35results about How to "Good dose-response relationship" patented technology

The invention discloses a method for measuring superoxide anion radicals in tape grass leaves, belonging to the measuring field of the measuring of superoxide anion radicals. The method comprises the following steps of: firstly, collecting and cleaning the tip parts of fully exposed small and tender tape grass leaves; carrying out Tiron captureing on the Tiron of radicals in a closed operation box in a closed nitrogen environment; and finally measuring by using an EPR (Electron Paramagnetic Resonance) technology. The invention has the advantages of simpler operation and easy grasping, and can measure the superoxide anion radicals of the tape grass leaves in a very short time;, find that and the measured radicals are found to be the superoxide anion radicals according to the superfine structure constant and, the spectrogram shape analysis of an EPR spectrogram and the combination of the remarkable decreases of the radical strength of the leaves pretreated by SOD (Super Oxide Dismutase), and therefore the invention is the most direct, accurate and effective method for measuring the superoxide anion radicals. Superoxide anions are sensitive to pollutants and have a favorable dosage effect relation. The invention is also suitable for other aquatic plant and terrestrial plant species and can be suitable for measuring the radicals of the plant root tissue.

The present invention belongs to the field of natural medicines and discloses a compound medicine composition with anti lymphoma activity containing piperlongumine and houttugnin and use thereof. Active ingredients of the compound medicine composition comprise the piperlongumine and the houttugnin in a molar ratio of 1:1 to 1:20. Through combination of the piperlongumine and the houttugnin, the compound medicine composition has a strong synergistic treatment effect on lymphoma.

The invention discloses application of nobiletin, and particularly discloses application of nobiletin in preparation of health products or medicines for preventing and / or treating oral cancer. Through the anti-proliferation effects of monomeric compounds, namely hesperetin, naringenin and nobiletin, in rutaceae citrus fruits and peels on human oral epidermoid carcinoma cells, the experiments show that the hesperetin, naringenin and nobiletin have an obvious effect on inhibiting proliferation of human oral epidermoid carcinoma cells, and the effect of the nobiletin on inhibiting oral cancer cells is most obvious, so that the nobiletin can be used for preparing health products and medicines with effects on preventing and treating oral cancer and is suitable for large-scale popularization and application.

The invention belongs to the field of natural medicine, and discloses a compound medicine composition with a lung cancer resistant effect and application of the compound medicine composition. Active ingredients of the compound medicine composition consist of piperlongumine and rheum emodin in a molar ratio of (1:1) to (1:10). By combining the piperlongumine and rheum emodin, a synergic effect for treating the lung cancer is relatively good.

This invention concerns the method of extracting salvianolic acid A from Chinese crude drug: danshen root, and the quality control methods and drug combinations, and the application of this drug. It can be used in the preparation of the prevention drugs for cardiovascular disease, liver damage, liver fibrosis, pulmonary fibrosis and other.

The invention discloses an application of bufotalin std. in preparing a medicament for treating lung tumors. Proved by in vitro anti-tumor experiments of the bufotalin std., the concentration of medicaments of the animal lungs is higher than the concentration of effecting medicaments, and the bufotalin std. has stronger activity inhibition effect on human non-small cell lung cancer and other lung tumor cell stains, wherein the IC50 is 15-200ng / ml, and the bufotalin std. has good dose-response relationship. Shown as experimental results, the tumor growth inhibition effect of the bufotalin std. is superior to that of medicaments clinically applied at present, and the bufotalin std. has great development and application prospects.

The present invention is compound total arasaponin-puerain preparation, and features that total arasaponin in 21-82 wt% and puerain in 18-79 wt% as well as proper amount of medicinal or food supplementary material are produced into medicine or food preparation. The compound total arasaponin-puerain preparation has the synergistic effects of regulating blood function, treating cardiac and cerebral vascular diseases, lowering serum total cholesterol and triglyceride, reducing blood viscosity, improving micro circulation, etc.

The water cress extract contains active components including quercetin, isorhamnetin, general chlorogenic acid. The preparation process of the water cress extract includes the following steps: refluxextractio of water cress material in solvent, merging extracted liquid, filtering, concentrating the filtrate, letting stand, filtering, separation to eliminate the impurity in the filtrate, concentration and drying. The water cress extract is used in treating hepatitis B and has the functions of resisting hepatitis virus, protecting liver and reducing alanine aminotransferase. It has definite curative effect and no toxic side effect.

