47results about How to "Enhanced adhesion probability" patented technology

The invention provides a cell-biomaterial composite support frame. The composite support frame comprises a support frame base body and viscous cell suspension capable of slowly releasing cells, wherein the composite support frame forms the AC<n>B distribution formation from bottom to top, wherein the A is a support frame base body layer; the B is a viscous cell suspension layer; the C is a mixing layer formed by the viscous cell suspension through crossed distribution in support frame base body gaps; the n is a positive integer from 3 to 20; the viscous cell suspension comprises cells, cell carrying carriers and water; and the carriers are biocompatible viscous materials. The distribution density of the ells on the composite support frame is controllable; the adherence rate and the cell survival rate are high; and the problems of weak tissue regeneration promoting capability, weak proliferation and differentiation and slow growth of the cells on a three-dimensional composite support frame can be solved. The invention also provides a preparation method and application of the composite support frame.

The invention belongs to the technical field of composite stents, and discloses an RGD polypeptide modified chitosan / hydroxyapatite composite stent and a preparation method thereof. The method includes preparing a chitosan / nano hydroxyphosphorus composite porous material with double pores by using the in situ hybridization method; modifying RGD peptide on the surface of the material pores; conducting complex culture on the stent and MSCs and evaluating the short-term adherence rate of the stent on the MSCs cells and the effect on the adhesion microscopic morphology of the cells and the stent;manufacturing an artificial bone biological material. The chitosan / nano-hydroxyapatite composite porous material with the double pores is prepared by the in-situ hybridization method, and the RGD polypeptide is modified on the surface of the material pores by an electrostatic self-assembly method to obtain a three-dimensional porous stent. The composite culture of the stent and the MSCs cells is utilized to evaluate the short-term adhesion rate of the MSCs cells and the effect on the microscopic morphology of the cells and the stent to manufacture the artificial bone biological material to meet the clinical application.

The invention provides a low-order coal slurry flotation collecting agent compounded with acid aldehyde ether and a preparing method and application of the low-order coal slurry flotation collecting agent. The collecting agent comprises following components including, by mass percent, 60%-85% of C10-C16 hydrocarbon oil, 5%-15% of C6-C8 acid, 5%-15% of C5-C9 aldehyde and 5%-15% of C4-C10 ether. According to the provided low-order coal slurry flotation collecting agent compounded with the acid aldehyde ether, the collecting agent has the characteristics that components are simple, the collectingagent can serve as the low-order coal slurry flotation collecting agent to finish economical and efficient sorting of conventional flotation, reagent consumption is low, the flotation cost is low, and the sorting efficiency is high.

The invention provides a low-order coal slime flotation collector compounded with ester aldehyde ether and its preparation method and application. The collector has simple composition and can be used as a low-rank coal slime flotation collector to complete conventional The economical and efficient separation of flotation has the characteristics of small chemical consumption, low flotation cost and high separation efficiency. The low-rank coal slime flotation collector of compound ester aldehyde ether provided by the invention comprises each component of following mass percent: C 10 -C 16 Hydrocarbon oil 60‑85%; C 10 -C 18 Esters 5‑15%; C 5 -C 9 Aldehyde 5‑15%; C 4 -C 10 Ether 5‑15%.

The invention provides a low-rank coal slime flotation collector compounded with acid ketone ether and a preparation method thereof. In terms of mass percentage, the collector includes: C 10 -C 16 Hydrocarbon oil 60‑85%; C 6 -C 8 Acid 5‑15%; C 5 -C 10 Ketone 5‑15%; C 4 -C 10 Ether 5‑15%. The collector provided by the invention has simple components, can be obtained by compounding a small amount of reagents, and can be used in conventional low-rank coal slime flotation technology, with small reagent consumption, low flotation cost, and high separation efficiency. Significant economic benefits.

The invention discloses a method for inducing PC-12 cells to be differentiated into neurons. The method comprises the following steps: adding an induction medium and an NGF (nerve growth factor) into PC-12 cells, and performing induced differentiation to form neurons, wherein the induction medium is an opti-mem complete medium. According to the method disclosed by the invention, induction for inducing neurite length and form of cells to be similar to neurons can be obviously improved, the cell adhesion ability is excellent, induced cell proliferation is slow, and the PC-12 cell induction efficiency is greatly improved. The method disclosed by the invention can be widely applied to research models in neurobiology and neuropharmacology and can also be widely applied to neurobiological, neuropharmacological and toxicologic study.

The invention discloses a method for preparing a bacterial cellulose three-dimensional exhibition microporous bracket, and relates to the technical field of preparation and processing of bracket materials. The bacterial cellulose bracket is obtained by passivating, cutting and freeze-drying bacterial cellulose produced by fermentation of strains. By a computer tomography technology, a math model of a bacterial cellulose bracket three-dimensional structure is constructed; and the specific three-dimensional exhibition microporous bracket is designed by the math model. The math model for a required bacterial cellulose three-dimensional exhibition microporous structure is introduced into a carbon dioxide laser perforation machine and is processed under an environment of -5-10 DEG C; the processed bacterial cellulose bracket is cleaned by secondary distilled water; and then the bacterial cellulose three-dimensional exhibition microporous bracket is obtained by freeze-drying. According to the method, the technology is simple and convenient to operate; sizes of micropores and structures of three-dimensional exhibition holes of the bracket can be adjusted and controlled by methods, such as a method for controlling technical parameters; and the prepared three-dimensional exhibition microporous bracket can be applied to engineering field of construction of tissues, such as skins, bones, ribs and blood vessels.

The invention relates to the field of coal slurry pretreatment, in particular to an integrated refractory fine coal slurry adjustment equipment. The equipment includes a mixing box; the drive shaft is arranged vertically in the inner cavity of the mixing box, and the electric field activation layer, ultrasonic vibration layer and forced stirring layer are arranged in the inner cavity of the mixing box from top to bottom along the length direction of the drive shaft; The air box and the pressurized dissolved air box are arranged with an air inlet pipe for feeding high-pressure air, and the inlet end of the box body used to communicate with the outlet of the mixing box is arranged with a feed compartment for realizing the opening and closing of the inlet end. The feed partition is fastened with a tension spring and leans against the inlet end to close the inlet end; the outlet end of the box is connected to the flotation machine, and the outlet end is arranged to realize the opening and closing of the outlet. Partition, the discharge partition is tensioned by a compression spring and closes the outlet end. The invention can play a very good conditioning effect on the floating coal slime, and is especially suitable for the pulping and upgrading requirements of the difficult-to-float fine-grained coal, and has high working efficiency and quick and convenient pretreatment process.

The invention relates to the technical field of functional microorganism screening and application, and specifically provides a microecological composite additive containing Bacillus subtilis DN02 and its application in aquaculture. The Bacillus subtilis DN02 is screened from the intestinal tract of Litopenaeus vannamei, and the preservation number is CCTCC NO: M2020091, which can significantly inhibit pathogenic bacteria, improve the immunity and disease resistance of aquatic animals, produce protease and amylase to improve feed digestion and utilization, and promote The growth of aquatic animals can be widely used in the field of aquatic feed.