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35 results about "Seminiferous tubule structure" patented technology
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Seminiferous tubule in cross-section (large tubular structure - center of image) with sperm (black, tiny, ovoid bodies furthest from the outer edge of the tubular structure).
Fixed-point gene editing method based on intratestis injection
InactiveCN105296537AVector-based foreign material introductionAnimal husbandryMatingGene Modification
The invention discloses a simple, convenient and efficient fixed-point gene editing method relating to the fields of transgenic animal preparation, gene functional study and biomedicines. A testis injection method is a sperm-mediated gene transferring method developed by utilizing the sperm capacity of actively combining, transferring and integrating an exogenous DNA. By using the method disclosed by the invention, an animal testis injection method is optimized, a carrier constructed by utilizing a clustered regularly interspaced short palindromic repeat system (CRISPR / Cas9) is integrated with a sperm chromosome through multi-point testis injection or seminiferous tubule microinjection, so that the aims of deleting, replacing and inserting genes are achieved. Then, a transgenic animal descendant is obtained through various approaches such as natural mating and artificial insemination. According to the method, the advantages of a CRISPR / Cas9 gene editing system are sufficiently utilized, and the existing transgenic animal system is combined, so that the complexity of in-vitro cell operation and requirement on expensive precise instruments / equipment are avoided while the gene modification animal obtaining efficiency is greatly increased.
Owner:SOUTHWEST UNIVERSITY
Novel growth hormone releasing hormone analog peptides and application thereof in preparing medicines for treating infertility
The invention discloses novel growth hormone releasing hormone analog peptides and an application thereof in preparing medicines for treating infertility. Experiments discover that 2D, 2E or 2F peptide has obviously high hypophysis GH releasing activity and hypophysis hormone releasing specificity. Tested by the conjugation reaction of an in-vitro GHRH dimer peptide and a hypophysis GHRH receptor, 2D, 2E and 2F dimer peptides have extremely high hypophysis receptor binding activity, wherein the 2F dimer peptide has the maximum binding activity. With the 2F peptide as the representative, infertility model treatment finds out that, compared with a normal saline group and a pure cyclophosphamide control group, in the 2F dimer peptide group, the spermatocytes and the spermatogonia in the seminiferous tubules are obviously increased, the seminiferous tubule cells are arranged in order and large in volume, the cavities of the seminiferous tubules are reduced and even disappear, and dose dependency is shown. All the facts indicate that the GHRH peptides with the 2F peptide as the representative have an obvious effect of stimulating the proliferation and the maturation of spermatogonia / oogonia, thereby promoting reproduction; and as a result, the novel growth hormone releasing hormone analog peptides can be applied to medicines for treating infertility.
Owner:深圳纳福生物医药有限公司
Method for separation and purification, long-term passage cultivation, cryopreservation and recovery of sheep spermatogonia stem cells
ActiveCN104004708AHigh puritySolve the problem of not finding antibodiesDead animal preservationArtificial cell constructsStem Cell IsolationSingle cell suspension
The invention relates to a method for separation and purification, long-term passage cultivation, cryopreservation and recovery of sheep spermatogonia stem cells. The method includes the steps that various solutions are prepared, 2-3 g of tissue blocks of the seminiferous tubule are taken out from the testis of a sheep of 3-4 mouths of age, single-cell suspension is prepared through enzymolysis and digestion of two steps, purified spermatogonia stem cells are separated according to the difference adherence method, the long-term passage cultivation of the sheep spermatogonia stem cells is achieved through an SSCM culture solution, and the sheep spermatogonia stem cells are cryopreserved and recovered after the long-term passage cultivation. By the technical scheme, the sheep spermatogonia stem cells with high purity can be obtained without using specific sheep spermatogonia stem cell antibodies, a long-term passage cultivation system of the sheep spermatogonia stem cells is built, and the method for preserving the sheep spermatogonia stem cells for a long term through a liquid nitrogen freezing method is successfully provided. The sheep spermatogonia stem cells achieving separation, purification and passage according to the method are cultivated at least for more than 40 generations, and the longest cultivation period reaches four years if the cryopreservation time is included.
