RGD polypeptide modified chitosan/hydroxyapatite composite stent and preparation method thereof

Abstract

The invention belongs to the technical field of composite stents, and discloses an RGD polypeptide modified chitosan / hydroxyapatite composite stent and a preparation method thereof. The method includes preparing a chitosan / nano hydroxyphosphorus composite porous material with double pores by using the in situ hybridization method; modifying RGD peptide on the surface of the material pores; conducting complex culture on the stent and MSCs and evaluating the short-term adherence rate of the stent on the MSCs cells and the effect on the adhesion microscopic morphology of the cells and the stent;manufacturing an artificial bone biological material. The chitosan / nano-hydroxyapatite composite porous material with the double pores is prepared by the in-situ hybridization method, and the RGD polypeptide is modified on the surface of the material pores by an electrostatic self-assembly method to obtain a three-dimensional porous stent. The composite culture of the stent and the MSCs cells is utilized to evaluate the short-term adhesion rate of the MSCs cells and the effect on the microscopic morphology of the cells and the stent to manufacture the artificial bone biological material to meet the clinical application.

Description

technical field [0001] The invention belongs to the technical field of composite scaffolds, in particular to an RGD polypeptide modified chitosan / hydroxyapatite composite scaffold and a preparation method thereof. Background technique [0002] At present, the existing technology commonly used in the industry is as follows : Bone defect is a common clinical problem in orthopedics. Every year, as many as 2.2 million people in the world need bone grafting due to trauma, infection, tumor and other reasons. In the United States, about 60% of patients who need bone grafting use autologous bone grafts every year. For bone grafting, 34% use allogeneic bone grafting, and only 7% of patients use other bone grafting materials. For nonunions and bone defects caused by various bone diseases such as trauma and bone tumors, autologous or allogeneic bone grafts are usually used for treatment. Although these methods are also very effective, they still often encounter insufficient material ...

AI Technical Summary

Problems solved by technology

[0006] In summary, the problem of prior art is that : At present, the adhesion rate of bone cells in various scaffolds is poor; the proliferation effect of bone cells after adhesion is not good; the growth rate of cells is slow

[0007] The difficulty and significance of solving the above technical problems : At present, most of the researches on bone tissue engineering scaffolds require a long period of culture of cells and scaffolds in vitro, which not only prolongs the treatment time, but also increases the treatment cost and increases the suffering of patients.

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