118 results about "Virgibacillus species" patented technology

The invention provides a bacillus subtilis for effectively degrading grease. The bacillus subtilis 6-loil is preserved at Guangdong Province Microbiological Culture Collection Center on December 22, 2016. The preservation number is GDMCC No. 60134. The bacillus subtilis 6-loil provided by the invention can utilize the grease as a growth carbon source, can normally grow under an environment of higher grease content and has a capacity of efficiently degrading grease. The bacillus subtilis 6-loil provided by the invention can be compounded with bacillus coagulans, brewer yeast and carrier ingredients so as to be prepared into a kitchen garbage disposal fungicide. The prepared kitchen garbage disposal fungicide can adapt to the condition of high grease content of the domestic kitchen garbage, so that the condition of failure in aerobic composting caused by the reduction of microbial activity resulted from the microorganism wrapped by the grease in aerobic composting can be effectively avoided and the bacillus subtilis can meet the requirement of the domestic kitchen garbage aerobic composting. The bacillus subtilis 6-loil also can be applied to the fields of soil oil stain treatment, biosurfactant production, biological detergent preparation, and the like.

The invention relates to a production method of bacillus subtilis antimicrobial lipopeptide and application thereof in feeds, belonging to the fields of feed additives and the biotechnology. The production method of the bacillus subtilis antimicrobial lipopeptide comprises the following steps of carrying out amplification culturing on an acillus subtilis fmbj seed culture medium, inoculating by 2-5 percent at the temperature of 28-35 DEG C, deeply fermenting 5000L of fermentor liquid for 30-48h, then extracting, and carrying out spray drying after concentration or directly carrying out spray drying to produce an antibacterial peptide product. The antimicrobial lipopeptide is added into basic daily feeds for broilers by a certain titer, thereby enhancing the feed utilization ratio and having better effect on growing development and immunity strengthening of broilers. The bacillus subtilis is a safe microorganism, and an antibacterial product thereof has high safety. In addition, compared with flavomycin used in the traditional broiler feeds, the bacillus subtilis antimicrobial lipopeptide has better effect and has an important significance on replacing feed antibiotic addition and guaranteeing food safety.

The invention belongs to the field of hygiene and relates to a bacillus subtillus degrading bacterial colony sensing signal and use of bacillus subtillus degrading bacterial colony as an antiseptic, in particular to bacillus subtillus degrading gram-negative bacterial colony sensing signal and the formation of a bacterial biofilm of the bacillus subtillus degrading gram-negative bacterial colony. In the use of the degrading bacterial colony sensing signal, a proper concentration of the bacillus subtillus is 5*102 to 5*104cfu / ml. The bacillus subtillus is further used as antiseptic to inhibit the bacteria such as pseudomonas, agrobacterium, enterobacteriaceae and vibrionaceae. The antiseptic can be ointment for external use, liquid preparation for external use, oral tablet, oral liquid, disinfectant, clearing agents or water quality improving agent. The consistency of the bacillus subtillus serving as the antiseptic is 105 to 106 cfu / g. The use of the bacillus subtillus provided by the invention and the antiseptic inhibit the formation of biofilm of disease-causing bacteria and the growth of bacteria by using microbial quorum-sensing inhibition instead of preventing and sterilizing bacteria by compound which is adopted by most of the conventional antiseptic; therefore, the problem of drug resistance in the antimicrobial process is avoided.

The invention relates to bacillus subtilis AnPL-1 capable of generating a lipopeptid surfactant under an anaerobic condition, with a preservation number of CGMCC NO. 13461. The strain AnPL-1 is a facultative anaerobe; a temperature range for efficient production of lipopeptid under the anaerobic condition is 30-40 DEG C; a pH (potential of hydrogen) value range is 6.0-7.5; a tolerance range of NaCl is 0-5%. An emulsion coefficient of an anaerobic fermentation solution of the strain for crude oil is equal to or greater than 56%; the diameter of an oil drainage circle is equal to or greater than 15mm; the lipopeptid surfactant produced by the strain AnPL-1 in situ in a physical simulation rock core increases a crude oil recovery factor by 9.9%. The strain has important popularization and application values in the field of microbial oil recovery and environmental pollution remediation.

The invention relates to a Taishan bacillus subtilis for degrading AFB1 (Aflatoxius B1). The bacillus subtilis is preserved in the China General Microbiological Culture Collection Center (CGMCC) since September 13, 2013, with a preservation number of CGMCC No. 8186, and the 16srDNA is shown in SEQ.NO.1. Experiments prove that after a fermentation liquor of the bacillus subtilis works with foldy feed at a temperature of 37 DEG C for 72 hours, active substances produced by the fermentation liquor can degrade AFB1 in the moldy feed; the bacillus subtilis has the advantages of high efficiency, mild acting effect, high safety, no influence on the original quality, simplicity of operations, low cost and the like, and is suitable for scale application in feed.

