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30results about How to "High derivatization efficiency" patented technology

Sample top cavity syringe needle tip deriving method and its uses

The present invention provides a derivatization method and the application thereof of a sample based on the needlepoint in a top empty bottle, and includes the following procedures: 1, using a minim injector to suck the derivatization reagent and insert the needlepoint into the empty top of a top empty battle in which the sample is preset. 2, making the derivatization reagent form a liquid drop hanging on the needlepoint of the injector and exposes in the empty top of the sample. 3, after a period of derivatization, sucking back the derivatization reagent, and the derivatization process is finished. 4, taking the injector out conducts analysis by instruments directly according to requirements. The derivatization method can be applied in top air of the tobacco sample, or acid ingredient in cigarette smoke and the derivatization of aldehyde ketone ingredient. The present invention has the advantages of simple operation, high efficient derivatization, and lower cost; a trace of derivatization reagent can meet the derivatization purpose of the analysis requirements without memory effect and discrimination effect of the analyzed object. Simultaneously, the present invention combines the liquid phase extraction technology and the derivatization means, improves the sensitivity by the enrichment function of the liquid phase extraction, reduces the dosage of the extraction solvent, and shortens the preparation time.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Novel method for detecting etimicin sulfate

The invention relates to a method for detecting the content of an etimicin sulfate raw material and preparation of the etimicin sulfate raw material and relevant substances. The method adopts precolumn derivatization, the derivatization condition comprises taking 4ml sample with appropriate concentration, adding 1ml O phthalic aldehyde (OPA) solution into the sample, diluting the mixed solution with isopropyl alcohol to 10ml, heating the mixed solution at 30-60 DEG C in water bath away from light, and obtaining derivatization solution. The novel method for detecting etimicin sulfate adopts a high efficiency liquid chromatograph, the chromatographic condition is that an octadecylsilane chemically bonded silica filler chromatographic column has particle size ranging from 4-10mum, column specification is 150-250mmX4.6-8.0mm; flowing phase is divided into organic phase and aqueous phase, wherein the volume rate of the organic phase to the aqueous phase is 55-75:45-25, the organic phase is methyl alcohol or acetonitrile, the aqueous phase is glacial acetic acid containing 0.01M-0.03M ion pair reagent sodium heptanesulfonate, potential of hydrogen (PH) range is adjusted by ammonia water or ammonium acetate; flow speed is 0.5-1.0ml / min; sample feeding amount is 10-20mul; a detector is a conventional ultraviolet-visible light detector, and detection wavelength is from 330-350nm. The novel method for detecting etimicin sulfate adopts the conventional detector and the high efficiency liquid chromatograph, can achieve on-line detection of the content of the etimicin sulfate, the preparation of the etimicin sulfate and the relevant substances, and is simple, convenient, feasible, accurate and reliable.
Owner:JIANGXI JEMINCARE GRP CO LTD +1

Aflatoxin and sulfanilamide drug light derivatization device

The invention relates to an flatoxin and sulfanilamide drug light derivatization device. An array ultraviolet LED is taken as an excitation light source, and an excitation wavelength is between 280 nmand 380 nm. A fine internal-diameter pipeline which allows ultraviolet light to pass is taken as a derivatization reaction tank, the derivatization reaction tubes are arranged in a linear mode, the internal diameter of the derivatization reaction tube is 0.15-0.5 millimeters, a length is 0.5-12 meters, and the volume of the derivatization reaction tank is 100-1000 [mu]L. The fluorescence intensity of flatoxin B1 is increased by 6.5 times by the light derivatization device, and the light derivatization device is suitable for requirements of derivatization after high performance liquid chromatography columns and flow injection analysis derivatization.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method for simultaneously detecting 14 sour components in main stream smoke obtained by heating noncombustible cigarettes

