111 results about "Neural biology" patented technology

The invention discloses a three-dimensional flexible neural microelectrode and a manufacturing method thereof. The microelectrode uses a flexible polymer as a base material, and performs progressive electroplating through a circular pattern design of a metal seed layer to form smooth three-dimensional convex features. The electrode site structure can not only ensure good contact between the electrode site and the nerve cells, but also avoid damage to the nerve tissue caused by the sharp edges and corners of the raised electrode sites in the existing three-dimensional neural microelectrode. In addition, through the composite electroplating process in the electroplating process, that is, nano-scale dispersants are added to the electroplating solution to form a submicron-scale microporous structure on the surface of the electrode, increasing the surface area of ​​the electrode site, thereby enhancing the current output capability of the electrode and ensuring the nerve. Effective stimulation of microelectrodes under the constraints of biosafety conditions. The three-dimensional flexible neural microelectrode provided by the invention can be widely used in the fields of neurological disease treatment, neurological rehabilitation, neurobiological basic research and the like.

A digital test system for detecting the dynamic variation of two pupils is composed of a hardware system consisting of light isolating eye mask, miniature camera, focus distance and pupil distance regulator, image acquisition card and computer, and a software system comprising the image acquiring, extracting and storing module, image and video playback module, database and its management module, light stimulating program module, disease characteristics database module, expert database module, and expert judgment and management module.

A data acquisition system for use in conducting functional magnetic resonance imaging studies. fMRI studies can provide clues to the neurobiological basis of CNS disorders and reliable and quantifiable data relating to their symptoms. The data acquisition system includes a control station, a patient stimulus and response system and fMRI data acquisition software for controlling the operation of the system in response to operator input and acquiring fMRI data comprising MR images and associated behavioral response data. The stimulus and response system presents visual or auditory or other stimuli to the patient under direction of the control station while functional MRI scanning takes place. The MRI image data is collected from the MRI scanner and combined with the behavioral data generated as the patient responds to the stimuli and archived for later analysis and use. The system includes quality control measures for properly setting up the equipment and preparing and training the patient and for verifying data quality during the different steps in the process.

Characters of the invention are that polymer of possessing biocompatibility and flexibility are adopted for base material and fabricated structure of bulge electrode so as to guarantee that stimulation points and nerve are contacted fully in order to improve effect of recording stimulation of neuroelectricity and nervous signals. Preparing procedure includes steps: using anisotropic wet-process erosion to fabricate mould; forming silicon dioxide as sacrificial layer on surface of mouldof silicon oxide chip; through peeling off, glue injecting, and releasing sacrificial layer to obtain raised microelectrode of polymer. The disclosed method for preparing raised electrodes on polymer substrate is compatible to traditional technique of Micro-E1ectro-Mechanical System (MEMS). Features are: volume- producing standardized products. The invention is applicable to nerve rehabilitation, neurobiology etc. areas.

The invention discloses a making method of three-dimensional plant-typed micro-electrode array, which is characterized by the following: adopting metal yarn as electrode material directly; inserting metal yarn into fine flute of mould base; locating to break metal yarn through micro-fine pipe on the mould base by chemical erosion; forming point at breaking part; casting polydimethylsiloxane on the mould base; utilizing polymerized PDMS to form chassis support part to chuck metal yarn; stripping PDMS with metal yarn out of mould base; bombarding through oxygen ion to overlap and bond together; assembling three-dimensional micro-electrode array.

The present inventors have recognized that proper utilization of reconfigurable event driven hardware may achieve optimum power conservation in energy constrained environments including a low power general purpose primary processor and one or more electronic sensors. Aspects of neurobiology and neuroscience, for example, may be utilized to provide such reconfigurable event driven hardware, thereby achieving energy-efficient continuous sensing and signature reporting in conjunction with the one or more electronic sensors while the primary processor enters a low power consumption mode. Such hardware is event driven and operates with extremely low energy requirements.

Disclosed herein are compositions and methods for the treatment or prevention of neurological disorders using lynx compounds. The invention further discloses compositions and methods for the modulation of acetylcholine receptor activity.

The invention belongs to the field of cytobiology and neurobiology, and provides an establishment method and application of a clinical-grade neural stem cell line. The method under GMP (Good Manufacturing Practice) control comprises two steps, namely, primary culturing of neural stem cells, and subculturing of neural stem cells. The identification result shows that the neural stem cell line established by the method can express a plurality of stem cell markers, can be differentiated into nerve cells, astrocyte and oligodendrocyte, has high stability, and is suitable for long-term culturing; a large amount of sufficient clinical-grade neural stem cells can be obtained by the method. The method can be used for establishing the neural stem cell line based on a plurality of neural stem cells and even single neural stem cell, and thus the error in study can be reduced as far as possible. By adopting the neural stem cell line established by the method and the cells obtained by differentiating the neural stem cell line, the problems of the safety and effectiveness risk in cell therapy of nervous system disease can be solved effectively, and safe and effective treatment on such diseases becomes possible.

