The invention relates to the technical field of lubricating fluid, in particular to a medical vagina lubricating fluid and a preparation method thereof. A medical vagina lubricating fluid comprises the following components in percent by weight: 7-15% of glycerinum, 7-16% of almond oil, 3-6% of camellia oil, 1.5-2.3% of sodium carboxymethylcellulose, 2.7-3.2% of water-soluble chitosan, 0.3-0.7% of natamycin, 2-4.5% of vitamin E, 0.15-0.3% of ceramide, 0.4-0.7% of mint oil, and the balance of purified water. According to the invention, the vagina environment can be effectively improved, the lubricating and moisturizing effects can be achieved, the vagina mucosa nerve sensitivity is improved, and the lubricating fluid is sanitary and safe; the use effect is excellent.