The present invention discloses a method for simultaneously detecting 14 sour components in main stream smoke obtained by heating noncombustible cigarettes. The method comprises following steps: 1) gathering main stream smoke particulate substances obtained by heating the noncombustible cigarettes by using a Cambridge filter piece, installing the Cambridge filter piece in an extraction device, adding an acetone solution, adding an inner standard solution, performing vibration extraction, adding a derivatization reagent into supernate, and heating to obtain smoke samples; 2) detecting the smoke samples by GC-MS, and detecting the contents of the 14 sour components in the main stream smoke obtained by heating the noncombustible cigarettes by using an inter-standard quantitative method. The method adopts a solvent extraction-gas chromatographic mass spectrometry and can be used for simultaneously detecting the contents of the 14 sour components in the main stream smoke obtained by heating the noncombustible cigarettes. The method has the advantages of being fast in detection, good in selectivity, and high in precision, and is particularly suitable for detecting the contents of the sour components in the smoke obtained by heating the noncombustible cigarettes.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Method for separating and determining 1,2-propylene glycol enantiomers by using gas chromatography

The invention discloses a method for separating and determining 1,2-propylene glycol, bulk drugs containing 1,2-propylene glycol and 1,2-propylene glycol enantiomer impurities in preparations of the bulk drugs by using precolumn derivatization gas chromatography. The method employs an aldehyde ketone compound as a derivative reagent, carries out precolumn derivatization on the aldehyde ketone compound and 1,2-propylene glycol in the presence of a catalyst and a water reducer and determines trace enantiomer impurities by using gas chromatography. The method provided by the invention overcomes the defect of incapability of accurate quantification of 1,2-propylene glycol enantiomer impurities due to serious trailing of peaks during frequently-used chiral gas chromatographic column separation and guarantees that the quality of 1,2-propylene glycol, the bulk drugs containing 1,2-propylene glycol and the preparations thereof is controllable.
Owner:CHONGQING PHARMA RES INST

Aflatoxin optical derivatization device integrated fluorescence detector

The invention relates to an aflatoxin optical derivatization device integrated fluorescence detector. According to the invention, an optical derivatization device and a fluorescence detector are integrated into a whole, and a derivatization reaction pool is a fluorescence detection pool at the same time, so a post-column volume can be effectively reduced by a half; peak broadening is reduced; thedegree of separation is increased; and sensitivity is improved. The aflatoxin optical derivatization device integrated fluorescence detector provided by the invention uses a high-power ultraviolet LEDas a derivatization light source and an excitation light source and a quartz tube with an internal diameter of 1 to 4 mm as a derivatization pool and a detection pool, adopts an orthogonal fluorescence collection optical path with an included angle of 20 to 70 degrees, can achieve the derivatization efficiency of a traditional optical derivatization device with a derivatization pool with a volumeof 1000 [mu]L by adopting a derivatization pool with a volume of 13 [mu]L, greatly reduces band broadening, has shorter peak appearance time, saves the time of analysis, is simpler and more practical, and is greatly reduced in cost and volume.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method for homogeneously derivatizing plant fiber raw material by taking ionic liquid as medium

InactiveCN102504274AHigh derivatization efficiencyEasy to dissolve and recycleOrganic chemistryFiberDerivatization
The invention relates to a method for homogeneously derivatizing a plant fiber raw material by taking an ionic liquid as a medium. The method comprises the following steps of: (1) adding the plant fiber raw material into the ionic liquid; (2) dissolving at the temperature of 80-140 DEG C for 0.5-48 hours to obtain a reaction mixed solution; (3) leaching and separating the reaction mixed solution with a stainless steel filer screen; (4) adding a derivatization reagent into a filtrate, and undergoing an acylation reaction at the temperature of 70-130 DEG C for 1-10 hours; (5) after reacting, adding a precipitant into the mixed solution to separate a product out, filtering and drying to obtain a plant fiber derivatized product; and (6) distilling the filtrate under reducing pressure and recovering to obtain the ionic liquid. The method disclosed by the invention is simple and practicable, and the obtained plant fiber has a high derivatization degree.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI

