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Chip type optical derivatization device integrated aflatoxin fluorescence detector

A fluorescence detector and integrated Aspergillus flavus technology, applied in the field of photochemical derivatization and fluorescence detection, can solve the problems of reducing sensitivity, reducing resolution, increasing peak broadening, etc., to achieve the goal of saving analysis time, reducing peak broadening, and increasing resolution Effect

Active Publication Date: 2019-02-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the separation and connection of the two will eventually increase a part of the post-column volume, which will slightly increase peak broadening, reduce resolution and reduce sensitivity.

Method used

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  • Chip type optical derivatization device integrated aflatoxin fluorescence detector
  • Chip type optical derivatization device integrated aflatoxin fluorescence detector
  • Chip type optical derivatization device integrated aflatoxin fluorescence detector

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A kind of aflatoxin fluorescence detector with integrated chip light derivatizer, the excitation light source is a high-power central wavelength 365nm ultraviolet LED, the divergence angle is 60°, and the rated current is 1000mA; the distance between the ultraviolet LED light source and the derivation cell / detection cell 3mm; the bandwidth of the excitation filter is 35nm; the chip substrate is made of highly transparent quartz glass, on which there are chip-derived reaction channels arranged in a comb shape. The channel is a rectangular channel with a width of 150 μm, a depth of 200 μm, and a length of 250 mm. 7.5μL; a small mirror is installed under the chip derivation / detection cell, the distance between the mirror and the chip derivation / detection cell is 3mm; the fluorescence collection lens is located between the emission filter and the photoelectric detection device, with a diameter of 8mm and a focal length of 8.5mm ; The central wavelength of the emission filter...

Embodiment 2

[0036] The aflatoxin fluorescence detector integrated with the chip-type light derivatizer described in Example 1 was used to analyze and detect four kinds of sulfa drugs. RF-20A has an excitation wavelength of 230nm and an emission wavelength of 400nm.

[0037] Experimental result: sample injection 5ppb sulfadiazine (SDZ), RF-20A is not detected; Connect the aflatoxin fluorescence detector of integrated chip type light derivatization device of the present invention, 5ppb sulfadiazine (SDZ) can be detected, detect signal noise than 15.

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Abstract

The invention provides a chip type optical derivatization device integrated aflatoxin fluorescence detector. According to the invention, an optical derivatization device and a fluorescence detector are integrated into a whole; a chip type derivatization reaction pool is a fluorescence detection pool at the same time; an optical derivatization pool and a fluorescence detection pool are implementedon a chip of an ultraviolet-permeable material; a high-power ultraviolet LED is simultaneously used as an optical derivatization light source and a fluorescence excitation light source; the LED is perpendicular to an optical derivatization / detection pool; the angle between a photoelectric detector and the LED is 20 to 70 degrees; and when the chip type optical derivatization device provided by theinvention has a pool volume of 7.5 [mu]L, the derivatization efficiency of the chip type optical derivatization device is close to the derivatization efficiency of a traditional optical derivatization device with a pool volume of 1000 [mu]L. Thus, the post-column volume after chromatographic column separation is greatly reduced; peak broadening is significantly reduced; the degree of separation is improved; the lower limit of detection is reduced; the overall performance and reliability of the chip type optical derivatization device integrated aflatoxin fluorescence detector are improved; andthe volume and cost of the chip type optical derivatization device integrated aflatoxin fluorescence detector are greatly reduced at the same time.

Description

technical field [0001] The patent of the present invention relates to the fields of photochemical derivatization and fluorescence detection of high performance liquid chromatography (HPLC), flow injection analysis (FIA), and more specifically, relates to a kind of aflatoxin fluorescence detector integrated with a chip-type light derivatization device. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins and sulfonamides by high-performance liquid chromatography (HPLC)-fluorescence detection, and are used to selectively enhance the fluorescence of aflatoxins B1, G1 and sulfonamides such as sulfadiazine, etc. strength. Among the many aflatoxin detection methods, the earliest and most widely used method is the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method, such as the national standard GB / T 18979-2003 and GB / T 23212- 2008. [0003] Aflatoxin B1 and G1 originally have strong fluorescence, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/74
CPCG01N30/74
Inventor 耿旭辉关亚风宁海静冯春波
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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