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A Fluorescence Detector Integrated with Aflatoxin Photoderivatizer

A technology integrating Aspergillus flavus and fluorescence detectors, applied in the fields of photochemical derivatization and fluorescence detection, can solve problems such as reducing sensitivity, increasing peak broadening, and reducing resolution, and achieves the effects of improving sensitivity, reducing peak broadening, and increasing resolution.

Active Publication Date: 2021-06-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the separation and connection of the two will eventually increase a part of the post-column volume, which will slightly increase peak broadening, reduce resolution and reduce sensitivity.

Method used

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  • A Fluorescence Detector Integrated with Aflatoxin Photoderivatizer
  • A Fluorescence Detector Integrated with Aflatoxin Photoderivatizer

Examples

Experimental program
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Effect test

Embodiment 1

[0031]A high-performance liquid chromatography (HPLC) integrated aflatoxin light derivatizer fluorescence detector, the excitation light source is a high-power ultraviolet LED with a central wavelength of 365nm, a divergence angle of 60°, and a rated current of 1000mA; the ultraviolet LED light source and the derivatization pool The distance between the derivation / detection cell is 5 mm; the bandwidth of the excitation filter is 35 nm; the derivation / detection cell is a quartz tube with an inner diameter of 2 mm, an outer diameter of 4 mm, and an effective length of 4 mm; the volume of the derivation / reaction cell is 13 μL; The distance between the mirror and the derivation cell / detection cell is 4 mm; the diameter of the fluorescence collection lens is 8 mm, and the focal length is 8.5 mm; the central wavelength of the emission filter is 440 nm, and the bandwidth is 30 nm; the photodetector device is the AccuOpt 2000 photoelectric amplifier developed by Dalian Institute of Chem...

Embodiment 2

[0036] For the fluorescence detector integrated with the aflatoxin light derivatizer described in Example 1, the quartz tube was replaced with an inner diameter of 3 mm and an outer diameter of 5 mm, and was combined with HPLC to analyze and detect four kinds of sulfa drugs. The excitation wavelength of RF-20A is 230nm, and the emission wavelength is 400nm.

[0037] Experimental results: 5ppb sulfadiazine (SDZ) was injected, and RF-20A was not detected; the fluorescence detector connected to the integrated aflatoxin light derivatizer of the present invention, 5ppb sulfadiazine (SDZ) could be detected, and the detection signal-to-noise ratio was 15 .

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Abstract

The invention discloses a fluorescence detector integrated with aflatoxin light derivatizer, which integrates the light derivatizer and the fluorescence detector, and the derivatization reaction pool is also the fluorescence detection pool. This effectively cuts the "half" post-column volume, reduces peak broadening, increases resolution, and improves sensitivity. High-power ultraviolet LED is used as the derivative light source and excitation light source at the same time; the quartz tube with an inner diameter of 1-4mm is used as the derivative cell and the detection cell at the same time; the orthogonal, 20-70° fluorescence collection light path is adopted; the volume of the 13μL derivative cell can obtain the traditional light derivative The derivatization efficiency of the 1000μL cell volume of the analyzer greatly reduces the band broadening, the peak time is shorter, the analysis time is saved, and it is simpler, more practical, and the cost and volume are greatly reduced.

Description

technical field [0001] The patent of the present invention relates to the fields of photochemical derivatization and fluorescence detection of high performance liquid chromatography (HPLC), flow injection analysis (FIA), and more specifically, relates to a fluorescence detector integrating aflatoxin light derivatization device. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins and sulfonamides by high-performance liquid chromatography (HPLC)-fluorescence detection, and are used to selectively enhance the fluorescence of aflatoxins B1, G1 and sulfonamides such as sulfadiazine, etc. strength. Among the many aflatoxin detection methods, the earliest and most widely used method is the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method, such as the national standard GB / T 18979-2003 and GB / T 23212- 2008. [0003] Aflatoxin B1 and G1 originally have strong fluorescence, but after they contact w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/74G01N30/02G01N21/64
CPCG01N21/643G01N30/02G01N30/74
Inventor 耿旭辉关亚风高岩宁海静
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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