Method for detecting hydrazine hydrate in medicine

ActiveCN113295805AComply with testing regulationsImprove linearityComponent separationFluid phasePharmaceutical drug
The invention belongs to the technical field of medicine detection, and particularly discloses a method for detecting hydrazine hydrate in a medicine. The detection method provided by the invention has excellent linearity, detection limit, specificity and accuracy, is good in applicability and reliability, and accords with drug detection regulations; in addition, appropriate dosage and reaction conditions of the derivatization reagent are tested, so that the derivatization efficiency and the stability of a derivative product are improved; parameters of liquid phase and mass spectrum conditions are determined, and the detection sensitivity of derivative products and the separation degree of interference peaks are improved.
Owner:广州国标检验检测有限公司

Top cavity syringe needle tip deriving method for sample and its uses

The present invention provides a derivatization method and the application thereof of a sample based on the needlepoint in a top empty bottle, and includes the following procedures: 1, using a minim injector to suck the derivatization reagent and insert the needlepoint into the empty top of a top empty battle in which the sample is preset. 2, making the derivatization reagent form a liquid drop hanging on the needlepoint of the injector and exposes in the empty top of the sample. 3, after a period of derivatization, sucking back the derivatization reagent, and the derivatization process is finished. 4, taking the injector out conducts analysis by instruments directly according to requirements. The derivatization method can be applied in top air of the tobacco sample, or acid ingredient in cigarette smoke and the derivatization of aldehyde ketone ingredient. The present invention has the advantages of simple operation, high efficient derivatization, and lower cost; a trace of derivatization reagent can meet the derivatization purpose of the analysis requirements without memory effect and discrimination effect of the analyzed object. Simultaneously, the present invention combines the liquid phase extraction technology and the derivatization means, improves the sensitivity by the enrichment function of the liquid phase extraction, reduces the dosage of the extraction solvent, and shortens the preparation time.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Detection and analysis method for trace triptolide in biological sample

The invention belongs to the field of analytical chemistry and in particular relates to a detection and analysis method for the content of trace triptolide in a biological sample. The method specifically comprises the following steps: carrying out stable isotopic labeling derivatization reaction on the triptolide and a derivatization reagent d0- / d3-3-N-methyl-2'-carboxyrhodamine 6G; after filtering a derived product, which is obtained by specifically extracting through a magnetic molecularly imprinted polymer, through a filtering membrane, detecting by utilizing an ultra performance liquid chromatography triple quadrupole tandem mass spectrometry analysis system. According to the detection and analysis method, the trace triptolide is labeled and derived by utilizing a stable isotopic labeling derivatization reagent with permanent positive charge d0- / d3-MCR6G; reaction is moderate and rapid and the efficiency is high; the chromatographic separation degree and the mass spectrum ionization efficiency of an analyte are remarkably improved. The magnetic molecularly imprinted polymer provided by the invention is used for selectively identifying and enriching a target object in a complicated sample and reducing matrix interference; the magnetic molecularly imprinted polymer has the advantages of simplicity in preparation, good stability and high selectivity and recycling rate.
Owner:QUFU NORMAL UNIV +1

A detection and analysis method of trace triptolide in a biological sample

The invention belongs to the field of analytical chemistry and in particular relates to a detection and analysis method for the content of trace triptolide in a biological sample. The method specifically comprises the following steps: carrying out stable isotopic labeling derivatization reaction on the triptolide and a derivatization reagent d0- / d3-3-N-methyl-2'-carboxyrhodamine 6G; after filtering a derived product, which is obtained by specifically extracting through a magnetic molecularly imprinted polymer, through a filtering membrane, detecting by utilizing an ultra performance liquid chromatography triple quadrupole tandem mass spectrometry analysis system. According to the detection and analysis method, the trace triptolide is labeled and derived by utilizing a stable isotopic labeling derivatization reagent with permanent positive charge d0- / d3-MCR6G; reaction is moderate and rapid and the efficiency is high; the chromatographic separation degree and the mass spectrum ionization efficiency of an analyte are remarkably improved. The magnetic molecularly imprinted polymer provided by the invention is used for selectively identifying and enriching a target object in a complicated sample and reducing matrix interference; the magnetic molecularly imprinted polymer has the advantages of simplicity in preparation, good stability and high selectivity and recycling rate.
Owner:QUFU NORMAL UNIV +1