The invention relates to the usage of a 2',5',6',7'-tetrahydroxyflavanonol and the derivative in treating pyohemia, belonging to flavanonol compound THF, which is extracted and separated from baikal skullcap root of Chinese herbal medicine. Through experiment showing, the THF has the advantages that: the THF has direct neutralization effect and reveals better dose effect relationship; the THF also reveals better dose effect relationship upon inhibition of RAW 264.7 cells activation LPS stimulation; the THF can lower LPS level in blood of LPS blood symptom model animal in vivo; the THF also has obvious protection effect upon bacterial sepsis model mouse caused by heat-inactivation escherichia coli with lethal dose; the THF has good application prospect under the condition of lacking effective means treating pyohemia in prior study.

The invention discloses a method for detecting the in-vitro activity of recombined human interleukin-12 by using a cell with an interleukin-12 receptor and application. The method comprises the following steps of (1) taking out the frozen cell with the interleukin-12 receptor from a liquid nitrogen tank, and performing recovery activation cultivation till the logarithm growth period is reached; (2) inducing the cell with the interleukin-12 receptor through a recombined human interleukin-12 standard substance under different concentrations to generate gamma interferon to obtain the concentration value ED50s of the recombined human interleukin-12 standard substances; (3) inducing the cell with the interleukin-12 receptor through a recombined human interleukin-12 protein to be detected under different concentrations to generate the gamma interferon to obtain a concentration value ED50r of the recombined human interleukin-12 protein to be detected; and (4) obtaining the in-vitro activity Pr of the recombined human interleukin-12 protein to be detected according to a formula Pr=Ps*ED50s / ED50r.

The invention relates to a traditional Chinese medicine preparation used for curing hemorrhoids and a preparation method thereof. The preparation is made from the active drug ingredients processed by dioscorea rhipogomoides, pod of pagodatree, cassia seed, oyster (calcined), ginseng, subprostrate sophora and honeysuckle and a pharmaceutically acceptable carrier according to a certain proportion by processing. The carrier is starch. Compared with the prior art, the traditional Chinese medicine preparation provided by the invention has obvious curative effects when being used for treating internal hemorrhoids of phase I and phase II.

The invention discloses a new application of sodium pyruvate, namely an application in preparation of a drug for reliving high-chlorine acidosis. Sodium pyruvate is taken as an active component to prepare the drug for reliving the high-chlorine acidosis, and the drug can be used for regulating the pH value of the blood to be normal and regulating the concentration of sodium ions, potassium ions and calcium ions in the blood so that the aim of reliving the high-chlorine acidosis is fulfilled. The drug disclosed by the invention has the following advantages: (1) the drug is obvious in curative effect and becomes effective rapidly; (2) the drug is free of toxic and side effects and high in safety; (3) sodium pyruvate is low in price; (4) the drug is convenient to carry and take.

The medicine for treating rheumatoid arthritis is prepared with mulberry twig, angelica, ledebouriella root and Sichuan achyranthes root in certain weight proportion, and through separate washing the materials, crushing, setting in extracting pot and adding water in 8-16 times, boiling for 0.8-1.2 hr, 200-mesh sieving to obtain filtrate, decompression concentrating to density of 1.05-1.08, 120-mesh sieving; repeating the above said process for twice; merging the filtrate of three times and spray drying to obtain the extracts of the materials; 60-mesh sieving, mixing the extracts and encapsulating to obtain medicine capsule. The medicine is suitable for preventing and treating rheumatoid arthritis.

The invention belongs to the field of natural medicines and discloses a compound medicine containing conjugated linoleic acid and luteolin and application thereof. The active ingredient of the compound medicine is composed of linoleic acid and luteolin at the mole ratio of (1:1) to (1:20). The medicine combining with linoleic acid and luteolin has a strong function of cooperatively treating lymphoma.

The invention discloses the application of nobiletin, in particular the application in the preparation of health products or medicines for preventing and / or treating oral cancer. The antiproliferative effects of nobiletin, naringenin and nobiletin on the human oral epidermoid cancer cell through the monomeric compounds nobiletin, naringenin and nobiletin in the citrus fruits of Rutaceae showed that nobietin, naringenin and nobiletin had the effect on human oral epidermoid cancer cells. The proliferation of cancer cells is obviously inhibited, and nobiletin has the most obvious effect of inhibiting the proliferation of oral cancer cells. It can be used to prepare health care products and medicines that can prevent and treat oral cancer, and is suitable for large-scale promotion.

