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Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket

A bacterial cellulose, three-dimensional technology, applied in medical science, prosthesis, etc., can solve the problems of poor controllability of porosity and pore distribution, random pore size change, and low degree of interpenetration of pores, so as to improve cell adhesion rate, The effect of good mechanical strength and good structural stability

Active Publication Date: 2014-11-19
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can obtain successful tissue engineering scaffolds, the obtained tissue engineering scaffolds lack mechanical strength, low degree of interpenetration of pores, poor controllability of porosity and pore distribution, and random changes in pore size, thereby affecting cell growth. Vascularization of tissues, transport of nutrients, and excretion of metabolites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Soak the bacterial cellulose obtained from the fermentation and culture of Acetobacter xylinum in an 8% by weight NaOH aqueous solution, heat it at 30°C for 6 hours, and then repeatedly rinse with double distilled water to neutrality. Remove bacterial protein and residual medium adhering to the cellulose membrane. Mechanical cutting process it into a cube of 2cm×2cm×2cm. At -40°C, freeze-drying to obtain a bacterial cellulose scaffold.

[0021] 2. Using computer tomography technology to obtain tomographic images of the bacterial cellulose scaffold, process each tomographic image, and construct a digital model of the three-dimensional structure of the bacterial cellulose scaffold through computer simulation. Taking the center of gravity of the digital model as the origin, the inoculation plane when the bacterial cellulose scaffold is inoculated with cells is projected downward as the XY plane to establish a three-dimensional coordinate system. Use the digital model to ...

Embodiment 2

[0024] The bacterial cellulose obtained by fermentation and culture of Sarcina spp. was immersed in 4% by weight NaOH aqueous solution, heated at a temperature of 60° C. for 5 hours, and then repeatedly washed with double distilled water to neutrality. Remove bacterial protein and residual medium adhering to the cellulose membrane. Mechanical cutting processes it into a cylinder with a diameter of 1 cm and a height of 2 cm. At -30°C, freeze-drying to obtain a bacterial cellulose scaffold.

[0025] Computer tomography technology is used to obtain tomographic images of the bacterial cellulose stent, and each tomographic image is processed, and a digital model of the three-dimensional structure of the bacterial cellulose stent is constructed by computer simulation. Taking the center of gravity of the digital model as the origin, the inoculation plane when the bacterial cellulose scaffold is inoculated with cells is projected downward as the XY plane to establish a three-dimensional...

Embodiment 3

[0028] The bacterial cellulose obtained by fermentation and culture of Alcaligenes and Azotobacter is soaked in 1-8% by weight of NaOH aqueous solution, heated at 100° C. for 3 hours, and then repeatedly washed with double distilled water to neutrality. Remove bacterial protein and residual medium adhering to the cellulose membrane. Mechanical cutting is processed into a film-like material of 5cm×5cm×1cm. At -10°C, freeze-drying to obtain a bacterial cellulose scaffold.

[0029] Computer tomography technology is used to obtain tomographic images of the bacterial cellulose stent, and each tomographic image is processed, and a digital model of the three-dimensional structure of the bacterial cellulose stent is constructed by computer simulation. Taking the center of gravity of the digital model as the origin, the inoculation plane when the bacterial cellulose scaffold is inoculated with cells is projected downward as the XY plane to establish a three-dimensional coordinate system....

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Abstract

The invention discloses a method for preparing a bacterial cellulose three-dimensional exhibition microporous bracket, and relates to the technical field of preparation and processing of bracket materials. The bacterial cellulose bracket is obtained by passivating, cutting and freeze-drying bacterial cellulose produced by fermentation of strains. By a computer tomography technology, a math model of a bacterial cellulose bracket three-dimensional structure is constructed; and the specific three-dimensional exhibition microporous bracket is designed by the math model. The math model for a required bacterial cellulose three-dimensional exhibition microporous structure is introduced into a carbon dioxide laser perforation machine and is processed under an environment of -5-10 DEG C; the processed bacterial cellulose bracket is cleaned by secondary distilled water; and then the bacterial cellulose three-dimensional exhibition microporous bracket is obtained by freeze-drying. According to the method, the technology is simple and convenient to operate; sizes of micropores and structures of three-dimensional exhibition holes of the bracket can be adjusted and controlled by methods, such as a method for controlling technical parameters; and the prepared three-dimensional exhibition microporous bracket can be applied to engineering field of construction of tissues, such as skins, bones, ribs and blood vessels.

Description

Technical field [0001] The invention relates to the technical field of preparation and processing of biological scaffold materials. In particular, it relates to a preparation method of a bacterial cellulose three-dimensional display microporous scaffold. Background technique [0002] Tissue engineering scaffold materials are one of the three major elements of tissue engineering. Since human tissues have a specific macroscopic appearance, the scaffold materials required for tissue engineering repair also have corresponding macroscopic appearances. And because a large number of studies have shown that the microscopic (nano-scale, micro-scale) structure of scaffold materials used for tissue engineering repair can affect cell growth and protein expression. Therefore, in the preparation process of tissue engineering scaffold materials, it is necessary to control the microstructure while controlling the macroscopic morphology. The ideal tissue engineering scaffold material should hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/56
Inventor 钟春燕其他发明人请求不公开姓名
Owner 钟春燕
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