The invention relates to the field of cytobiology and neurobiology, in particular to an induction medium for inducing neural stem / progenitor cells to be differentiated into oligodendrocyte precursor cells and an induction method and application thereof. The invention adopts the technical scheme that after subjected to preprocessing culture for a certain time by a preprocessing medium, the human neural stem / progenitor cells are rapidly differentiated into the oligodendrocyte precursor cells for expressing oligodendrocyte precursor cell markers such as O4, A2B5, NG2, SOX10, PDGFR (Platelet Derived Growth Factor Receptor) and the like at a high purity under the action of the induction medium; and the key ingredients of the induction medium are bFGF (Basic Fibroblast Growth Factor), PDGF-AA and NT-3 (neurotrophins-3). The method disclosed by the invention and the obtained oligodendrocyte precursor cells can be applied to preparation of a medicament for treating the nervous system injury disease and have wide prospect on the aspects of experiment research and clinical treatment.

An apparatus and method for purification and assay of neurites is useful for separation and analyses of extension organelles and / or protrusion of cells for purification, production, observation, and quantification of neurites in the neurobiology field. The present invention provides a pore-sized controlled porous filter membrane which outspace side surface is coated with a cell adhesion layer to form an adhesion surface, and combines the neuronal cells with the porous filter membrane in an aqueous environment under conditions in which outgrown neurites from cell bodies of the neuronal cells are attracted to and grow on the adhesion surface, wherein the outgrown neurites of the neuronal cells pass through pores provided in the porous filter membrane to the adhesion surface while each of the pores has a size smaller than the cell bodies of the neuronal cells so as to prevent the cell bodies of the neuronal cells passing through the pores and remaining on an opposing side surface of the porous filter membrane.

The invention relates to a method and a device for correlated micro-imaging of a frozen light microscope and a frozen electron microscope, in particular to a method and a device for correlated imaging of a frozen light microscope and a frozen transmission electron microscope, including an optical microscope frozen object stage device used for optics-electron correlated micro-imaging and a corresponding correlated imaging method. By adopting the method and the device, optical micro-imaging at extra-low temperature (below minus 170 degrees) can be achieved, and frozen electron micro-imaging of the same samples in the same areas can also be achieved. The method and the device can be widely used to correlate various types of inverted optical microscopes and most transmission electron microscopes at the market, can achieve correlated imaging fast, conveniently and accurately, prevent ice crystals from forming on surfaces of the samples to the maximum extent, avoid problems of grid deformation and carbon film fracture, and can be widely applied to related research of cytobiology, neurobiology, biological medicines, etc.

The invention provides a three-dimensional solid needle tip flexible micro-electrode array and a making method thereof. The micro-electrode array comprises a split insulating column array wrapped in an insulating flexible substrate, an electrode and an electricity conducting lead, wherein the head of an insulating column is in a shape of a needle tip and wrapped by the electrode, the needle tip electrode is exposed outside, the electricity conducting lead is paved along the insulating column and the insulating flexible substrate, one end of the electricity conducting lead is connected with the electrode, and the other end is exposed outside. The insulating column is of a solid structure, so that the electrode can be pierced into a stimulation part and effectively provide electric stimulation and record. In order to make the three-dimensional solid needle tip flexible micro-electrode array, silicon materials are treated by a silicon dicing-corrosion process for making the three-dimensional solid needle tip flexible micro-electrode array. The three-dimensional solid needle tip flexible micro-electrode array provided by the invention can be used for nerve electric stimulation and record and can be widely used in the fields of nerve disease treatment, nerve rehabilitation, neurobiology basic research and the like.

Preclinical and clinical studies have shown that the autonomic nervous system (parasympathetic and sympathetic nervous systems) show reliable changes across the sleep wake cycle. Most importantly, the parasympathetic nervous system shows increased activity during sleep consistent with its role in regulating rest. Further, patients with insomnia show reduced parasympathetic activity and / or increased sympathetic activity during sleep consistent with a neurobiological model of “hyperarousal”. Described herein are methods and apparatuses that activate the parasympathetic nervous system in response to a diving reflex; a sustained diving reflex may, surprisingly, lead to enhancing sleep. The apparatuses and methods described herein may specifically and / or selectively activate this reflex may play a therapeutic role in the modulation of sleep in insomnia patients.

Deep Brain Stimulation (DBS) is taking off and will be part of the main treatment for brain diseases such as movement disorders, epilepsy, psychiatric diseases and many others. There is a need for more sophisticated devices that can do more in one penetration, not just stimulate. Once there is a probe in the brain, it is used for multiple passive measurements, without harming the brain further. It provides better understanding the brain and real time closed loop improved treatment. An apparatus and method are disclosed, which allow simultaneous monitoring of multiple parameters inside the human brain, such as: pH, temperature, pressure, seizure activity (EEG), degree of metabolism, oxygen tension in the brain, degree of excitotoxicity and others. The ability to measure those parameters during treatment and stimulation procedures makes the difference between success and failure of the patient.

