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A photoderivatizer for aflatoxins and sulfonamides

A technology of sulfonamides and aflatoxins, applied in the field of optical detection, can solve the problems of complex braiding of derivative tubes, short light source life, and high price, and achieve easy disassembly and replacement of parts, reduced band broadening, and low cost Effect

Active Publication Date: 2020-05-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current light derivatizer has the disadvantages of short life of the light source, complex braiding of the derivative tube, large volume, and high price.

Method used

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  • A photoderivatizer for aflatoxins and sulfonamides
  • A photoderivatizer for aflatoxins and sulfonamides
  • A photoderivatizer for aflatoxins and sulfonamides

Examples

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Effect test

Embodiment 1

[0044] A high-performance liquid chromatography (HPLC) post-column optical derivatizer, the excitation light source is 3 high-power ultraviolet LEDs with a central wavelength of 365nm, the divergence angle is 120°, and the rated current is 500mA; the distance between the ultraviolet LED light source and the derivatization reaction tube is 5mm; The reaction tube is a highly transparent FEP tube with an inner diameter of 0.35mm, an outer diameter of 1.6mm, and a length of 1.5m. The volume of the derivative pool is 144μL, and the distance between the derivative reaction tube and the reflector is 4mm; the reflector is a stainless steel polished plate; the material of the LED substrate and heat sink The material of the derivative frame is metal aluminum; the material of the metal rod is stainless steel. The photochemical derivatizer was connected with AccuOpt FD-1200S fluorescence detector (Dalian Kemei Precision Instrument Co., Ltd.) to evaluate its performance. The liquid chromat...

Embodiment 2

[0049] The photochemical derivatizer as described in Example 1 was combined with Shimadzu RF-20A fluorescence detector to analyze and detect 4 kinds of sulfa drugs. RF-20A has an excitation wavelength of 230nm and an emission wavelength of 400nm.

[0050] Experimental results: 5ppb sulfadiazine (SDZ) was injected, and RF-20A was not detected when the photochemical derivatizer was not connected; after the photochemical derivatizer was connected, 5ppb sulfadiazine (SDZ) could be detected, and the detection signal-to-noise ratio was 15.

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Abstract

The invention relates to an flatoxin and sulfanilamide drug light derivatization device. An array ultraviolet LED is taken as an excitation light source, and an excitation wavelength is between 280 nmand 380 nm. A fine internal-diameter pipeline which allows ultraviolet light to pass is taken as a derivatization reaction tank, the derivatization reaction tubes are arranged in a linear mode, the internal diameter of the derivatization reaction tube is 0.15-0.5 millimeters, a length is 0.5-12 meters, and the volume of the derivatization reaction tank is 100-1000 [mu]L. The fluorescence intensity of flatoxin B1 is increased by 6.5 times by the light derivatization device, and the light derivatization device is suitable for requirements of derivatization after high performance liquid chromatography columns and flow injection analysis derivatization.

Description

technical field [0001] The patent of the present invention relates to the field of optical detection of high performance liquid chromatography (HPLC) and flow injection analysis (FIA), more specifically, relates to a light derivatizer for aflatoxin and sulfa drugs. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins and sulfa drugs by high performance liquid chromatography (HPLC)-fluorescence detection, and are used to selectively enhance the fluorescence intensity of aflatoxin B1, G1 and sulfa drugs such as sulfadiazine . Among the many aflatoxin detection methods, the earliest and most widely used method is the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method, such as the national standard GB / T 18979-2003 and GB / T 23212- 2008. With the development of the times and the advancement of science and technology, the limit values ​​of aflatoxins in various substances in national standards and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N30/06G01N30/74
CPCG01N21/6428G01N30/06G01N30/74
Inventor 关亚风耿旭辉王楠黄晓东
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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