A chip-based photoderivatizer for aflatoxins and sulfonamides

The present invention discloses a chip type light derivatizer for aflatoxins and sulfonamides. An ultraviolet light-emitting diode (LED) having a center wavelength between 280 and 380 nm as an excitation light source, high-transparent quartz, ultraviolet-transmitting glass, PDMS (Polydimethylsiloxane) or perfluoroethylene propylene copolymer (FEP) is used as a chip substrate, a rectangular or trapezoidal groove path having a width of 10 to 500 mu m, a depth of 10 to 260 mu m, and a length of 5 to 500 mm is fabricated on the chip substrate as a derivation reaction tank, the groove path is in linear or comb-like or sine-wave shaped arrangement, the volume of the derivation reaction tank is 5nL to 75mu L, and is only 1 / 13 to 1 / 100000 of the volume of the derivation reaction tank of a conventional derivatizer. The chip type light derivatizer improves the fluorescence intensity of aflatoxin B1 by 6.5 times, and is suitable for post-column light derivation of chip capillary electrophoresis,chip microfluidic analysis, capillary liquid chromatography, microcolumn liquid chromatography, ultrahigh pressure liquid phase chromatography and normal liquid chromatography, and micro flow injection analysis / small flow injection analysis.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Chip type optical derivatization device integrated aflatoxin fluorescence detector

The invention provides a chip type optical derivatization device integrated aflatoxin fluorescence detector. According to the invention, an optical derivatization device and a fluorescence detector are integrated into a whole; a chip type derivatization reaction pool is a fluorescence detection pool at the same time; an optical derivatization pool and a fluorescence detection pool are implementedon a chip of an ultraviolet-permeable material; a high-power ultraviolet LED is simultaneously used as an optical derivatization light source and a fluorescence excitation light source; the LED is perpendicular to an optical derivatization / detection pool; the angle between a photoelectric detector and the LED is 20 to 70 degrees; and when the chip type optical derivatization device provided by theinvention has a pool volume of 7.5 [mu]L, the derivatization efficiency of the chip type optical derivatization device is close to the derivatization efficiency of a traditional optical derivatization device with a pool volume of 1000 [mu]L. Thus, the post-column volume after chromatographic column separation is greatly reduced; peak broadening is significantly reduced; the degree of separation is improved; the lower limit of detection is reduced; the overall performance and reliability of the chip type optical derivatization device integrated aflatoxin fluorescence detector are improved; andthe volume and cost of the chip type optical derivatization device integrated aflatoxin fluorescence detector are greatly reduced at the same time.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI

Method for detecting vitamin D metabolite

The invention provides a method for detecting a vitamin D metabolite. The method comprises the following steps that: an analyte, namely the vitamin D metabolite, in a serum sample, is extracted by using an organic solvent; and a derivative reagent 4-phenylurazole and iodobenzene diacetate are added into a blow-dried extraction residue, so that Diels-Alder reaction can be performed. According to the method, the vitamin D metabolite is detected by using a liquid chromatography-tandem mass spectrometry method. The method has the advantages of low cost, short reaction time, high sensitivity and high interference resistance.
Owner:上海医药临床研究中心 +1

Simultaneous determination of 14 sour components in mainstream smoke of heat-not-burn cigarettes

