35 results about "Strict anaerobe" patented technology

The invention relates to a process for producing clostridium butyricum by high-density fermentation under a negative pressure condition. The process comprises the following steps of inoculating a clostridium butyricum seed solution into a fermentation culture medium, carrying out anaerobic fermentation under the conditions that the temperature is 35-40 DEG C, the stirring rotating speed is 100-300r/min, the pH value is constant to 6.0-7.5 by using soluble alkali, and the vacuum degree is kept to 0.03-0.08 Mpa under negative pressure air exhaust, culturing for 10 hours, and ending the fermentation. Vacuum is maintained through negative-pressure air exhaust, and stirring is started, a strict anaerobic environment is created, and a fermentation medium and a fed-batch fermentation process are optimized based on amino acid characteristics of clostridium butyricum thalli and spores, so that accumulation of metabolic harmful substances is reduced to the maximum extent, proliferation of clostridium butyricum and spore transformation are promoted, and the number of spores in the obtained clostridium butyricum fermentation liquor reaches 7.9 billion CFU/mL or above. The process for producing the clostridium butyricum through fermentation has the advantages that inert gas does not need to be introduced, the fermentation period is short, post-treatment is simple, the production cost is greatly reduced on the whole, the spore number of clostridium butyricum clear liquid fermentation is greatly increased, and meanwhile the clostridium butyricum with high cost performance can be produced.