Bifidobacterium longum 5b5d1 and is preserved in the China Center for Type Culture Collection (CCTCC) on April 16, 2021, and the preservation number of the bifidobacterium longum is CCTCC M 2021390. The bifidobacterium longum 5b5d1 with high growth rate and high growth density is obtained through open high-throughput strain screening, the fermentation time can be shortened, the production cycle can be shortened, meanwhile, the yield of a fermentation product can be increased, and the economic benefit is huge. The fermentation product is proved to have an epithelial tissue repairing effect, and can be applied to preparation of medicines, foods and cosmetics including skin repairing preparations.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a bifidobacterium longum and its application. Background technique [0002] Bifidobacterium longum, a substance containing a variety of bifidobacterium longum metabolite components obtained through cultivation and filtration. The substance contains amino acids, lipids, polysaccharides, adenosine, vitamins, trace minerals and vitamin B groups, etc. The active ingredients are mild and safe to the human body, and are easily absorbed and utilized by epithelial tissues, such as skin, intestinal mucosa, and conjunctiva. This active substance obtained by using biotechnology can help epithelial tissue fight against harmful pathogens and maintain micro-ecological balance, such as skin surface micro-ecological balance; it can moisturize and nourish damaged epithelial tissue, regulate its immunity, and help resist the external environment and Damage to the skin caused by oxida...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/745A61P17/00A61K8/99A61Q19/00A23L33/135C12R1/01
CPCC12N1/20A61K35/745A61P17/00A61K8/99A61Q19/00A23L33/135A61K2800/85A23V2002/00A23V2400/533A23V2200/318
Inventor 刘智舒婷聂庆庆陈嘉敏王敏
Owner 君维安(武汉)生命科技有限公司
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