The present invention discloses a method for simultaneously detecting 14 sour components in main stream smoke obtained by heating noncombustible cigarettes. The method comprises following steps: 1) gathering main stream smoke particulate substances obtained by heating the noncombustible cigarettes by using a Cambridge filter piece, installing the Cambridge filter piece in an extraction device, adding an acetone solution, adding an inner standard solution, performing vibration extraction, adding a derivatization reagent into supernate, and heating to obtain smoke samples; 2) detecting the smoke samples by GC-MS, and detecting the contents of the 14 sour components in the main stream smoke obtained by heating the noncombustible cigarettes by using an inter-standard quantitative method. The method adopts a solvent extraction-gas chromatographic mass spectrometry and can be used for simultaneously detecting the contents of the 14 sour components in the main stream smoke obtained by heating the noncombustible cigarettes. The method has the advantages of being fast in detection, good in selectivity, and high in precision, and is particularly suitable for detecting the contents of the sour components in the smoke obtained by heating the noncombustible cigarettes.
Owner:ZHENGZHOU TOBACCO RES INST OF CNTC

Novel method for detecting etimicin sulfate

The invention relates to a method for detecting the content of an etimicin sulfate raw material and preparation of the etimicin sulfate raw material and relevant substances. The method adopts precolumn derivatization, the derivatization condition comprises taking 4ml sample with appropriate concentration, adding 1ml O phthalic aldehyde (OPA) solution into the sample, diluting the mixed solution with isopropyl alcohol to 10ml, heating the mixed solution at 30-60 DEG C in water bath away from light, and obtaining derivatization solution. The novel method for detecting etimicin sulfate adopts a high efficiency liquid chromatograph, the chromatographic condition is that an octadecylsilane chemically bonded silica filler chromatographic column has particle size ranging from 4-10mum, column specification is 150-250mmX4.6-8.0mm; flowing phase is divided into organic phase and aqueous phase, wherein the volume rate of the organic phase to the aqueous phase is 55-75:45-25, the organic phase is methyl alcohol or acetonitrile, the aqueous phase is glacial acetic acid containing 0.01M-0.03M ion pair reagent sodium heptanesulfonate, potential of hydrogen (PH) range is adjusted by ammonia water or ammonium acetate; flow speed is 0.5-1.0ml / min; sample feeding amount is 10-20mul; a detector is a conventional ultraviolet-visible light detector, and detection wavelength is from 330-350nm. The novel method for detecting etimicin sulfate adopts the conventional detector and the high efficiency liquid chromatograph, can achieve on-line detection of the content of the etimicin sulfate, the preparation of the etimicin sulfate and the relevant substances, and is simple, convenient, feasible, accurate and reliable.
Owner:JIANGXI JEMINCARE GRP CO LTD +1

Minitype optical derivatization device for aflatoxin and sulfonamides

ActiveCN109387576AReduce Derivative Pool VolumeReduce stretchingComponent separationFluorescence/phosphorescencePhysicsPost column derivatization
The invention provides a minitype optical derivatization device for aflatoxin and sulfonamides. The minitype optical derivatization device comprises an ultraviolet LED light source with an excitationwavelength in a range of 280 to 370 nm, and adopts an ultraviolet-permeable pipe with a small internal diameter as a derivation reaction pool, wherein a derivatization reaction pipe is spirally woundon the inner wall of alight-reflective bowl in a single layer manner, and has an internal diameter of 0.10 to 0.35 mm and a length of 17 to 30 cm; and the derivation reaction pool has a volume of 7 to15 [mu]L. The optical derivatization device provided by the invention increases the fluorescence intensity of aflatoxin B1 by 5 times; and the optical derivatization device meets the requirements ofliquid chromatography post-column derivatization and flow injection analysis derivatization, and is used for analyzing trace aflatoxin and sulfonamides in a sample through combined application of high-performance liquid chromatography (HPLC) and ultra-high-pressure liquid chromatography (UPLC).
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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