Owner:INNER MONGOLIA UNIVERSITY
Separation and culture method and application of testicle mesenchymal stem cells
The invention discloses a separation and culture method and application of testicle mesenchymal stem cells. The method comprises the following steps: separating a testicle from a 7-day or 2-month mouse, removing the envelope and vessels to expose a seminiferous tubule, digesting the seminiferous tubule with collagenase IV, and filtering the digested cell suspension; and on the basis of the cell specific marker of CD51, separating with a flow cytometry to obtain single cells, and collecting the cells for expressing CD51, thereby separating the CD51 positive testicle mesenchymal stem cells. The invention also discloses testicle mesenchymal stem cells separated according to the method, self-renewal and proliferation cells with multi-directional differentiation potential obtained in a culture medium, and application of the cells in preparing compositions for treating diseases related to low testosterone level.
Owner:SUN YAT SEN UNIV
Intratesticular injection of chemical sterilant
A low effective dose of a biologically acceptable chemical sterilant is injected into the dorsal cranial portion of a scrotal testis of a male animal for the purpose effecting sterilization of the animal. The injection of the chemical sterilant into the dorsal cranial portion has an effect on the epithelium of the tubuli recti, rete testis and ductus efferentes in addition to stopping spermatogenesis in the seminiferous tubules.
Owner:ARK SCI
Method for directionally inducing and differentiating pig pluripotent stem cells into male germ cells and special culture medium
The invention discloses a method for directionally inducing and differentiating pig pluripotent stem cells into male germ cells and a special culture medium. The invention provides the special culture medium. The special culture medium comprises an EpiLC inducing medium, a PGCLC (primordial germ cell-like cell) inducing medium and an SSCLC (spermatogonial stem cell-like cell) inducing medium. The invention provides the method for directionally inducing and differentiating the pig pluripotent stem cells into PGCLC in vitro; experiments prove that the PGCLC can be further induced and differentiated to form SSCLC; in vivo, induced cells are injected into seminiferous tubules of mice of which endogenous sperms are removed, and the PGCLC and the SSCLC can survive and are further developed. Therefore, the invention provides a feasible and useful scheme for directional differentiation from the pig pluripotent stem cells to the germ cells, and lays a foundation for exploring a development mechanism and the like of the germ cells.
Owner:CHINA AGRI UNIV
Separation and culture method of testicular mesenchymal stem cells for expressing nidogen and application of testicular mesenchymal stem cells
The invention discloses a separation and culture method of testicular mesenchymal stem cells for expressing nidogen and an application of the testicular mesenchymal stem cells. The separation and culture method comprises the following steps: providing a nidogen-green fluorescent protein transgenic mice model adopting C57BL / 6 as the genetic background; raising the mice for one or more than one week, separating the testis, removing envelopes and blood vessels, exposing the seminiferous tubule, then digesting by using collagenase IV, and filtering digested cell suspension; and sorting by a flow cytometer to obtain single cells and collecting the cells for expressing green fluorescent protein with the fluorescence intensity being 10 times or more than 10 times of the fluorescence intensity of negative control cells so as to separate the testicular mesenchymal stem cells for expressing the nidogen. The invention provides testicular mesenchymal stem / progenitor cells separated according to the preparation and culture method and self-renewed and proliferated cells obtained by culture in a culture medium and provides the application of the cells in preparing a composition for treating related diseases with low testosterone level.
Owner:SUN YAT SEN UNIV
Application of N-acetylcysteine in preparation of drugs for preventing and treating DNA damage and tissue damage induced by synchrotron radiation
The invention relates to an application of an antioxidant drug in the pharmaceutical science field, and especially relates to an application of N-acetylcysteine in preparation of drugs for preventing and treating gonads and other tissue DNA damage and tissue damage induced by synchrotron radiation X-rays. According to experimental results, N-acetylcysteine is indicated to be capable of protecting the double-strand DNA damage caused by synchrotron radiation X-ray radiation, and protecting testicular seminiferous tubule cell layer number reduction caused by the synchrotron radiation X-ray radiation; and the antioxidant is indicated to have important application value in the preparation of the drugs for preventing and treating the synchrotron radiation damage.
