Minitype optical derivatization device for aflatoxin and sulfonamides

Abstract

The invention provides a minitype optical derivatization device for aflatoxin and sulfonamides. The minitype optical derivatization device comprises an ultraviolet LED light source with an excitationwavelength in a range of 280 to 370 nm, and adopts an ultraviolet-permeable pipe with a small internal diameter as a derivation reaction pool, wherein a derivatization reaction pipe is spirally woundon the inner wall of alight-reflective bowl in a single layer manner, and has an internal diameter of 0.10 to 0.35 mm and a length of 17 to 30 cm; and the derivation reaction pool has a volume of 7 to15 [mu]L. The optical derivatization device provided by the invention increases the fluorescence intensity of aflatoxin B1 by 5 times; and the optical derivatization device meets the requirements ofliquid chromatography post-column derivatization and flow injection analysis derivatization, and is used for analyzing trace aflatoxin and sulfonamides in a sample through combined application of high-performance liquid chromatography (HPLC) and ultra-high-pressure liquid chromatography (UPLC).

Description

technical field [0001] The patent of the present invention relates to the field of optical detection of high performance liquid chromatography (HPLC) and flow injection analysis (FIA), more specifically, relates to a light derivatizer for aflatoxin and sulfa drugs. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins by high-performance liquid chromatography (HPLC)-fluorescence detection, such as national standards GB / T 18979-2003 and GB / T 23212-2008, and the 2015 Chinese Pharmacopoeia that photoderivatization The instrument is included in the chapter on the determination of aflatoxin, which is used to assist in the determination of trace aflatoxin in medicinal materials, decoction pieces and preparations. Aflatoxin (AFTS) is a molecular mycotoxin, which is known as the strongest natural carcinogen. In 1993, aflatoxin was classified as a class I carcinogen by the Cancer Research Institute of the World Health Organization...

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Problems solved by technology

But at present, whether it is a photochemical derivatizer for scientific research (Fan Haidong, Wang Lingfeng, Zhao Dongji, Self-made Photochemical Derivatization Reactor-High Performance Liquid Chromatography for the Determination of Aflatoxin B1 in Soybean Oil, Physical and Chemical Testing-Chemical Volume, 2013, 49, 999- 1001) or a commercial photochemical derivatizer (http: / / www.josvok.com / product_show.php?id=57), (http: / / www.18show.cn / zt410303 / Product_10226818.html), the excitation light source They are all 254nm low-pressure mercury lamps with a lifespan of only 2000-3000 hours. The emitted light intensity gradually attenuates with the increase of the lighting time of the light source, and the light derivative efficiency decreases accordingly, resulting in a continuous decrease in the light derivative efficiency during the life cycle of the lamp; The reaction tube used in the derivatizer is a highly transparent FEP tube with an inner diameter of 0.4-0.5 mm, a length of 10-12 meters, and a volume of the derivatization pool of 1 mL; a densely woven ring structure is used to form a rectangular net, which makes the flow to obtain good mixing of the processed liquid while minimizing band broadening

But the net of such a loop structure is compiled by hand, which is time-consuming and laborious; the price of a commercial photochemical derivatizer is between 30,000 and 40,000 yuan.

In short, the photochemical derivatives in the prior art have many problems such as short light source life, complicated production, large volume, and high price.

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