34 results about "Flavanonol" patented technology

The invention discloses a method for increasing proanthocyanidin content in escherichia coli by cotransformation of brassica juncea gene BAN and DFR. According to the invention, an anthocyanidin reductase BAN gene and a 4-flavanonol reductase DFR gene are cloned from cDNA of brassica juncea purple-leaf mustard seed coat; a prokaryotic expression vector pET-BAN-DFR containing the BAN and DFR genesis constructed; and the BAN and the DFR genes are introduced into DH5alpha escherichia coli simultaneously. The transformed strain is cultured in an LB liquid medium at 25-37 DEG C at 150-250 rpm for6-12 hours, then cultured under shaking in an LB liquid medium containing black soybean seed coat extracts (0.5-1.0 g / L) at 25-37 DEG C at 150-250 rpm for 36-54 hours; the strain is collected by centrifugation; and the proanthocyanidin content in escherichia coli is determined by a DMACA-HCl method. The transgenic escherichia coli obtained in the invention has significantly increased proanthocyanidin content, and the maximum content can be 19.8 times higher than that of a non-transformed control strain.

The invention relates to application of lignin flavanonol in preparation of antiviral drugs, particularly, the invention relates to a chemical compound as formula (I), (+-)-2-[2,3-dihydro-2-(3,5-dimethoxy-4-hydroxy phenyl)-3-hydroxymethyl-1,4-benzodioxane-5]-2,3-dihydro-3,5,7-trihydroxy-4H-1-benzopyrone-4-ketone, or use of its medicinal salt used for preparing drugs for repressing virus infectioncaused by herpes simplex virus HSV-1 and / or drugs for curing oral ulcer. The chemical compound is prepared by chemical synthesis, pharmacological experiments shows that, the chemical compound has powerful inhibitory action to the herpes simplex virus HSV-1, and the half inhibitory concentration value IC[50] is 39.41 mg / mL.

The invention relates to a method for extracting 3,4',7-trihydroxy flavanonol and fustin with the antioxidant activity from varnish tree wood meal, and belongs to the field of development and application of plant extracts and chemistry of natural products. The varnish tree wood meal is used as a raw material, extraction, silica gel column chromatography and middle-lowerpressure chromatography are performed to obtain the 95% or above 3,4',7-trihydroxy flavanonol and fustin. The ingredients with the efficient antioxidant activity are obtained from a varnish tree, and the removal rate of DPPH, ABTS and hydroxyl radicals is higher than 95% when the concentration is 50 mg / L or above. The preparation process is rapid and simple, the extracted 3,4',7-trihydroxy flavanonol and fustin are good sources for developing a potential natural antioxidant, and the good commercial prospect is achieved.