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Method for preparing bacterial cellulose three-dimensional exhibition microporous bracket

A bacterial cellulose, three-dimensional technology, applied in medical science, prosthesis, etc., can solve the problems of poor controllability of porosity and pore distribution, random pore size change, and low degree of interpenetration of pores, so as to improve cell adhesion rate, The effect of good mechanical strength and good structural stability

Active Publication Date: 2013-09-18
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can obtain successful tissue engineering scaffolds, the obtained tissue engineering scaffolds lack mechanical strength, low degree of interpenetration of pores, poor controllability of porosity and pore distribution, and random changes in pore size, thereby affecting cell growth. Vascularization of tissues, transport of nutrients, and excretion of metabolites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Soak the bacterial cellulose obtained from the fermentation culture of Acetobacter xylinum in 8% by weight NaOH aqueous solution, heat at 30°C for 6 hours, and then rinse repeatedly with twice distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane. Mechanically cut it into a cube of 2cm×2cm×2cm. Freeze-dry at -40°C to obtain bacterial cellulose scaffolds.

[0021] 2. Using computerized tomography technology to obtain the tomographic images of the bacterial cellulose scaffold, process each tomographic image, and construct a digital model of the three-dimensional structure of the bacterial cellulose scaffold through computer simulation. Taking the center of gravity of the digital model as the origin, the inoculated surface of the bacterial cellulose scaffold is projected downward to the XY plane to establish a three-dimensional coordinate system when the cells are inoculated. Using digital models to desi...

Embodiment 2

[0024] The bacterial cellulose obtained from the fermentation culture of Sarcina was soaked in 4% NaOH aqueous solution by weight, heated at 60°C for 5 hours, and then washed repeatedly with twice distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane. Mechanical cutting processed it into a cylinder with a diameter of 1 cm and a height of 2 cm. Freeze-dry at -30°C to obtain bacterial cellulose scaffolds.

[0025] The tomographic images of the bacterial cellulose scaffolds were obtained by computerized tomography technology, each tomographic image was processed, and a digital model of the three-dimensional structure of the bacterial cellulose scaffolds was constructed by computer simulation. Taking the center of gravity of the digital model as the origin, the inoculated surface of the bacterial cellulose scaffold is projected downward to the XY plane to establish a three-dimensional coordinate system when the cell...

Embodiment 3

[0028]Soak the bacterial cellulose obtained from the fermentation culture of Alcaligenes and Azotobacter in 1-8% NaOH aqueous solution by weight, heat at 100°C for 3 hours, and then rinse repeatedly with double distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane. It is machined into a film-like material of 5cm×5cm×1cm by mechanical cutting. Under the condition of -10°C, the bacteria cellulose scaffold was obtained by freeze-drying.

[0029] The tomographic images of the bacterial cellulose scaffolds were obtained by computerized tomography technology, each tomographic image was processed, and a digital model of the three-dimensional structure of the bacterial cellulose scaffolds was constructed by computer simulation. Taking the center of gravity of the digital model as the origin, the inoculated surface of the bacterial cellulose scaffold is projected downward to the XY plane to establish a three-dimensional ...

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Abstract

The invention discloses a method for preparing a bacterial cellulose three-dimensional exhibition microporous bracket, and relates to the technical field of preparation and processing of bracket materials. The bacterial cellulose bracket is obtained by passivating, cutting and freeze-drying bacterial cellulose produced by fermentation of strains. By a computer tomography technology, a math model of a bacterial cellulose bracket three-dimensional structure is constructed; and the specific three-dimensional exhibition microporous bracket is designed by the math model. The math model for a required bacterial cellulose three-dimensional exhibition microporous structure is introduced into a carbon dioxide laser perforation machine and is processed under an environment of -5-10 DEG C; the processed bacterial cellulose bracket is cleaned by secondary distilled water; and then the bacterial cellulose three-dimensional exhibition microporous bracket is obtained by freeze-drying. According to the method, the technology is simple and convenient to operate; sizes of micropores and structures of three-dimensional exhibition holes of the bracket can be adjusted and controlled by methods, such as a method for controlling technical parameters; and the prepared three-dimensional exhibition microporous bracket can be applied to engineering field of construction of tissues, such as skins, bones, ribs and blood vessels.

Description

technical field [0001] The invention relates to the technical field of preparation and processing of biological scaffold materials. In particular, it relates to a preparation method of bacterial cellulose three-dimensional display microporous support. Background technique [0002] Scaffold materials for tissue engineering are one of the three major elements of tissue engineering. Since human tissues have specific macroscopic shapes, the scaffold materials used for tissue engineering repair also have corresponding macroscopic shapes. And because a large number of studies have shown that the microscopic (nanoscale, microscale) structure of scaffold materials used for tissue engineering repair can affect cell growth and protein expression. Therefore, in the preparation process of tissue engineering scaffold materials, it is necessary to control the microstructure while controlling the macroscopic morphology. Ideal tissue engineering scaffold materials should have good tissue ...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/56
Inventor 钟春燕其他发明人请求不公开姓名
Owner 钟春燕
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