This invention relates to a quick test method for testing the dynamic changes of pupils of drug-dependents and its device, which transmits the image data of the pupil to a video signal collecting card on a computer in the mode of video signals to be converted to digital signals via video modulus for further image process to be compared with database to judge if the tested one takes drugs. The device is composed of glasses and a computer, among which, a plastic cover blade is set on the glasses, an infrared micro-camera head is fixed on the cover blade, infrared light sources composed of LED are set at both sides and three LED at the right of the blade, data collection and a software are put in the computer.

The present invention relates to a method for measuring human intelligence using a neurobiogical model. The invention provides a method which enables neurometric IQ to be measured by processing the MRI and fMRI images of subjects to determine cortical thicknesses and brain activation levels, determining structural IQ (sIQ) and functional IQ (fIQ) from the cortical thicknesses and the brain activation levels, and using the structural IQ (sIQ) and the functional IQ (fIQ) as predictors to measure the neurometric IQ of the subject. According to the present invention, the concept of a neurometric IQ (nIQ) model is established and can assist in easily assessing individual differences in general cognitive ability. These results suggest that general cognitive ability can be explained by two different neural bases or traits: facilitation of neural circuits and accumulation of crystallized knowledge.

Linear conotoxin sequence and its encoded polypeptide sequence S6.8, its production and use are disclosed. The process is carried out by constructing cDNA library and cloning O-super-family conotoxin. It can delay vertebrate cerebral neuron cell membrane sodium-ion channel inactivation and sodium-ion channel opening time. It can be used as probe for ion-channel type and sub-type discrimination or as pilot compound to make research of medicine.

The invention discloses a four-color cognitive movement training 'tian'-shaped maze for pigeons. The maze comprises a maze body which is provided with an upper opening and formed by a periphery retaining plate and a bottom plate. Four cubic light boxes arranged on the base plate at intervals segment the inner cavity of the maze body into passages shaped like a Chinese character 'tian'. A lamp group formed by a violet light emitting lamp, a blue light emitting lamp, a green light emitting lamp and a yellow light emitting lamp is disposed in each light box. Detecting devices and awarding devices are disposed in the transverse and longitudinal passages of the passages shaped like a Chinese character 'tian'. An L-shaped metal support with a wire groove is fixed on the peripheral retaining plate. The front end of the transverse part of the L-shaped metal support is located right above the maze body, and a camera device and a neural signal detecting device are fixed at the front end of the transverse part. The maze has the advantages that a reliable automatic experiment device is provided for neurobiology research and neural signal detection of behavior functions such as special cognition, working memory and cognitive movement of pigeons.

The present invention relates to a preparation method and application of conotoxin gene Lt7.2 of Conus littertus Linnaeus of South China sea and polypeptide Lt7b encoded thereby. Through construction of a cDNA library, a conotoxin gene Lt7.2 is cloned by the invention from poison canal tissue of the Conus littertus Linnaeus of South China sea. The invention provides a preparation method of polypeptide. The conotoxin gene Lt7.2 and a vector pTRX are connected to get the recombinant expression vector pTRX-Lt7.2; the recombinant expression vector pTRX-Lt7.2 is transformed into host bacteria and expresses in the host bacteria stably and efficiently; and expression product recombinant fusion protein is separated and purified to obtain an objective protein polypeptide Lt7b. The polypeptide Lt7b provided by the invention has effects of neurotransmitter blocking transport and central analgesia and can be used for preparing tool medicine for the neurobiological research and analgesic drugs.

The present invention relates to a new-type drug-stopping medicine. Its raw material composition includes (by wt%) 0.01%-30% of amantadine hydrochloride or trihexyphenidyl hydrochloride or benserazide hydrochloride or memantine bydrochloride, 99.009%-65% of gamma-aminobutyric acid A receptor agonist raw material medicine or / and gamma-aminobutyric acid B receptor agonist raw material medicine, and the rest is auxiliary material. Said drug-stopping medicine can be made into various conventional dosage forms of tablet, capsule, granules, power, dripping pills and others.

