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Aflatoxin optical derivatization device integrated fluorescence detector

A technology integrating Aspergillus flavus and fluorescence detectors, applied in the field of photochemical derivatization and fluorescence detection, can solve the problems of reducing sensitivity, reducing resolution, increasing peak broadening, etc., to achieve the effect of improving sensitivity, reducing peak broadening, and increasing resolution

Active Publication Date: 2019-02-26
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

However, the separation and connection of the two will eventually increase a part of the post-column volume, which will slightly increase peak broadening, reduce resolution and reduce sensitivity.

Method used

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Embodiment 1

[0031]A high-performance liquid chromatography (HPLC) integrated aflatoxin light derivatizer fluorescence detector, the excitation light source is a high-power ultraviolet LED with a central wavelength of 365nm, a divergence angle of 60°, and a rated current of 1000mA; the ultraviolet LED light source and the derivatization pool The distance between the derivation / detection cell is 5 mm; the bandwidth of the excitation filter is 35 nm; the derivation / detection cell is a quartz tube with an inner diameter of 2 mm, an outer diameter of 4 mm, and an effective length of 4 mm; the volume of the derivation / reaction cell is 13 μL; The distance between the mirror and the derivation cell / detection cell is 4 mm; the diameter of the fluorescence collection lens is 8 mm, and the focal length is 8.5 mm; the central wavelength of the emission filter is 440 nm, and the bandwidth is 30 nm; the photodetector device is the AccuOpt 2000 photoelectric amplifier developed by Dalian Institute of Chem...

Embodiment 2

[0036] For the fluorescence detector integrated with the aflatoxin light derivatizer described in Example 1, the quartz tube was replaced with an inner diameter of 3 mm and an outer diameter of 5 mm, and was combined with HPLC to analyze and detect four kinds of sulfa drugs. RF-20A has an excitation wavelength of 230nm and an emission wavelength of 400nm.

[0037] Experimental results: 5ppb sulfadiazine (SDZ) was injected, and RF-20A was not detected; the fluorescence detector connected to the integrated aflatoxin light derivatizer of the present invention, 5ppb sulfadiazine (SDZ) could be detected, and the detection signal-to-noise ratio was 15 .

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Abstract

The invention relates to an aflatoxin optical derivatization device integrated fluorescence detector. According to the invention, an optical derivatization device and a fluorescence detector are integrated into a whole, and a derivatization reaction pool is a fluorescence detection pool at the same time, so a post-column volume can be effectively reduced by a half; peak broadening is reduced; thedegree of separation is increased; and sensitivity is improved. The aflatoxin optical derivatization device integrated fluorescence detector provided by the invention uses a high-power ultraviolet LEDas a derivatization light source and an excitation light source and a quartz tube with an internal diameter of 1 to 4 mm as a derivatization pool and a detection pool, adopts an orthogonal fluorescence collection optical path with an included angle of 20 to 70 degrees, can achieve the derivatization efficiency of a traditional optical derivatization device with a derivatization pool with a volumeof 1000 [mu]L by adopting a derivatization pool with a volume of 13 [mu]L, greatly reduces band broadening, has shorter peak appearance time, saves the time of analysis, is simpler and more practical, and is greatly reduced in cost and volume.

Description

technical field [0001] The patent of the present invention relates to the fields of photochemical derivatization and fluorescence detection of high performance liquid chromatography (HPLC), flow injection analysis (FIA), and more specifically, relates to a fluorescence detector integrating aflatoxin light derivatization device. Background technique [0002] Photoderivatizers are widely used in the quantitative detection of aflatoxins and sulfonamides by high-performance liquid chromatography (HPLC)-fluorescence detection, and are used to selectively enhance the fluorescence of aflatoxins B1, G1 and sulfonamides such as sulfadiazine, etc. strength. Among the many aflatoxin detection methods, the earliest and most widely used method is the high performance liquid chromatography-fluorescence detection (HPLC-FLD) method, such as the national standard GB / T 18979-2003 and GB / T 23212- 2008. [0003] Aflatoxin B1 and G1 originally have strong fluorescence, but after they contact w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/74G01N30/02G01N21/64
CPCG01N21/643G01N30/02G01N30/74
Inventor 耿旭辉关亚风高岩宁海静
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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