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Application of sea grape extract in preparation of pharmaceutical composition or health food for relieving diabetes and preventing or treating reproductive disorder

A pharmaceutical composition, technology for reproductive disorders, applied in the direction of drug combinations, functions of food ingredients, medical preparations containing active ingredients, etc., can solve the problems of reproductive disorders without diabetes, achieve oxidation reaction prevention/treatment, alleviate diabetes, The effect of lower environmental harm concerns

Active Publication Date: 2021-10-12
NATIONAL TAIWAN OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no research on sea grapes lowering blood sugar, let alone treating reproductive disorders caused by diabetes

Method used

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  • Application of sea grape extract in preparation of pharmaceutical composition or health food for relieving diabetes and preventing or treating reproductive disorder
  • Application of sea grape extract in preparation of pharmaceutical composition or health food for relieving diabetes and preventing or treating reproductive disorder
  • Application of sea grape extract in preparation of pharmaceutical composition or health food for relieving diabetes and preventing or treating reproductive disorder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, sea grape extract preparation

[0032] After cleaning the fresh sea grape (Caulerpa lentillifera), the algae were ground and crushed and extracted by hot water extraction, and the supernatant was centrifuged and freeze-dried to obtain the sea grape extract (abbreviated as CL); Hot water extraction is carried out in the bacteria kettle, the temperature of hot water extraction is 100-130°C, the better temperature of hot water extraction is 118-124°C; the extraction time of hot water extraction is 45-80 minutes, the better extraction The time is 55-65 minutes, and the extraction pressure of hot water extraction is 1-1.25kg / cm 2 , the optimal extraction pressure is 1.20~1.22kg / cm 2 .

Embodiment 2

[0033] Embodiment two, cell safety test

[0034] The selected cells are Leydig cells (Leydig cell-540). Leydig cells are interstitial cells of the testis, which secrete testosterone and affect the differentiation and maturation of germ cells.

[0035] Two-(1), cell viability test

[0036] Use the MTT colorimetric cell viability test to determine whether the extract is toxic to the cells, culture the cells in a 96-well plate and add different concentrations of sea grape extract (referred to as CL, 1500, 1000, 500, 250 and 125 μg / mL ), cultivated for 24 hours, then added 100 μL of MTT (1 mg / mL) to each well, reacted for 4 hours, added 100 μL of DMSO, oscillated for 10 minutes, and measured the absorbance at 540 nm; Figure 1A From the results, it can be seen that there is no significant difference between different concentrations of extracts by MTT colorimetry, indicating that the sea grape extract is not cytotoxic to Leydig cells.

[0037] Determination of two-(2), nitro blue...

Embodiment 3

[0043] Example 3, Analysis of Diabetic Model Mice

[0044] Six-week-old male BALB / c mice were used as model animals, and the mice were divided into 5 groups (5 mice in each group): mice in the control group were fed commercial feed; mice in the High Fat Diet (HFD) group (20 mice) were fed high-fat diet containing lard, and were injected with streptozotocin (streptozotocin, STZ, 30mg / kg) to induce diabetes, and were divided into 4 treatment groups: diabetes group (Diabetes Mellitus, DM ), positive control group (Metformin (Metformin), 200mg / kg), CL1 group (sea grape extract, 600mg / kg) and CL2 group (sea grape extract, 1000mg / kg), after feeding for 6 weeks and killing, the Tissue samples were stored at -80°C for subsequent biochemical analysis.

[0045] Three-(1), Oral Glucose Tolerance Test (OGTT)

[0046] An oral glucose tolerance test (OGTT) is performed for changes in glucose uptake. Mice were fasted for 10-12 hours the day before the test and were given glucose (2 g / kg B...

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Abstract

The invention provides application of a sea grape extract in preparation of a pharmaceutical composition or health food. The sea grape extract is obtained by hot water extraction, can improve fasting blood-glucose, insulin concentration in blood and oral glucose tolerance so as to relieve diabetes mellitus, and can improve sperm motility, sperm variation and seminiferous tubule atrophy so as to prevent or treat reproductive disorder, wherein the application mode of the pharmaceutical composition comprises oral administration, soaking, spraying or injection, and the effective dose of the sea grape extract in the human body is 40-100 mg / kg per day.

Description

technical field [0001] The invention relates to a sea grape extract, in particular to an application that can relieve diabetes and prevent or treat reproductive disorders. Background technique [0002] Diabetes, known as "diabetes" in ancient times, is an endocrine disease caused by the interaction of genetic and environmental factors. Patients with insufficient insulin secretion or the body's ineffective use of insulin affect the regulation of blood sugar and cause hyperglycemia. [0003] Hyperglycemia is the main clinical sign of diabetes. Due to its high incidence and serious complications, diabetes has become a serious disease that endangers human health after tumors and cardiovascular and cerebrovascular diseases. The absolute or relative insufficient secretion of insulin and the decreased sensitivity of target tissue cells to insulin caused by diabetes can cause a series of metabolic disorder syndromes such as protein, fat, water and electrolyte. If not effectively tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/05A61P3/10A61P15/08A23L33/105
CPCA61K36/05A61P3/10A61P15/08A23L33/105A61K2236/331A23V2002/00A23V2200/328A23V2200/30
Inventor 龚瑞林李孟洲
Owner NATIONAL TAIWAN OCEAN UNIVERSITY
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