The invention relates to a preparation method and an application of conotoxin striatus gene S21.1 in the South China Sea and the coded polypeptide S21a of the conotoxin striatus gene S21.1. According to the invention, a cDNA (complementary deoxyribonucleic acid) library is constructed, and the conotoxin striatus gene S21.1 is obtained by cloning in a tube of conus striatus in the South China Sea.The invention provides a preparation method of the polypeptide, which comprises the following steps: connecting the conotoxin gene S21.1 and a vector pTRX to obtain a recombinant expression vector pTRX-S21.1; transforming the recombinant expression vector pTRX-S21.1 into host bacteria and culturing and expressing in the host bacteria; and separating and purifying the recombinant fusion protein ofthe expression product to obtain the target protein, namely the polypeptide S21a. The polypeptide S21a provided by the invention has the functions of blocking neurotransmitter transfer and easing pains, and can be used for preparing tool medicines and analgesic medicines in neurobiology research.

The invention provides a Conus lividus neurotoxin gene 1v1.3 and a polypeptide 1v1c coded thereby and use of the polypeptide 1v1c in the preparation of tool medicines for neurobiological study and analgesic medicines. In the invention, a process of constructing a cDNA library is adopted to clone the conotoxin gene 1v1.3 in the toxin canal of Conus lividus, and through analysis on a base sequence with a tool software SEQTOOL, the mature peptide amino acid sequence of the polypeptide 1v1c coded by the Conus lividus neurotoxin gene 1v1.3 is determined to be represented by a sequence 400(2) in a sequence table. The polypeptide 1v1c provided by the invention can block the transmission of a transmitter of neuromuscular junction, and according to a mouse hot-plate method, the polypeptide 1v1c has pain relieving effect and can be used for preparing the tool medicines for neurobiological study and analgesic medicines.

The invention relates to a behavior test platform for detecting animal cooperation behaviors. The test platform which is a behavior detector for evaluating the cooperation ability of different animalscomprises a central control module, a photoelectric detecting module, a cooperation reward module and a shell device for fixing the modules above. The test platform has the advantages that the advanced cooperation behaviors of the animals can be standardized and quantified, the cooperation behaviors of to-be-tested animals can be detected in a certain period of time, the dynamic motion images ofthe to-be-tested animals can be collected through a camera, and the captured video signal is connected with a computer through an image collecting card and displayed and recorded in a computer window;simple and convenient equipment is provided for the researches of the animal cooperation behaviors, and the researches of related neurobiology can also be benefited.

The invention is a kind synchronous recording system for experience animal behavior and electrophysiology signal, which belongs to nerve biology experience device. The recording system includes a video gathering card, a camera, an aD sampling board, an electrophysiology signal amplifier, micro electrode, a computer which stores the video and electrophysiology signal gathering procedure in the memory. The invention stores the video and electrophysiology data through programming correspondent procedure, it corresponds the electrophysiology signal and the animal behavior and maintain the synchronization in the playback process. The gathering of video and the electrophysiology signal are integrated together. It provides several kinds of video compressing methods; it has multi-channel, multi-frequency electrophysiology data sampling rate and video sampling rate of 1-30 frames / second; provides video finding mode step-by-step; it can output the synchronization of electrophysiology data and the animal behavior in a high accurate time range.

The present invention is quick identification method of the addiction and dependence of opium drug addict, and belongs to the field of medicine and biotechnology. Naloxone eye drop in 2 or 3 drops is dripped into the eye of drug addict, and after 10-40 min, the pupil diameter is measured and compared with that before naloxone is dripped. The addiction and dependence on opium drug is judged based on the diameter difference, and the difference over 5 pixels shows the existing addiction, and the difference of 5-10, 11-20 and over 21 shows the slight, middle and serious dependence separately. The said method is efficient, accurate and reliable.

The invention relates to a novel M-superfamily toxin gene Lt3.1 of Conus litteratus, the coding peptide sequence lt3a and the application of the peptide in preparing the tool drugs and the analgesic drugs for the Neurobiological Study. The gene is cloned from the Conus litteratus venom tube by means of constructing a cDNA library.

The invention provides insect specific polyamine micromolecular toxin (denoted as AVTX-623), wherein the relative molecular weight is 622.3971 and the molecular formula is C28H50N10O6. The AVTX-623 has the following properties: (1) the AVTX-623 can specifically act on an insect sodium ion channel, and the action dose of the AVTX-623 on the insect sodium channel is ten times lower than that of ArgTX-636; (2) the toxicity result on the insect living body indicates that the half effective anesthetic dosage of the AVTX-623 on the insect is 20 times lower than that of the ArgTX-636; (3) for vertebrate NMDA receptor activity, the action dosage of the AVTX-623 is slightly lower than that of the ArgTX-636; (4) the selectivity activity of the AVTX-623 on the insects is much higher than that of the ArgTX-636. The invention further discloses a purification method of the AVTX-623. Pure AVTX-623 can be completely purified from venom of araneus ventricosus through three steps of ultra-filtration, ion-exchange column chromatography and reversed phase high-performance liquid chromatography, the operation steps are simple, the loss rate is low, and the practicability is high. The invention further discloses application of the AVTX-623. The AVTX-623 can be applied to preparation of insecticide or / and a neurobiological reagent and medicines.