The invention discloses a high performance liquid chromatography detection method for pyrazine compounds in Baijiu. According to the method, a high performance liquid chromatography-fluorescence detector (HPLC-FLD) method is adopted to perform qualitative and quantitative analysis on the pyrazine compounds in Baijiu, and 2-methylpyrazine, 2, 3-dimethylpyrazine, 2, 5-dimethylpyrazine, 2, 6-dimethylpyrazine, 2, 3, 5-trimethyl pyrazine, 2-ethyl-6-methylpyrazine, 2, 3, 5, 6-tetramethylpyrazine and 2, 3-dimethyl-5-ethylpyrazine can be measured at the same time. With the adoption of the method, 3 types of isomeride can be separated without chemical derivation, chiral columns or adding chiral reagents, is high in sensitivity, good in accuracy and repeatability, simple to operate, and low in cost.