The invention relates to the technical field of applied biology, and aims to provide a bacillus subtilis strain and application of the bacillus subtilis strain in the preparation of a deodorizing microorganism. The bacillus subtilis strain is stored in China General Microbiological Culture Collection Center (CGMCC) of China Microbiological Culture Conservation Committee, and the conservation number is CGMCCNo.13714. The adopted technical scheme includes the steps of inoculating the bacillus subtilis CGMCC.13714 to an activated culture medium to obtain an activated strain, and inoculating the activated strain to an expanded fermentation medium for culture expansion to obtain a bacterial solution; inoculating the bacterial solution to faeces, and keeping the temperature at 20-35 DEG C. The novel Bacillus subtilis strain can be applied to the biological deodorization of faeces, and the deodorization effect of single use is stable and slightly influenced by the temperature; effective deodorization can be conducted by an in-situ deodorizing method, the operation is simple and easy, no supporting equipment and technology are needed, and the investment scale is small.

The invention discloses composite antioxidation peptides and a preparation method thereof. The fermentation substrates of the composite antioxidation peptides comprise the following raw materials in parts by weight: zein, soybean hydrolyzed protein and malt protein. According to the composite antioxidation peptides and the preparation method thereof, a bacillus subtilis seed bacterium suspension is used as a zymogen, the mixed dispersion system of the zein, the soybean hydrolyzed protein and the malt protein is used as a fermentation substrate, liquid state fermentation is performed, and a bacillus subtilis seed fermentation method and an enzymatic hydrolysis substrate are combined, so that a large number of functional antioxidation protein peptides can be decomposed and modified in the fermentation process. The composite antioxidation peptides and the preparation method thereof disclosed by the invention have the advantages that the technological processes are simplified, the preparation cost is reduced, and the products are diversiform. The prepared composite antioxidation peptides also have the advantages of being prominent in functions, high in rapid dissolving property, notable in processing characteristics, and the like, are suitable for the development of functional protein peptides having the effects of reducing blood pressure, resisting weariness, neutralizing the effect of alcoholic drinks, resisting oxidation and the like. According to the composite antioxidation peptides and the preparation method thereof disclosed by the invention, alcohol soluble protein is modified by using a fermentation method, so that the research stage is a climax period, and the problem that the functions of the single antioxidation peptide are deficient is solved.

The invention discloses bacillus subtilis cultured by biochar and a preparation method and an application thereof. The preparation method takes biochar as an adsorption carrier, such as wood chips, animal bones, plant rhizomes and other biochar, takes any kind of bacillus subtilis strains as a starting strain, and successively comprises the following steps: (1) test tube expanding culture; (2) liquid shake flask culture; (3) seed tank expanding culture; (4) fermentation culture; and (5) drying. The bacterial density of the bacillus subtilis prepared by the method can reach 5*10<13>-1*10<15> cfu / mL. The biological adsorption and the liquid fermentation are in organic combination, the bacterial density of the liquid fermentation is improved, no any additional equipment is added, continuous culture and semi continuous culture are not used, and the high-density culture of the bacillus subtilis is achieved; and the cultured bacillus subtilis can be used as a live bacterial agent of a biofertilizer or used as a live bacterial agent of the biofertilizer with other strains.

The invention belongs to the technical field of livestock-raising, in particular relates to the filtration of the separated bacterial strain of bacillus subtilis and an application of the bacillus subtilis in the pig manure composting production and biofermentation bed. The invention also belongs to the technical fields of agricultural microbiological application and the composting production. A bacillus subtilis dcy-1 suitable for the pig manure composting production and the biofermentation bed is obtained through separation, filtration and authentication, and the bacterial strain is persevered in China Center for Type Culture Collection (CCTCC) with the preservation serial number: CCTCC NO: M 208122. The bacterial strain is made into microbial inoculums and successfully applied to the pig manure composting production and scale raising cabiofermentation bed. The invention is suitable for the livestock feces treatment of scale raising piggeries.

The invention relates to bacillus subtilis and a culture method and application thereof. The bacillus subtilis ASD05 is preserved in the General Microbiology Center of the China Committee for CultureCollection of Microorganisms on May 15, 2019, the preservation address is No.3, No.1 Courtyard, Beichen West Road, Chaoyang District, Beijing City, and the preservation number is CGMCC No.17807. The invention further relates to the culture method and application of the bacillus subtilis ASD05. It is found for the first time that the bacillus subtilis ASD05 has the effect of adhering microorganismsrich in straw degradation effect to the surface of straw, and meanwhile has the capacity of high yield of xylanase and pectinase, cellulase activity is low, and laccase activity is avoided.