Owner:SHANGHAI JIAO TONG UNIV
Anti-breeding rodenticide
InactiveCN103125530AReduce in quantityImprove palatabilityBiocideChemosterilantsInterstitial cellBiology
The invention discloses an anti-breeding rodenticide. Melia azedarach oil is served as an anti-breeding agent, and the weight percent of the melia azedarach oil in the anti-breeding rodenticide accouts for 0.08%-0.1%. Through researching the intrinsic rules and hormone regulation mechanism of mouse breeding as well as studying the toxicological and function mechanism of a vegetable anti-breeding agent, ideal anti-breeding agent-melia azedarach oil is screened. The anti-breeding rodenticide is low in effective dosage, makes mice infertile, and has the advantages of low toxicity index, high safety, no harm to human being and animals and no secondary poisoning. After the anti-breeding rodenticide is eaten 10 days later, main reproductive organs-testicles of male mice generate the following variation: the organ coefficient gets small, the sperm amount is reduced, the seminiferous tubule diameter is thinned, lumens are reduced, germinal epithelium thickness is thinned, and interstitial cells are decreased; and ovarial organ coefficient of female mice is obviously reduced, and the corpus luteum in an ovarial structure is contracted. Dead fetus are increased after male and female mice coition, and the breeding number is greatly decreased.
Owner:XIAN UNVERSITY OF ARTS & SCI
Method for preparing buffalo testis convoluted seminiferous tubule total protein samples and two-dimensional electrophoresis separation method
ActiveCN103951730AQuality improvementHigh resolutionProductsReagentsMuscle tissueConvoluted seminiferous tubules
The invention discloses a method for preparing buffalo testis convoluted seminiferous tubule total protein samples. The method belongs into a lysate extraction-acetone precipitation method and is simple and feasible, fast and reliable and is used in combination with combinative enzymes to effectively digest and remove testis muscle tissue, connective tissue and other tissue components and DNA, and ultimately a plenty of white testis convoluted seminiferous tubules are enriched; the obtained convoluted seminiferous tubules are relatively pure and free of other impurities, the extracted total protein has relatively high quality. The method is suitable for two-dimensional electrophoresis analysis. Accordingly, the inventors establish a set of two-dimensional electrophoresis separation method for buffalo testis convoluted seminiferous tubule total protein samples by trial and error and optimization of a two-dimensional electrophoresis technology system. The electrophoretogram with relatively high quality and relatively high resolution, which can be obtained by the method, offers very important applicable values to the subsequent mass spectrometry and the protein identification.
Owner:GUANGXI UNIV
KNOCK DOWN MODEL OF DICKKOPF HOMOLOGUE 3 (Dkk3) FOR ASSESSING ROLE OF SAID Dkk3 IN SPERMATOGENESIS AND SEX REVERSAL
InactiveUS20120284809A1Increase milk productionIncrease productionMaterial analysisAnimal husbandryDiseaseSpermatogenesis
The present invention relates the function of Dickkopf 3 (Dkk3 ) in testis using shRNA mediated knock down model. Specifically, the present invention provides knock down model comprising reduction in Dkk3 activity. The knock down model of Dkk3 consisting of non human vertebrates which have, incorporated in their genome, shRNA construct targeting mammalian Dkk3 gene exhibits low testis weight, low sperm count and has low litter size. The knock down model displays disrupted seminiferous tubules and are subfertile. The present invention model has a role in sex determination as its interruption leads to sex reversal of XY gonads, converting males to females. Sex reversal role of Dkk3 knock down model can find its utility in agricultural applications. The present invention describes Dkk3 as a dual function protein which is associated with sex determination as well as is essential for the process of spermatogenesis. Such testicular functional studies are useful as a model for various disease states of infertility or subfertility and for identifying a potential treatment to overcome idiopathic infertility.
Owner:NATIONAL INSTUTUTE OF IMMUNOLOGY
Application of LamG3/4/5 of laminin alpha2 functional region in preparation of medicine for preventing contraceptive induced testis injury
PendingCN112516293AAvoid damagePrevent damage/destructionConnective tissue peptidesPeptide/protein ingredientsEpitheliumTestis injury
The invention relates to application of LamG3 / 4 / 5 of a laminin alpha2 functional region in preparation of a medicine for preventing male contraceptive induced testis injury, and belongs to the technical field of medicine preparation. An amino acid sequence of the LamG3 / 4 / 5 of the laminin alpha2 functional region is shown as SEQ ID NO. 1. Overexpression drugs of LamG3 / 4 / 5 of the laminin alpha 2 functional region can effectively prevent testis injury induced by male contraceptives, and can play an obvious role in preventing structural integrity damage of seminiferous epithelium of seminiferous tubules in a testis, lesion of the seminiferous tubules and cytoskeletal protein damage / destruction of the seminiferous epithelium of the seminiferous tubules in the testis which are caused by oral administration of Adjudin.
