Haematococcus pluvialis culture medium
A technology of Haematococcus pluvialis and culture medium, which is applied in the directions of microorganisms, unicellular algae, microorganism-based methods, etc., can solve the problem that there is no research on the effect of organic phosphorus sources on the growth of Haematococcus, and there is no regulation of Haematococcus cells. growth and other issues, to shorten the growth cycle, improve the probability of success, and increase yield.
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Embodiment 1
[0042] (1) Each liter of medium contains
[0043] NaNO 3 0.32 g, KNO 3 0.17 g, MgSO 4 ·7H 2 O 0.07g, Na 2 SO 3 0.032g, C 6 h 5 Na3 o 7 0.11 g, KH 2 PO 4 0.02 g, G-P 0.015 g, CaCl 2 0.03 g, EDTANaFe 0.0002 g, FeSO 4 0.001 g, ZnSO 4 0.0015 g, MnCl 2 0.00006 g, CoCl 2 0.00003 g, H 3 BO 3 0.0001 g, CuSO 4 0.00006 g, (NH 4 )Mo 7 o 24 0.00002 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.
[0044] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three replica...
Embodiment 2
[0052] (1) Each liter of medium contains
[0053] NaNO 3 0.35 g, KNO 3 0.15 g, MgSO 4 ·7H 2 O 0.14 g, Na 2 SO 3 0.05 g, C 6 h 5 Na 3 o 7 0.20 g, KH 2 PO 4 0.02 g, G-P 0.010 g, CaCl 2 0.06 g, EDTANaFe 0.00025 g, FeSO 4 0.002 g, ZnSO 4 0.0030 g, MnCl 2 0.00012 g, CoCl 2 0.00007 g, H 3 BO 3 0.0002 g, CuSO 4 0.00012 g, (NH 4 )Mo 7 o 24 0.00004 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.
[0054] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three rep...
Embodiment 3
[0061] (1) Each liter of medium contains
[0062] NaNO 3 0.34 g, KNO 3 0.16 g, MgSO 4 ·7H 2 O 0.13 g, Na 2 SO 3 0.045 g, C 6 h 5 Na 3 o 7 0.22 g, KH 2 PO 4 0.02 g, G-P 0.02 g, CaCl 2 0.07 g, EDTANaFe 0.00024 g, FeSO 4 0.0018 g, ZnSO 4 0.0024 g, MnCl 2 0.00015 g, CoCl 2 0.00005 g, H 3 BO 3 0.00019 g, CuSO 4 0.00014 g, (NH 4 )Mo 7 o 24 0.00003 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.
[0063] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three r...
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