The present invention provides an inverted QD-LED and a manufacturing method thereof. The manufacturing method of an inverted QD-LED according to the present invention adopts a hydrothermal synthesis process to form a monocrystalline TiO2 nanorod array film for serving as an electron transport layer, wherein a plurality of monocrystalline TiO2 nanorods contained in the monocrystalline TiO2 nanorod array film are arranged in an array so as not to readily get aggregated thereby overcoming the deficiencies of inhomogeneous film formation resulting from aggregation of TiO2 nanometer particles, lowered electron transport efficiency, and low light extraction efficiency, and thus ensuring high-efficiency electron transport rate of the electron transport layer, increasing scattering of light to heighten light extraction efficiency, and improve luminous efficiency and stability of a device. The inverted QD-LED according to the present invention involves a monocrystalline TiO2 nanorod array film to serve as an electron transport layer, and thus demonstrates increased luminous efficiency and stability.