30 results about "Sprivivirus" patented technology

Herein disclosed are rapid real-time isothermal multiplex methods of detecting, identifying and quantifying bacterial, viral, and protozoan nucleic acids in a sample. These include contacting the sample with two or more sets of pathogen-specific reverse transcription loop-mediated isothermal amplification primers and novel oligofluorophores specific for the target bacterial, viral, and parasitic nucleic acids of interest such as human immunodeficiency virus, Ebola virus, Marburg virus, Yellow fever virus, hepatitis-B virus, Lassa fever virus, Plasmodium, hepatitis-C virus, hepatitis-E virus, dengue virus, Chikungunya virus, Japanese Encephalitis virus, Middle Eastern Respiratory Syndrome Corona virus, Mycobacterium, West Nile virus, Cytomegalovirus, Parvovirus, Leishmania, Trypanosoma, and Zika virus nucleic acids, under conditions sufficient to produce detectable real-time amplification signals in about 10 to 40 minutes. The amplification signals are produced by pathogen-specific fluorogenic labels included in one or more of the primers. Also, novel reaction and sample lysis buffers, primers, and kits for rapid multiplex detection, quantification, and identification of bacterial, viral, and protozoan nucleic acids by real-time isothermal amplification are herein disclosed.

The invention provides fusion protein 210 and applications thereof in virus replication optimization. The fusion protein 210 is a truncated part of P60 protein, preserves a function for promoting replication of a plurality of viruses of the P60, and does not generate toxicity on cells in an effective concentration range. The fusion protein 210 can increase the virus titer by two orders of magnitudes in a wide-spectrum range (0.0001-0.1 MOI), and therefore virus identification and detection can be facilitated, rhabdovirus VSV, flaviviridae HCV, parvoviridae EV71, and the like can be detected, and time for detection is shortened by 50% or less. The fusion protein 210 obtained by a prokaryotic expression system is high in expression efficiency and easy to purify, the one-step purification efficiency can be 85% or above, and the protein final concentration can be 3 mg / mL. Accordingly, through directly adding the fusion protein 210 into a virus culture medium, a plurality of virus isolate strains can be detected under low titer conditions, and operation is simple, convenient and rapid. The fusion protein 210 has important influences and significance in the fields of production, learning, researching, and the like of the virology.

The invention discloses the application of compound PS-341 in the preparation of Picornaviridae Enterovirus inhibitors. The compound PS-341 has a structure shown in formula (I): the Picornaviridae Enterovirus virus For EV D68 virus, CV A16 virus. Experiments have proved that the compound PS‑341 inhibits the replication function of enteroviruses EV D68 and CV A16, and provides an effective solution for the prevention and treatment of enteroviruses EV D68 and CV A16.

The invention belongs to the field of virus detection, and specifically discloses a LAMP primer for detecting tilapia parvovirus and application thereof, The inventors isolated a parvovirus from the infected tilapia adult fish, which was named tilapia parvovirus (Tilapia parvovirus) TiPV, and its deposit number was CCTCC NO: V201856. It is of pioneering significance for the research of tilapia parvovirus. The primer designed for the specific sequence of the virus can be used to detect tilapia parvovirus. The primer has good specificity and high sensitivity.

The invention discloses a Seneca Valley virus (SVV) / CH / ZZ / 2016 strain with the specific physical and chemical characteristics. The Seneca Valley virus (SVV) / CH / ZZ / 2016 strain is a small non-envelopedRNA virus, and belongs to the small RNA virus family. The virus is resistant to organic solvents, protease and acid, but sensitive to alkali and shows sensitivity to high temperature. The physical andchemical characteristics of the Seneca Valley virus(SVV) / CH / ZZ / 2016 strain are of great significance for the research on how to prevent and control SVV epidemic and SVV vaccine development.