Owner:THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
Preparation method of buffalo testis single-cell suspension solution
InactiveCN109402046AAvoid mixingIncrease contact areaCell dissociation methodsGerm cellsPhosphateMicroscopic observation
The invention discloses a preparation method of a buffalo testis single-cell suspension solution. The preparation method comprises the following steps: (1) pre-treating raw materials: taking a buffalotestis and disinfecting, then shearing to remove tunica albuginea; washing and cutting into small blocks; (2) digesting: transferring the sheared testis into a culture dish; adding a primary digestive juice for digesting until single seminiferous tubules are obtained; centrifuging and removing supernatant; (3) carrying out primary re-suspension: re-suspending precipitates by utilizing a PBS (Phosphate Buffer Solution); centrifuging and removing supernatant; after repeating for two times, obtaining a bottom-layer re-suspension solution; (4) carrying out cell separation: after carrying out secondary digestion, placing the solution on a microscope and observing; blowing through a pipette to form single cells; after neutralizing, collecting the cells into a centrifugal tube and centrifuging;removing supernatant to obtain a primary cell suspension solution; (5) carrying out secondary re-suspension: re-suspending by utilizing a culture solution and filtering through a cell net to obtain the buffalo testis single-cell suspension solution. The invention provides the preparation method of a buffalo spermatogonial stem cell suspension solution, which has high separation speed and good effect; the method is simple and feasible to operate and is easy to realize.
Owner:卢克焕 +1
Application of canagliflozin and analogs thereof in preparation of medicine for preventing and treating male reproductive dysfunction
ActiveCN113908152AGood treatment effectOrganic active ingredientsSexual disorderAbnormal sperm motilityPhysiology
The invention belongs to the technical field of biomedicines. The invention particularly relates to application of canagliflozin and analogs thereof in preparation of a medicine for preventing and treating male reproductive dysfunction. Research results show that spermatogenic cells at all levels in seminiferous tubules of db / db mice treated by the canagliflozin reappear and are regularly arranged, meanwhile, a small amount of sperms exist in the tubules, and the canagliflozin treatment can be used for significantly improving abnormal sperm quality, abnormal sperm number and abnormal sperm movement of the db / db mice. The canagliflozin and analogs thereof are used for preventing or treating the male reproductive dysfunction for the first time, an ideal treatment effect is achieved, a new prevention and treatment choice is provided for clinical application and treatment of male sterility, especially male sterility caused by diabetes or hyperglycemia, and extremely high application values and social benefits are achieved.
Owner:PEKING UNIV THIRD HOSPITAL
Method for detecting nucleoproteins of spermatogenic cells through utilizing indirect immunofluorescent staining
InactiveCN103235137ARapid expansionProtection maintenanceBiological testingSucroseConvoluted seminiferous tubules
A method for detecting the nucleoproteins of spermatogenic cells through utilizing indirect immunofluorescent staining comprises the following steps: separating convoluted seminiferous tubules from the testis tissues of an animal, utilizing a hypotonic solution to process spermatogenic cells, and detecting the nucleoproteins of the spermatogenic cells through adopting indirect immunofluorescent staining, wherein the hypotonic solution comprises 17mM of sodium citrate, 50mM of sucrose, 5mM of EDTA, 0.5mM of DTT, 0.5mM of PMSF and 30mM of Tris and having a pH value of 8.2. The method uses the hypotonic solution to carry out permeable treatment of the cells in order to rapidly expand the cells, disperse chromatins and fully release the proteins in cell nuclei, so the spermatogenic cells can well combine with primary antibodies, thereby the combination efficiencies of secondary antibodies are enhanced, fluorescent staining is complete and full, and the preparation of spermatogenic cell chromosomes and karyotyping are successfully carried out.
