Haematococcus pluvialis culture medium
A technology of Haematococcus pluvialis and culture medium, applied in the direction of microorganisms, single-cell algae, and methods based on microorganisms, can solve the research on the effect of organic phosphorus sources on the growth of Haematococcus, and does not regulate the growth of Haematococcus cells. Growth and other problems, to shorten the growth cycle, increase the probability of success, increase the effect of yield
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Embodiment 1
[0040] (1) Each liter of medium contains
[0041] NaNO 3 0.32 g, KNO 3 0.17 g, MgSO 4 ·7H 2 O 0.07g, Na 2 SO 3 0.032g, C 6 h 5 Na 3 o 7 0.11 g, KH 2 PO 40.02 g, G-P 0.015 g, CaCl 2 0.03 g, EDTANaFe 0.0002 g, FeSO 4 0.001 g, ZnSO 4 0.0015 g, MnCl 2 0.00006 g, CoCl 2 0.00003 g, H 3 BO 3 0.0001 g, CuSO 4 0.00006 g, (NH 4 )Mo 7 o 24 0.00002 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.
[0042] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three replica...
Embodiment 2
[0047] (1) Each liter of medium contains
[0048] NaNO 3 0.35 g, KNO 3 0.15 g, MgSO 4 ·7H 2 O 0.14 g, Na 2 SO 3 0.05 g, C 6 h 5 Na 3 o 7 0.20 g, KH 2 PO 4 0.02 g, G-P 0.010 g, CaCl 2 0.06 g, EDTANaFe 0.00025 g, FeSO 4 0.002 g, ZnSO 4 0.0030 g, MnCl 2 0.00012 g, CoCl 2 0.00007 g, H 3 BO 3 0.0002 g, CuSO 4 0.00012 g, (NH 4 )Mo 7 o 24 0.00004 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.
[0049] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three rep...
Embodiment 3
[0054] (1) Each liter of medium contains
[0055] NaNO 3 0.34 g, KNO 3 0.16 g, MgSO 4 ·7H 2 O 0.13 g, Na 2 SO 3 0.045 g, C 6 h 5 Na 3 o 7 0.22 g, KH 2 PO 4 0.02 g, G-P 0.02 g, CaCl 2 0.07 g, EDTANaFe 0.00024 g, FeSO 4 0.0018 g, ZnSO 4 0.0024 g, MnCl 2 0.00015 g, CoCl 2 0.00005 g, H 3 BO 3 0.00019 g, CuSO 4 0.00014 g, (NH 4 )Mo 7 o 24 0.00003 g, 3-IBA 0.002 g, 6-BA 0.0003 g, Vb 12 0.000025~0.00005 g.
[0056] (2) Add 100 mL of the medium shown above in a 250 mL Erlenmeyer flask, the inoculation ratio is 10% (that is, the volume of the seed liquid accounts for 10% of the culture volume), and the seeds are Haematococcus strain I in the logarithmic growth phase , the inoculation density was 10 3 ~10 4 cells / mL. The culture temperature was (23 ± 1.5) °C, the culture light intensity was 1300-1500 lx, and the culture room was continuously illuminated for static culture. During this period, the culture was shaken several times a day, and three r...
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