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Compound medicine composition with anti lymphoma activity and use thereof

A composition and lymphoma technology, applied in the direction of drug combination, antineoplastic drugs, organic active ingredients, etc., to achieve the effects of reducing toxic and side effects, strong anti-proliferation activity, and good clinical drug development prospects

Inactive Publication Date: 2017-08-29
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combination of genistein and clinical drugs for the prevention and treatment of malignant tumor lymphoma has not been reported in the relevant literature almost no

Method used

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  • Compound medicine composition with anti lymphoma activity and use thereof
  • Compound medicine composition with anti lymphoma activity and use thereof
  • Compound medicine composition with anti lymphoma activity and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Screening of Daudi, Raji and BC-3 cells to optimize the compound pharmaceutical composition of genistein (GEN) and emodin (ED).

[0020] Take the cells in the logarithmic growth phase and inoculate 3×10 4 Cells / well were placed on a 96-well plate. After 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no drug group and drug-added group, and the drug-added group consisted of GEN and ED single drugs Group, GEN and ED combined drug groups with different molar ratios, set 4-6 duplicate wells for each group, culture for 24 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free culture medium containing 0.5 mg / ml for culture After 4 hours, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator to vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Results The inhibition rate of ...

Embodiment 2

[0026] Embodiment 2: GEN and ED combined administration effect analysis

[0027] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:

[0028] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentration, m is...

Embodiment 3

[0033] Embodiment 3: Genistein (GEN) and emodin (ED) compound pharmaceutical composition are made tablet according to following prescription:

[0034] The prescription is as follows:

[0035]

[0036] The preparation process is as follows:

[0037]The medicine and auxiliary materials are passed through 80 mesh sieves respectively, and emodin is fully mixed with 48 grams of microcrystalline cellulose and 12 grams of carboxymethyl starch sodium, 10% starch slurry is used as a soft material, granulated with 18 mesh sieves, and dried at 60 ° C. Particle 1 is obtained. Fully mix genistein with 24 grams of microcrystalline cellulose, 15 grams of starch and 8 grams of sodium carboxymethyl starch, make soft material with 10% starch slurry, granulate with 18 mesh sieves, and dry at 60° C. to obtain Granule 2. According to the principle of equal increase, fully mix granule 1 and granule 2, sieve the granules with 16 mesh, add magnesium stearate, mix well, and compress into tablets,...

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Abstract

The present invention belongs to the field of natural medicines and discloses a compound medicine composition with anti lymphoma activity containing genistein and emodin and use thereof. Active ingredients of the compound medicine composition comprise the genistein and the emodin in a molar ratio of 1:1 to 1:10. Through combination of the genistein and the emodin, the compound medicine composition has a strong synergistic treatment effect on lymphoma.

Description

technical field [0001] The invention belongs to the field of natural medicines, in particular to a compound pharmaceutical composition containing genistein and emodin with anti-lymphoma activity and its application. Background technique [0002] Cancer is a chronic disease that seriously endangers human health, and has become the second largest killer after cardiovascular disease. Therefore, it is of great significance to find safe, effective, and low-toxic antitumor drugs and study their mechanism of action. [0003] Genistein (GEN), one of the main active factors in soy isoflavones, is the most effective functional ingredient in soy isoflavone products and has a variety of physiological functions. Genistein is structurally similar to mammalian estrogen-estradiol, and has the active group of estrogen-diphenolic hydroxyl, so genistein has various physiological activities such as estrogen-like activity. GEN has weak estrogen activity, antioxidant activity, antihemolytic acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K31/122A61P35/00
CPCA61K31/122A61K31/352A61K2300/00
Inventor 刘连刘腾彭咏波邱桥春
Owner FOSHAN UNIVERSITY
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