Owner:SHANXI MEDICAL UNIV
Package instrument for obtaining sperms from seminiferous tubule in removed testicular tissue and method
PendingCN111718837AOvercoming easy slidingOvercome immobilityBioreactor/fermenter combinationsBiological substance pretreatmentsTesticleSurgery
The invention belongs to the technical field of instruments for reproductive medicine, and particularly relates to a package instrument capable of improving sperm-obtaining efficiency. The package instrument mainly comprises a fixing device and a poking device; the fixing device comprises a fixing rod, a first connection rod, a second connection rod and a fixing frame; the first connection rod isdetachably connected with the lower portion of the fixing rod, a second connection rod is connected with the lower portion of the first connection rod, and the second connection rod is connected withthe fixing frame; the poking device comprises a tissue poking rod, a third connection rod and a tissue poking device; and the third connection rod is detachably connected with the lower portion of thetissue poking rod, and the tissue poking device is connected with the lower portion of the third connection rod. The package instrument capable of improving the sperm-obtaining efficiency has the beneficial effects that by using the package instrument, testicular tissue can be well fixed, and damage to sperms in traditional sperm-obtaining operation processes and the like is avoided at the same time, so that the probability of success of sperm-obtaining operation is significantly increased.
Owner:MATERNAL & CHILD HEALTH CARE HOSPITAL OF SHANDONG PROVINCE SHANDONG UNIV
Detection method for inhibiting reduction of spermatogenesis of DR5 under oxygen deficit by VHA
ActiveCN111001016ASuppress generationReduce generationCompounds screening/testingSexual disorderIn vitro stimulationLaboratory mouse
The invention belongs to the technical field of inhibition of hypoxia spermatogenesis reduction, and discloses a detection method for inhibition of hypoxia spermatogenesis reduction of DR5 by VHA. VHAgene knockout mice and C57BL / 6 mice are used as research objects, and a hypoxia spermatogenesis reduction model is constructed through hypoxia induction. A seminiferous tubule seminiferous cell of anexperimental mouse is separated to serve as a research object. Hypoxic in-vitro stimulation is conducted, and the regulating effect and the molecular mechanism of VHA for inhibiting seminiferous cellexpression DR5 are verified through western blot, immunocytochemistry and qPCR technologies. According to the detection method for inhibiting DR5 spermatogenesis reduction under oxygen deficit by VHA, which is provided by the invention, the effect of hypoxic inhibition of VHA in hypoxic spermatogenesis reduction and the molecular mechanism of hypoxic inhibition of VHA in inhibition of DR5 expression are clarified, and a necessary theoretical basis and a new treatment strategy are provided for treatment of hypoxic spermatogenesis reduction.
Owner:ARMY MEDICAL UNIV
Testicular tissue cryopreservation method based on single seminiferous tubule perfusion
The invention relates to a testicular tissue cryopreservation method based on single seminiferous tubule perfusion, which comprises the following steps: making testicular tissue into the single seminiferous tubule, soaking a single seminiferous tubule in a cryopreservation protective agent 1 for 10 minutes, and soaking and perfusing the seminiferous tubule by using a cryopreservation protective agent 2, so as to maximally reduce the permeation damage of the protective agent while achieve vitrification cooling, quickly transferring the poured spermatogenic tubule to a Cryo-genic single sperm cryopreservation slice, after sleeving of the Cryo-genic tubule, directly conducting immersing in liquid nitrogen to quickly achieve cooling, and quickly conducting soaking in a recovery solution aftercooling. Compared with a traditional testicular cell suspension cryopreservation method, the method disclosed by the invention has the advantages that (1) the complete structure of the seminiferous tubule is reserved; (2) the oxidative stress level of testicular cells is effectively reduced; (3) the death of a large number of spermatogonial cells and supportive cells after cryopreservation is effectively reduced; and (4) the recovery rate and activity of sperms in the spermatogenic tubules are high.
Owner:SHANGHAI FIRST PEOPLES HOSPITAL
Use of cyanidin-3-O-glucoside and/or protocatechuic acid in improving heat tolerance of testis
PendingCN113768944ADamage reliefRelieve atrophyOrganic active ingredientsAntinoxious agentsMetabolitePhysiology
The invention discloses use of cyanidin-3-O-glucoside (C3G) and / or protocatechuic acid (PCA) in improving heat tolerance of testis. The cyanidin-3-O-glucoside and / or protocatechuic acid can relieve phenomena of atrophy of testis seminiferous tubules, vacuolation of spermatogenic epithelium, reduction of diameter and thickness of testis lumens and the like. The C3G and / or the metabolite PCA thereof can reduce a phosphorylation level of eIF2alpha and an expression of stress particles by reducing an external stress pressure suffered by the testis so as to increase heat tolerance of testis, improve an antioxidant system of the testis and regulate an IRE1alpha-XBP1 pathway so as to relieve oxidative stress and endoplasmic reticulum stress-mediated apoptosis and relieve spermatogenesis disorder and testis injury induced by heat stress. The C3G and / or the metabolite PCA thereof provides a reliable theoretical basis and hasimportant significance for relieving a male reproductive injury induced by the testis in a heat stress state due to bad living habits, occupational factors and pathological reasons in the future.
