Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

HPLC-FLD pre-column derivatization method for simultaneously determining aflatoxins B1, B2, G1 and G2 in tobacco

An aflatoxin and derivatization technology, which is applied in the field of HPLC-FLD pre-column derivatization, can solve the problems of inability to obtain derivatization effect, weakened derivatization effect, poor derivatization effect, etc., and achieves good linear relationship and low detection limit. , good separation effect

Inactive Publication Date: 2015-01-14
中国烟草总公司湖北省公司
View PDF6 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experiments have found that after derivatization, blown dry with nitrogen, the derivatization effect is poor, and it is almost impossible to obtain the derivatization effect
This may be related to AFB 2a and AFG 2a It is related to the low stability of the molecule. In the process of drying with nitrogen, the hemiacetal obtained by the derivatization reaction decomposes again, which weakens the derivatization effect, resulting in the inability to aflatoxin B. 1 , B 2 , G 1 and G 2 Simultaneous determination of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • HPLC-FLD pre-column derivatization method for simultaneously determining aflatoxins B1, B2, G1 and G2 in tobacco
  • HPLC-FLD pre-column derivatization method for simultaneously determining aflatoxins B1, B2, G1 and G2 in tobacco
  • HPLC-FLD pre-column derivatization method for simultaneously determining aflatoxins B1, B2, G1 and G2 in tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] The materials, reagents and instruments used in this embodiment are as follows:

[0021] Dionex Summit P680A high performance liquid chromatograph (with autosampler, column thermostat and DAD detector), AL204 1 / 10,000 electronic balance, pipette (Gilson), 2mL sample vial, pipette tip, Manual solid phase extraction (Supelco), aflatoxin immunoaffinity column (Beacon, USA).

[0022] Methanol (chromatographically pure, CNW), acetonitrile (chromatographically pure, CNW), n-hexane (chromatographically pure, CNW), trifluoroacetic acid, purified water (wahhaha).

[0023] The determination method is as follows:

[0024] Prepare the sample according to YC / T 31, weigh 2.00g of smoke powder sample, put it in a 30mL plastic bottle with a cover, extract it in an ultrasonic instrument with 20mL methanol solution with a volume fraction of 80% in a water bath for 10min, and shake it every two minutes; extract Filter after completion; take 5mL of filtrate and add 20mL of pure water to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Excitation wavelengthaaaaaaaaaa
Emission wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses an HPLC-FLD pre-column derivatization method for simultaneously determining aflatoxins B1, B2, G1 and G2 in tobacco. The HPLC-FLD pre-column derivatization method comprises the following steps: performing water-bath ultrasonic extraction on a tobacco powder sample by use of a methanol solution, filtering, diluting the filtrate and enabling the diluted filtrate to pass through an immunoaffinity column, performing twice drip washing on the immunoaffinity column by use of pure water, next, eluting four aflatoxins enriched on the immunoaffinity column by use of methanol, performing blow-drying on the eluted solution by use of nitrogen, then, adding trifluoroacetic acid and normal hexane for derivatization at the constant temperature being 45 DEG C for 40 minutes, adding a small quantity of methanol solution and carrying out HPLC-FLD analysis on the taken low-layer solution. The HPLC-FLD pre-column derivatization method can be applied to simultaneous determination of the aflatoxins B1, B2, G1 and G2 in tobacco and tobacco products, and is capable of completing determination in 20 minutes; the four targets can be well separated, the linear relation is good and the detection limit is low; besides, the HPLC-FLD pre-column derivatization method has good recovery rate and reproducibility, and is suitable for detecting the aflatoxins in tobacco and tobacco products.

Description

technical field [0001] The invention relates to a detection method of aflatoxin, in particular to a method for simultaneously determining aflatoxin B in tobacco 1 , B 2 , G 1 and G 2 HPLC-FLD pre-column derivatization method. Background technique [0002] Aflatoxin (AFT) is a highly toxic fungal metabolite discovered in the early 1960s. It is mainly produced by Aspergillus flaws and Aspergillus parasiticus after infecting agricultural products. It is one of the strongest known carcinogens and is extremely toxic. In 1993, aflatoxin was classified as a first-class carcinogen by the World Health Organization Cancer Research Agency. [0003] The main aflatoxins are B 1 , B 2 , G 1 , G 2 etc., the structural formula is as follows, wherein aflatoxin B 1 (AFB 1 ) is the most toxic and the most common. [0004] [0005] Aflatoxins widely exist in agricultural products such as rice, peanuts, corn, and cottonseed, and are a huge threat to food safety and international f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88
Inventor 王康李韵肖少红
Owner 中国烟草总公司湖北省公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com