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Strains, application of strains, vaccine and preparation method of vaccine

A strain and vaccine technology, applied in the biological field, can solve the problem of low cross-protection rate, reduce mink stress response, have good market application prospects, and prevent mink hemorrhagic pneumonia

Inactive Publication Date: 2016-06-29
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The serotypes of the vaccine strains used in the above-mentioned mink hemorrhagic pneumonia mainly include 2 or 3 types, namely, G type, B type, or G type, C type, and I type. Epidemiological survey, from 2010 to 2015, new serotypes F, I and C appeared in the epidemic serotypes, and due to the low cross-protection rate among various epidemic serotypes, it was about 20%-30%

Method used

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  • Strains, application of strains, vaccine and preparation method of vaccine
  • Strains, application of strains, vaccine and preparation method of vaccine
  • Strains, application of strains, vaccine and preparation method of vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation of Mink Parvovirus Enteritis and Hemorrhagic Pneumonia Dual Inactivated Vaccine

[0057] 1. Standards for vaccine strains:

[0058] 1.1 Pseudomonas aeruginosa strain standard

[0059] 1.1.1 Morphological and biochemical characteristics The bacteria are Gram-negative bacilli. The biochemical characteristics should conform to the characteristics of the bacteria in bacterial taxonomy.

[0060] 1.1.2 Culture characteristics Inoculate on a PYG agar plate and culture at 36-37°C for 18-24 hours. Visual observation shows that the colonies are flat and moist, with irregular edges, and produce green or brown pigments that diffuse in the medium; inoculate with PYG liquid In the culture medium, culture at 36-37°C for 18-24 hours, the liquid will be evenly turbid and produce green or brown pigment.

[0061] 1.1.3 Serological characteristics Use Pseudomonas aeruginosa type serotype, DL15 strain should be G type, JL08 strain should be B type, WD01 strain shoul...

Embodiment 2

[0145] Embodiment 2 safety test

[0146] Table 2 Safety test inspection content

[0147]

[0148] 1.1 Safety test

[0149] Three batches of vaccines 201501, 201502, and 201503 were inoculated in a single dose, repeatedly inoculated with a single dose, and overdose inoculated minks of different ages for safety evaluation. A single dose was inoculated with young minks aged 60 to 70 days, 1ml / mink; single-dose repeated inoculation Young minks aged 60-70 days, with an interval of 15 days between two vaccinations, each inoculation of 1ml / mink; over-dose inoculation of young minks aged 60-70 days and adult minks aged 3-6 months, 3ml / mink, injected in 3 points . In each group, 5 minks were inoculated, and the inner muscles of the hind limbs were inoculated, and a non-immunized control group was established. Continuously observe for 15 days after inoculation, observe systemic clinical symptoms and local changes of inoculation, and record body temperature changes every morning for ...

Embodiment 3

[0155] Efficacy Evaluation of Embodiment 3MEV-PA Dual Inactivated Vaccine

[0156] 1. Efficacy test of MEV-PA dual inactivated vaccine

[0157] 1 immune group

[0158] 1.1 Mink

[0159] Take 120 3-month-old healthy susceptible minks (healthy susceptible mink standard: HI antibody titer in mink parvovirus serum ≤ 1:4, agglutination reaction of Pseudomonas aeruginosa G, B, C, I, F mink serum slides Negative) were randomly divided into 4 groups, 40 in each group, divided into MEV-PA group, MEV group, PA group and control group, respectively intramuscular injection of vaccine 1ml / rat, the control group was injected with the same volume of normal saline in the same way.

[0160] 1.2 Mice

[0161] Fifty mice with a body weight of 16-18 g were divided into 5 groups, 10 mice in each group, and 0.2 ml of the vaccine was injected subcutaneously.

[0162] 2 Evaluation of immune effect on mink hemorrhagic pneumonia

[0163] 2.1 Mink

[0164] 2.1.1 Virus attack protection

[0165] 2...

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Abstract

The invention relates to the field of biotechnology, in particular to strains, application of the strains, a vaccine and a preparation method of the vaccine, and provides a combined inactivated vaccine for mink parvoviral enteritis and hemorrhagic pneumonia and a preparation process of the combined inactivated vaccine. The (bacterium) virus strains of the vaccine include the mink enteritis parvovirus strain (MEVB strain) and the mink pseudomonas aeruginosa G-type DL15 strain, B-type JL08 strain, C-type WD01 strain, I-type WF05 strain and F-type DL03 strain which are popular in China. The parvoviral enteritis strain and the pseudomonas aeruginosa strains serving as antigens are cultured, inactivated with formaldehyde and then mixed in proportion, aluminium hydroxide colloid is added in proportion, and the vaccine is prepared. The immunity period of the vaccine is 6 months, the immunity protection rate is 80% or above, and the vaccine can be used for preventing mink parvoviral enteritis and mink hemorrhagic pneumonia at the same time, reduces the immunization workload of raising households and the stress response of minks and has good market application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to bacterial strains and applications thereof, as well as vaccines and preparation methods thereof. Background technique [0002] Fur animals mainly refer to animals whose products are fur raw materials. With the development of the economy, the improvement of people's living standards and the continuous growth of demand, China's fur industry has shown a strong development trend. my country's fur animal breeding industry is mainly concentrated in Shandong, Hebei, Liaoning, Heilongjiang and other places. The breeds mainly include mink, raccoon dog, fox, and rex rabbit. The annual output of mink skins is 6 million pieces and fox skins are 2 million pieces. Among them, fox skins account for the world. 35% of the total output, mink fur accounts for 12% of the world's total output. After years of development and accumulation, fur animal breeding has become a dominant industry, and my country...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/104A61K39/295A61K39/23A61P31/04A61P31/20C12R1/385
CPCA61K39/104A61K39/12A61K2039/521A61K2039/5252A61K2039/552A61K2039/55505A61K2039/70A61P1/00A61P11/00A61P31/04A61P31/20C12N2750/14334C12N1/205C12R2001/385
Inventor 闫喜军白雪鲁荣光胡博吴威柴秀丽赵建军张海玲张蕾刘昊
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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