Owner:JINAN UNIVERSITY
A method for isolation and purification of sheep spermatogonial stem cells, passage for long-term culture, cryopreservation and recovery
ActiveCN104004708BHigh puritySolve the problem of not finding antibodiesDead animal preservationArtificial cell constructsStem Cell IsolationSingle cell suspension
Owner:INNER MONGOLIA UNIVERSITY
Polypeptide with male immunological contraception function and application of polypeptide
InactiveCN107827951ANo damageGood immune contraceptive effectVaccinesContraceptive vaccin ingredientsTesticleMale contraceptive
The invention discloses a polypeptide with a male immunological contraception function and an application of the polypeptide. The amino acid sequence of the polypeptide is as follows: PPRPSSSGYK; andthe polypeptide aiming at a mouse ZNF185 gene is predicted and synthesized through a bioinformatic technology, and after the polypeptide is injected through a seminiferous tubule, a good immunologicalcontraception effect without causing damage to a testis and an epididymis is obtained. The polypeptide provided by the invention provides a novel target for research and development of a male contraceptive vaccine.
Owner:WEIFANG MEDICAL UNIV
Application of swim bladder polypeptide
PendingCN110201140AAntioxidantWith blood pressure loweringHydrolysed protein ingredientsAntinoxious agentsPlant Germ CellsSwim bladder
The invention belongs to the field of biochemical pharmacy and provides application of a swim bladder polypeptide to medicines for inhibiting oxidative damage and cell apoptosis in human bodies or animal bodies. The oxidative damage refer to testicle oxidative damage, the cell apoptosis refers to testicular spermatogenic cell apoptosis, the oxidative damage and the cell apoptosis are caused by antitumor drugs including busulfan, cyclophosphamide and thiotepa, and busulfan is used as the antitumor drug in an embodiment. The swim bladder polypeptide is capable of raising the expression level ofa transcription factor Nrf2, reducing an apoptosis promoting factor Bax and raising the expression level of an anti-apoptosis factor Bcl2; the swim bladder polypeptide is capable of relieving busulfaninduced germ cell vacuolization, spermatogenic cell apoptosis, seminiferous tubule degradation and sperm amount and viability reduction. By adoption of the swim bladder polypeptide for preparation ofthe medicines for inhibiting oxidative damage and cell apoptosis, medicines for recovering fertility of men or male animals can be prepared, and a direction is provided for treatment of fertility ofmen or male animals.
Owner:上海拜汶生物医药股份有限公司
Application of sea grape extract in preparation of pharmaceutical composition or health food for relieving diabetes and preventing or treating reproductive disorder
ActiveCN113491718AImprove atrophyImproved morphological variationMetabolism disorderAlgae medical ingredientsPhysiologyPancreatic hormone
The invention provides application of a sea grape extract in preparation of a pharmaceutical composition or health food. The sea grape extract is obtained by hot water extraction, can improve fasting blood-glucose, insulin concentration in blood and oral glucose tolerance so as to relieve diabetes mellitus, and can improve sperm motility, sperm variation and seminiferous tubule atrophy so as to prevent or treat reproductive disorder, wherein the application mode of the pharmaceutical composition comprises oral administration, soaking, spraying or injection, and the effective dose of the sea grape extract in the human body is 40-100 mg / kg per day.
