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A Fluorescence Detector of Aflatoxin with Integrated Chip-type Optical Derivatizer

An aflatoxin and fluorescence detector technology, which is applied in the fields of photochemical derivatization and fluorescence detection, can solve the problems of reducing sensitivity, increasing peak broadening, and reducing resolution, and achieves the effects of improving sensitivity, reducing peak broadening, and increasing resolution.

Active Publication Date: 2021-11-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the separation and connection of the two will eventually increase a part of the post-column volume, which will slightly increase peak broadening, reduce resolution and reduce sensitivity.

Method used

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  • A Fluorescence Detector of Aflatoxin with Integrated Chip-type Optical Derivatizer
  • A Fluorescence Detector of Aflatoxin with Integrated Chip-type Optical Derivatizer
  • A Fluorescence Detector of Aflatoxin with Integrated Chip-type Optical Derivatizer

Examples

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Effect test

Embodiment 1

[0031] A kind of aflatoxin fluorescence detector with integrated chip light derivatizer, the excitation light source is a high-power central wavelength 365nm ultraviolet LED, the divergence angle is 60°, and the rated current is 1000mA; the distance between the ultraviolet LED light source and the derivation cell / detection cell 3mm; the bandwidth of the excitation filter is 35nm; the chip substrate is made of highly transparent quartz glass, on which there are chip-derived reaction channels arranged in a comb shape. The channel is a rectangular channel with a width of 150 μm, a depth of 200 μm, and a length of 250 mm. 7.5μL; a small mirror is installed under the chip derivation / detection cell, the distance between the mirror and the chip derivation / detection cell is 3mm; the fluorescence collection lens is located between the emission filter and the photoelectric detection device, with a diameter of 8mm and a focal length of 8.5mm ; The central wavelength of the emission filter...

Embodiment 2

[0036] The aflatoxin fluorescence detector integrated with the chip-type light derivatizer described in Example 1 was used to analyze and detect four kinds of sulfa drugs. RF-20A has an excitation wavelength of 230nm and an emission wavelength of 400nm.

[0037] Experimental result: sample injection 5ppb sulfadiazine (SDZ), RF-20A is not detected; Connect the aflatoxin fluorescence detector of integrated chip type light derivatization device of the present invention, 5ppb sulfadiazine (SDZ) can be detected, detect signal noise than 15.

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Abstract

The invention discloses an aflatoxin fluorescence detector integrated with a chip-type light derivatizer, which integrates the light derivatizer and the fluorescence detector, and the chip-type derivatization reaction pool is also a fluorescence detection pool. The light derivation cell and the fluorescence detection cell are implemented on a chip of UV-transparent material. The high-power ultraviolet LED used is used as a light derivative light source and a fluorescence excitation light source at the same time. The LED is perpendicular to the light derivation cell / detection cell, and the included angle between the photoelectric detection device and the LED is 20-70°. When the invented chip light derivatizer has a cell volume of 7.5 μL, its derivatization efficiency is close to that of the traditional light derivatizer with a cell volume of 1000 μL. The invention not only greatly reduces the post-column volume after chromatographic column separation, significantly reduces peak broadening, improves resolution, and lowers the lower limit of detection, but also improves the overall performance and reliability of the instrument, and at the same time greatly reduces the volume and cost of the instrument.

Description

technical field [0001] The patent of the present invention relates to the fields of photochemical derivatization and fluorescence detection of high performance liquid chromatography (HPLC), flow injection analysis (FIA), and more specifically, relates to a kind of aflatoxin fluorescence detector integrated with a chip-type light derivatization device. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins and sulfonamides by high-performance liquid chromatography (HPLC)-fluorescence detection, and are used to selectively enhance the fluorescence of aflatoxins B1, G1 and sulfonamides such as sulfadiazine, etc. strength. Among the many aflatoxin detection methods, the earliest and most widely used method is the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method, such as the national standard GB / T 18979-2003 and GB / T 23212- 2008. [0003] Aflatoxin B1 and G1 originally have strong fluorescence, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/74
CPCG01N30/74
Inventor 耿旭辉关亚风宁海静冯春波
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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