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Bacillus subtilis dcy-1 and application thereof in biofermentation

A Bacillus subtilis, bio-fermentation technology, applied in the field of agricultural microorganisms and animal husbandry, can solve the problems that have not been seen in the screening of Bacillus subtilis

Inactive Publication Date: 2009-09-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there have been no reports on the screening of high temperature-resistant Bacillus subtilis and its application in pig manure composting and fermentation beds

Method used

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  • Bacillus subtilis dcy-1 and application thereof in biofermentation
  • Bacillus subtilis dcy-1 and application thereof in biofermentation
  • Bacillus subtilis dcy-1 and application thereof in biofermentation

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Embodiment 1

[0018] 1. Isolation and cultivation of microorganisms

[0019] 1.1 Sampling: Weigh 5g of pig manure compost samples (with a pore size of 1mm, that is, No. 18 sieve) and place them in a 50mL beaker, add glass beads, add 25mL of distilled water with a measuring cylinder, and place on a shaking table for 20 minutes to fully disperse the samples. And disperse the microbial cells and let it stand still. Then boil it with boiling water for about 15 minutes to kill other microorganisms and make the bacillus produce spores to obtain a diluted bacterial solution containing the bacillus.

[0020] 1.2 Preparation of diluted bacterial solution: use a sterile straw to absorb step (1) 10 -1 Transfer 1mL of the diluted bacterial solution into a test tube filled with 9mL of sterile water, blow and inhale 3 times, let the diluted bacterial solution fully mix, and serve as 10 -2 Dilute the bacterial solution; then use a sterile straw to draw 10 -2 Transfer 1mL of the diluent into a test tube...

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Abstract

The invention belongs to the technical field of livestock-raising, in particular relates to the filtration of the separated bacterial strain of bacillus subtilis and an application of the bacillus subtilis in the pig manure composting production and biofermentation bed. The invention also belongs to the technical fields of agricultural microbiological application and the composting production. A bacillus subtilis dcy-1 suitable for the pig manure composting production and the biofermentation bed is obtained through separation, filtration and authentication, and the bacterial strain is persevered in China Center for Type Culture Collection (CCTCC) with the preservation serial number: CCTCC NO: M 208122. The bacterial strain is made into microbial inoculums and successfully applied to the pig manure composting production and scale raising cabiofermentation bed. The invention is suitable for the livestock feces treatment of scale raising piggeries.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry, in particular to the screening of an isolated Bacillus subtilis strain and its application in pig manure composting production and fermentation beds. The method is applicable to the treatment of livestock and poultry manure in large-scale pig farms, and belongs to the technical field of agricultural microbe application. Background technique [0002] Due to the rapid development of large-scale and intensive breeding industry, the environmental pollution of livestock and poultry breeding has become a serious social problem, and has attracted great attention and attention from the society. As a product that maintains good environmental effects, composting has the characteristics of sustainable biological treatment and recycling of waste resources. It has been accepted by many countries and regions and has become one of the effective methods for dealing with organic solid waste. The essence...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00A01K1/00C12R1/125
CPCY02W30/40
Inventor 邓昌彦武亮方莉玉石太莉程燕郭玲
Owner HUAZHONG AGRI UNIV
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