Owner:NATIONAL TAIWAN OCEAN UNIVERSITY
A technical method for skin stem cells to differentiate into sperm through ectopic development
InactiveCN106754661BReduce harmReasonable configurationCulture processEpidermal cells/skin cellsStainingMitomycin C
Owner:QINGDAO AGRI UNIV
A kind of separation, culture method and application of testicular mesenchymal stem cells
ActiveCN104212763BMammal material medical ingredientsSkeletal/connective tissue cellsCell specificDisease
Owner:SUN YAT SEN UNIV
Transgene method of reverse transcription of transfection primitive spermatogonium abduction in virion
InactiveCN1446918AEasy to operateLow investment costOther foreign material introduction processesFermentationReverse transcriptaseTransgene
A transgenic method using recombinant retrovirus as mediation to transfect stem spermatogonium in body includes cloning target gene to retrovirus carrier, transforming PA317 cells, collecting and concentrating the recombinant virus generated by clone of positive cells, injecting it in the contorted seminiferous tubule of mouse, and reproducing filial generation. Its advantages are simple operation and high repeatability.
Owner:MILITARY VETERINARY INST MILITARY SUPPIES PLA
SCOS (Sertoli cell only syndrome) mouse modeling prejudging method based on Raman spectrum
InactiveCN104730057AOvercome operational complexityOvercoming operating timeRaman scatteringSertoli cell-only syndromePhysics
The invention discloses an SCOS mouse modeling prejudging method based on Raman spectrum, comprising the following steps: a, obtaining mouse sample Raman spectrum feature; b, obtaining Raman spectrum seminiferous tubule spectral peak vector sample database based on the obtained mouse sample Raman spectrum feature; c, obtaining seminiferous tubule Raman spectrum based on the obtained Raman spectrum seminiferous tubule spectral peak vector sample database; d, extracting feature spectral peak based on the obtained seminiferous tubule Raman spectrum; and e, detecting the seminiferous tubule based on the extracted feature spectral peak. The SCOS mouse modeling prejudging method based on Raman spectrum, provided by the invention, can overcome the defects in the prior art, such as complex operation process, long consumed time and low reliability, so as to realize the advantages of simple operation process, short consumed time and high reliability.
Owner:GUANGXI UNIVERSITY OF TECHNOLOGY
Automatic segmentation method for various tissues in mouse testis pathological section based on deep learning
The invention discloses an automatic segmentation method for various tissues in a mouse testis pathological section based on deep learning. The method belongs to the field of machine learning and image processing. The method comprises the following specific steps: pretreating mouse testis cross section slices; establishing a mouse seminiferous tubule segmentation model based on ResNet; and establishing Unet-based segmentation of multiple types of germ cells and multiple types of tissue regions in the mouse seminiferous tubule. Firstly, seminiferous tubule pre-segmentation performedon a mouse testis cross section full-scanning image by using ResNet in combination with a sliding window and a pixel-by-pixel segmentation method; then, multiple types of cell nucleuses and multiple types of tissue regions in the seminiferous tubule are respectively segmented by using Unet. According to the method, good performance is obtained, and a good image analysis basis is provided for establishing a mouse seminiferous tubule automatic staging system; in the future, technicians extract histological characteristics of cell nucleuses and tissue regions in the mouse seminiferous tubule, and the histological characteristics are used for training an automatic staging classifier of the mouse seminiferous tubule.
Owner:NANJING UNIV OF INFORMATION SCI & TECH
A kind of in vitro isolation and culture method of goat male reproductive stem cells
InactiveCN101638633BHas the characteristics of ES cellsStrong shadingTissue cultureHeart Muscle CellFeeder Layer
Owner:NORTHWEST A & F UNIV
A method for isolating, culturing and using testicular mesenchymal stem cells expressing nestin
The invention discloses a method for isolating, culturing and using testicular mesenchymal stem cells expressing nestin. The method of the invention includes: providing a nestin-green fluorescent protein transgenic mouse with C57BL / 6 as the genetic background. Rat model; feed the mice to the age of 1 week or more, separate the testis, remove the capsule and blood vessels, expose the seminiferous tubules and digest with collagenase IV, filter the digested cell suspension; sort by flow cytometry to obtain For a single cell, cells expressing green fluorescent protein whose fluorescence intensity is 10 times or more than that of the negative control cells are collected, thereby isolating the testicular mesenchymal stem cells expressing nestin. The present invention provides Leydig cells derived from testicular stem / progenitor cells isolated according to the above method, as well as self-renewing and proliferating cells obtained by further culturing in culture medium, and provides the use of these cells for the preparation of diseases related to low testosterone levels use of the composition.
Owner:SUN YAT SEN UNIV
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