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Salvia minium phenolic acid A and process of preparing preparation and use

A technology of salvianolic acid and salvianolic acid, applied in the field of medicine, can solve the problems of high requirements for purified samples, high cost of reversed-phase silica gel, and low yield of salvianolic acid A, etc.

Inactive Publication Date: 2010-12-08
PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Chinese patent CN1513848A disclosed in 2002 the method of preparing salvianolic acid A by soaking in water, centrifugation, ultrafiltration, acid adjustment, organic solvent extraction and then reversed-phase column chromatography, using reversed-phase silica gel for purification, which has high requirements for the purified sample , the cost of reversed-phase silica gel is too high, and there are certain limitations in industrial mass production
Chinese patent CN1397276A, in 2003, disclosed the purification method of water or alcohol extraction, resin chromatography, and then silica gel column chromatography. Since the content of salvianolic acid A in medicinal materials is low, or even no, the yield of salvianolic acid A is low, and the actual It cannot fundamentally solve the industrial production problem of salvianolic acid A

Method used

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  • Salvia minium phenolic acid A and process of preparing preparation and use
  • Salvia minium phenolic acid A and process of preparing preparation and use
  • Salvia minium phenolic acid A and process of preparing preparation and use

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Experimental program
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Effect test

Embodiment 1

[0202] Embodiment one: the preparation method of salvianolic acid A, its processing step is:

[0203] 1. Take 100kg of Danshen crude powder, add 8 times the amount of 70% ethanol to reflux and extract 3 times, each time for 1.0 hour, and filter;

[0204] 2. Concentrate the combined filtrate to medicinal material: medicinal liquid (1:4), adjust the pH value of the concentrated solution to 6.0, place it in a reaction tank at 110°C, and have a gauge pressure of 0.05MPa, and react for 6 hours;

[0205] Gauge pressure 0.05MPa, reaction for 6 hours;

[0206] 3. Filter the reaction solution, pass the filtrate through the AB-8 type (manufactured by Nankai University Chemical Factory) macroporous adsorption resin column (the amount of medicinal material: resin is 1:2), wash with water until the sugar detection reaction is negative, and then use 10% ethanol Eluted to FeCl 3 If the reaction is negative, then elute with 30% ethanol, use HPLC to track and detect, collect the eluate conta...

Embodiment 2

[0210] Embodiment two: basically the same as embodiment one, the difference is that in step 3, the macroporous resin model is changed to HPD-100 (produced by Cangzhou Baoen Chemical Co., Ltd.); Amide chromatography column (medicine material: resin ratio: 1:2), wash with water until the sugar detection reaction is negative, then use 40% ethanol to elute to FeCl 3 If the reaction is negative, it is eluted with 80% ethanol, followed by high performance liquid phase detection, and the eluate containing salvianolic acid A is collected and concentrated under reduced pressure until there is no organic solvent; in step 5, the pH value is adjusted to 5.

[0211] It was determined that the content of salvianolic acid A was 97.97, the retention time of salvianolic acid A was 45.44 minutes, the retention time of impurity 1 was 32.14 minutes, the relative retention time of impurity 1 was 0.707, the content of impurity 1 was 0.57; the retention time of impurity 2 was 61.78 minutes, and the r...

Embodiment 3

[0212] Embodiment three: basically the same as embodiment one, the difference is that the macroporous resin in step 3 is changed to HPD-300 (produced by Cangzhou Baoen Chemical Co., Ltd.); step 4 is changed to filter the concentrated solution, and the polyamide layer on the filtrate Analyze the column (the amount of medicinal material: resin is 1:2), first wash with water until the sugar detection reaction is negative, then use 50% ethanol to elute until salvianolic acid A appears in the eluent, then elute with 95% ethanol, and use High-performance liquid phase tracking detection, collecting the eluate containing salvianolic acid A, and concentrating under reduced pressure until there is no organic solvent.

[0213] It was determined that the content of salvianolic acid A was 97.87, the retention time of salvianolic acid A was 45.594 minutes, the retention time of impurity 1 was 32.27 minutes, the relative retention time of impurity 1 was 0.708, the content of impurity 1 was 0....

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Abstract

This invention concerns the method of extracting salvianolic acid A from Chinese crude drug: danshen root, and the quality control methods and drug combinations, and the application of this drug. It can be used in the preparation of the prevention drugs for cardiovascular disease, liver damage, liver fibrosis, pulmonary fibrosis and other.

Description

technical field [0001] The present invention relates to a new salvianolic acid A extracted and separated from the traditional Chinese medicine Salvia miltiorrhiza for treating cardiovascular and cerebrovascular diseases, liver injury, liver fibrosis and pulmonary fibrosis, its preparation method, high-performance liquid phase determination method, and The pharmaceutical composition containing salvianolic acid A belongs to the technical field of medicine. technical background [0002] Danshen is one of the most commonly used medicinal materials for the treatment of cardiovascular and cerebrovascular diseases such as angina pectoris, hypertension, coronary heart disease and stroke. However, the crude extract extracted from Salvia miltiorrhiza or directly used in slices of Danshen is generally used. In the past two decades, salvianolic acid compounds, the main active components of Danshen, have been discovered one after another. The results of in vitro experiments show that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732A61K31/216A61K9/00A61K36/53A61P9/00A61P1/16A61K125/00
Inventor 李志刚顾群渠守峰栗艳彬
Owner PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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