The invention relates to fatty acid compounds and provides the fatty acid compounds and a preparation method and application thereof. The preparation method comprises the following steps of: boiling and refluxing angelica dahurica by using ethanol, filtering, and collecting filtrate to obtain angelica dahurica extracting solution; extracting the obtained angelica dahurica extracting solution, andperforming vacuum drying on solution obtained through extraction to obtain a crude angelica dahurica extract; and performing silica gel column chromatography on the crude angelica dahurica extract, and performing reversed phase silica gel column chromatography to obtain a compound 1, a compound 2 and a compound 3 respectively. The fatty acid compounds can be prepared into specific inhibitors for inhibiting protein tyrosine phosphatase-Src homology domain-containing protein tyrosine phosphatase 2 (PTP-SHP2), and achieve the effects of resisting cancer as well as preventing and treating PTP-SHP2-related human diseases by inhibiting PTP. The fatty acid compounds are widely applied to the preparation of medicines for preventing and treating cancer.

The invention provides a traditional Chinese medicine extract composition for treating cervical cancer. The traditional Chinese medicine extract composition comprises fructus forsythia and radix glycyrrhizae. The traditional Chinese medicine extract composition is a natural pharmaceutical composition creatively prepared by fructus forsythia ethanol extract and radix glycyrrhizae acetone extract according to the proportion of (1-4):1, and the optimal proportion obtained after repeated experiments is 2:1. Experiments prove that the traditional Chinese medicine extract composition has a proliferation inhibiting effect and an apoptosis-inducing effect on cervical cancer HeLa cells and has an extremely good antitumor effect, and the two traditional Chinese medicine extracts are combined to achieve a synergic effect, reduce drug use amount and lower toxic and side effects. The invention further provides application of the traditional Chinese medicine extract composition in the preparation of drugs for treating the cervical cancer. By the traditional Chinese medicine extract composition, an experimental foundation and scientific basis is provided for the application of traditional Chinese medicine compatibility to clinical antitumor drug researches.

The invention provides a method for rapidly detecting and diagnosing biological activity of in-vitro trace follicle stimulating hormone, which relates to the technical field of biomedicine and comprises the following steps: concentrating FSH (follicle stimulating hormone) from a small amount of urine sample; further removing LH from the concentrated FSH sample to obtain purified FSH; the method comprises the following steps: establishing an SNAP-FSHR HEK 293 stable expression cell strain; stimulating FSHR internalization by using the sample FSH; stimulating the FSHR by using the standardized FSH and causing in vivo retrogression of the receptor; acquiring an FSHR internalized image by utilizing microphotography; and calculating the number of internalized receptor particles by using Image J high-content image analysis software, and comparing the biological activity of the sample to be detected and the biological activity of the standard substance. The invention establishes and verifies a method for rapidly determining the biological activity of trace FSH in vitro by further analyzing high-content images by utilizing the reaction of FSHR stably expressed by a bioengineering cell strain to FSH. The FSH with LH removed can be rapidly recovered from human urine, and the recovery rate is about 90%. The internalization of the FSHR expressed in the HEK293 cell has a very good dose response relationship to trace FSH. The linear range is 0.01-50 ng / mL, and the correlation coefficient R2 is greater than or equal to 0.99.

The medicine for treating rheumatoid arthritis is prepared with mulberry twig, angelica, ledebouriella root and Sichuan achyranthes root in certain weight proportion, and through separate washing the materials, crushing, setting in extracting pot and adding water in 8-16 times, boiling for 0.8-1.2 hr, 200-mesh sieving to obtain filtrate, decompression concentrating to density of 1.05-1.08, 120-mesh sieving; repeating the above said process for twice; merging the filtrate of three times and spray drying to obtain the extracts of the materials; 60-mesh sieving, mixing the extracts and encapsulating to obtain medicine capsule. The medicine is suitable for preventing and treating rheumatoid arthritis.

The invention relates to fatty acid compounds, and provides an application of a fatty acid compound in preparation of medicines for preventing and treating the liver cancer. Angelica is boiled by ethanol, flows back and then is filtered, and a filtrate is collected, so that an angelica extract is obtained; the obtained angelica extract is extracted, an obtained solution after the extraction is subjected to vacuum drying, and a crude angelica extract is obtained; and the crude angelica extract is subjected to column chromatography on silica gel and column chromatography on reverse phase silica gel, so that a compound 1, a compound 2 and a compound 3 are obtained respectively. The fatty acid compound can be prepared into a specific inhibitor of PTP-SHP2; through inhibiting PTP (protein tyrosine phosphatase), the anticancer function and the function of preventing and treating human diseases relevant to PTP-SHP are played; and the fatty acid compound can be widely applied to preparation of medicines for preventing and treating cancers.

