The invention provides
fusion protein 210 and applications thereof in
virus replication optimization. The
fusion protein 210 is a truncated part of P60
protein, preserves a function for promoting replication of a plurality of viruses of the P60, and does not generate
toxicity on cells in an effective concentration range. The
fusion protein 210 can increase the
virus titer by two orders of magnitudes in a wide-spectrum range (0.0001-0.1 MOI), and therefore
virus identification and detection can be facilitated, rhabdovirus VSV,
flaviviridae HCV, parvoviridae EV71, and the like can be detected, and time for detection is shortened by 50% or less. The fusion
protein 210 obtained by a
prokaryotic expression system is high in expression efficiency and easy to purify, the one-step purification efficiency can be 85% or above, and the
protein final concentration can be 3 mg / mL. Accordingly, through directly adding the fusion protein 210 into a virus culture medium, a plurality of virus isolate strains can be detected under low
titer conditions, and operation is simple, convenient and rapid. The fusion protein 210 has important influences and significance in the fields of production, learning, researching, and the like of the
virology.