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Dunaliella salina seawater high-density culture medium and culture method

A technology of Dunaliella salina and a culturing method is applied in the field of Dunaliella salina seawater high-density culture medium and culture, and can solve problems such as adverse growth reaction, high cost and slow growth rate of Dunaliella salina.

Inactive Publication Date: 2015-03-04
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method does not fully consider the addition of NH 4 + The effect on the growth of salina, many studies have shown that NH 4 + It has a certain killing effect on salina, especially under the condition of long-term effect, the growth of salina will have obvious adverse reactions
[0008] Most of the culture methods reported in the literature at present use f / 2 medium or Modified Johnson's medium, or make slight optimizations on this basis, which have not solved the problem of slow growth of Salina salina, susceptibility to contamination by bacteria and pests. , high cost and other disadvantages

Method used

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  • Dunaliella salina seawater high-density culture medium and culture method
  • Dunaliella salina seawater high-density culture medium and culture method
  • Dunaliella salina seawater high-density culture medium and culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The cultivation of salina and the effect of adding different concentrations of borax on the growth rate of salina

[0054] Configure sea salt medium with different concentrations of borax, the concentrations are set to 0, 0.03, 0.06, 0.09, 0.12, 0.15, 0.18, 0.20g / L, respectively, Modified Johoson’s medium is used as a control, and Salina is treated according to OD 430 The density of 0.1 was inoculated in the sea salt medium, and the salt algae were cultivated at 27°C, the light intensity was 3700Lux, the light source was a fluorescent lamp, and the light time was 16h / day, so as to detect the effect of different concentrations of borax on the growth rate of the salt algae. influences.

[0055] Check the medium OD every two days 430 change, and according to the corresponding curve of absorbance and the number of salina, the growth curve of salina growth is obtained, such as figure 1 As shown, by calculation, the growth rate of Salina salina is the largest when the conce...

Embodiment 2

[0057] Effects of Adding Different Concentrations of Borax on the Biomass of Salina

[0058] Configure sea salt medium with different concentrations of borax, the concentrations are set to 0, 0.03, 0.06, 0.09, 0.12, 0.15, 0.18, 0.20g / L, respectively, Modified Johoson’s medium is used as a control, and Salina is treated according to OD 430 Inoculate the salt algae at a density of 0.1 in sea-salt culture medium at 27°C, with a light intensity of 3600-4000 Lux, a fluorescent light source, and a lighting time of 16 hours per day to cultivate Salina salina to detect the effects of different concentrations of borax on the growth of Salina salina. Quantitative impact.

[0059] When the growth of salina enters the stable period, take 200ml of algae liquid containing different concentrations of borax, centrifuge at 5000rpm / min for 10 minutes, discard the supernatant, and dry the algae in an oven at 90-95°C for 6- 8 hours to constant weight, obtain the biomass of Salina, such as figu...

Embodiment 3

[0061] Nutrient consumption and addition during the growth of salina

[0062] Prepare a sea salt medium with a borax concentration of 0.06g / L, and dilute algae according to the OD 430 Inoculate in sea salt medium at a density of 0.1, cultivate Salina salina at 27°C, light intensity 3700Lux, fluorescent light source, light time 16h / day, and measure KNO in the medium every other day 3 、KH 2 PO 4 content, and record the number of Salina, and finally get Salina consumption KNO 3 、KH 2 PO 4 rate, as shown in Figure 3. According to KNO 3 、KH 2 PO 4 Consumption rate, determine its addition amount is 0.02772mg / 10 6 each, 0.09498mg / 10 6 , NaHCO 3 The supplementary amount is 0.84g / L / day.

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Abstract

The invention relates to a dunaliella salina seawater high-density culture medium which is characterized in that sodium borate is added to the culture medium, and the concentration of the sodium borate is 0.03-0.2 g / L. An experiment proves that the accumulation amount of carotenoid is as high as 304.4 mg / L when the addition amount of the sodium borate is 0.06 g / L, and the accumulation amount of the carotenoid accumulated in dunaliella salina is only 93.3 mg / L when the addition amount of the sodium borate is 0 g / L, so that the addition of the sodium borate is proved to achieve an obvious promoting effect on the accumulation of the carotenoid in the dunaliella salina.

Description

technical field [0001] The invention relates to a method for cultivating algal plants, in particular to a seawater high-density culture medium for Dunaliella salina and a method for cultivating it. Background technique [0002] Dunaliella salina, also known as salina, is a cell-wallless, flagellated, unicellular algae and is one of the most salt-tolerant eukaryotes. Salina has a long history of cultivation in China, and it has the characteristics of fast growth and large accumulation of beneficial metabolites. Salina can accumulate a large amount of β-carotene in a stress environment (high temperature, high salt and / or high light), up to 14% of the dry weight of algal cells, and is one of the organisms with the highest β-carotene content in nature. Studies have shown that carotenoids are not only the precursors of vitamin A and natural pigments in the food processing industry, but also prevent cancer, tumors and cardiovascular diseases, as well as age-related degenerative d...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 吕和鑫崔相敢贾士儒夏锋
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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