The invention belongs to the technical field of medicament and specifically relates to use of a deuteroporphyrin derivative. The invention provides use of deuteroporphyrin derivative in preparing medicament for treating squamous carcinoma or adenocarcinoma. The treatment with the deuteroporphyrin derivative or composition and medicament thereof to squamous carcinoma or adenocarcinoma has obvious growth-inhibiting effect and good dose effect relationship. A group with high dosage has strong and stable effect and stronger inhibiting effect than the positive control medicament, hemoporphyrin. The deuteroporphyrin derivative has no growth-inhibiting effect on tumour cells under a condition of non-laser radiation, no damaging effect on normal histiocytes with light or without light and little side effect.

The invention belongs to the field of natural drugs and discloses an adenosine and andrographolide compound drug composition and application thereof. An active ingredient of the compound drug composition consists of adenosine and andrographolide with a molar ratio of 1:1-1:20. The combination of the two drugs has a strong application to the synergistic treatment of lymphoma.

The invention discloses a new application of protopanoxadiol, and particularly relates to a new application of the protopanoxadiol serving as an active ingredient or a unique active ingredient in preparation of medicines for treating cancer fatigue. Animal experiment results show that the protopanoxadiol not only can obviously improve evaluation indexes of general conditions such as weight loss, mental state and diet condition of tumor-bearing chemotherapy mice in a cancer fatigue model, but also has an obvious treatment effect on physiological fatigue and psychological fatigue indexes relatedto cancer fatigue of the tumor-bearing chemotherapy mice. The technical scheme provided by the invention shows that the protopanoxadiol can improve the tumor microenvironment and the hematopoietic function of CRF mice. Acute toxicity and long-term toxicity tests also show that the protopanoxadiol is high in clinical medication safety, can be used for preparing drugs for treating cancer fatigue, and has important medicinal value.

The present invention relates to a method for setting the positive control of the external micronucleus test of the water extraction liquid of the cigarette filter. The method is as follows: 1) selecting the same number of cigarette filters meeting the conditions for preparing the positive control and the cigarette filter of the sample group to be tested; 2) The preparation concentration is 200-400? μg / ml cyclophosphamide aqueous solution; 3) Spray cyclophosphamide aqueous solution evenly on the cigarette filter tip used to prepare the positive control group, and wait for it to dry naturally for later use; 4) Use the same method for the positive control group and the test The sample group was extracted from the cigarette filter; 5) The extract was used as a solvent, prepared with powdered cell culture medium, and the prepared solution was filtered and sterilized; 6) The in vitro micronucleus test was carried out on the extract treated in step 5) . The positive control set by this method can verify whether the extraction process of the cigarette filter and the subsequent in vitro micronucleus test on the water extract of the cigarette filter are reliable.

The invention discloses a fusion protein of a nanobody and Pseudomonas aeruginosa exotoxin. The fusion protein is formed by cascading the nanobody with targeting function or its variable region fragment and the Pseudomonas aeruginosa exotoxin. The invention also provides the application of the fusion protein in the preparation of drugs for treating tumors. The fusion protein has good specificity and killing effect on tumor antigens, has protective effect on normal tissue cells, and has good clinical application prospect.

The invention discloses fusion protein of a nano antibody and pseudomonas aeruginosa exotoxin and application. The fusion protein is formed through connecting the nano antibody with a targeting effector a variable region fragment thereof and the pseudomonas aeruginosa exotoxin in series. The invention further provides application of the fusion protein to preparation of a medicine for treating tumors. The fusion protein has good specificity and a killing effect on tumor antigens, has a protection effect on normal tissue cells and has a good clinical application prospect.

The present invention belongs to the field of natural medicines and discloses a compound medicine composition with anti lymphoma activity containing genistein and emodin and use thereof. Active ingredients of the compound medicine composition comprise the genistein and the emodin in a molar ratio of 1:1 to 1:10. Through combination of the genistein and the emodin, the compound medicine composition has a strong synergistic treatment effect on lymphoma.