Application of nano-micelle-hydrogel preparation of HDAC inhibitor with double targets in medicine for treating acute kidney injury
A technology of acute kidney injury and nano micelles, which is applied in the field of biomedicine to achieve the effects of alleviating renal tubular necrosis, improving renal tubular mesangial sclerosis, and alleviating renal tubular dilation
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Embodiment 1
[0040] Embodiment 1 carries the preparation of SAHA-Ac nano micelles
[0041] 1. Experimental method:
[0042] 1. Prepare a mother solution with a drug concentration of 10 mg / mL, that is, dissolve 22 mg of the drug in 2.2 mL of DMSO. Take the polymer material mPEG-b-PCL (10000:8000) as the carrier, the mass ratio of drug to material is 1:10, add DMSO to dilute to 1 mL, dissolve and mix well under ultrasonic.
[0043] 2. Take another 19mL of ultra-pure water in the sample bottle, add a rod-shaped magnet to the bottle, and then place the sample bottle on a constant temperature electric heating mantle to heat and stir. The assembly temperature was set at 40 °C.
[0044] 3. Use a syringe to take the above mother liquor and add it dropwise to ultrapure water at a rate of 2 seconds per drop. After the addition is complete, continue stirring at 800 rpm for 10 minutes, and finally cool slowly at room temperature.
[0045] 4. Transfer the micellar solution to a 3500Da dialysis bag, ...
Embodiment 2
[0058] Example 2 Preparation and Characterization of Injectable Aseptic Chitosan Thermosensitive Hydrogel
[0059] 1. Experimental method:
[0060] 1. Preparation of chitosan (CS) solution with a mass fraction of 2%: Dissolve 200 mg of CS powder sterilized by high temperature and high pressure (120 ° C, 15 min) in 10 mL of 0.1 M HCl, keep stirring until completely dissolved, and store in an ice bath until use.
[0061] 2. Preparation of 56% sodium β-glycerophosphate (β-GP) solution: 0.56 g of β-GP was dissolved in 1 mL of triple-distilled water, and sterilized by filtration with a 0.22 μm microporous membrane.
[0062] 3. Preparation of drug-loaded micelles hydrogel: add 0.1mL drug-loaded micelles to 0.8mL CS solution, then add 0.1mL β-GP solution, with a volume ratio of CS:β-GP:drug-loaded micelles=8 :1:1 Mix well.
[0063] 4. Concentration and aseptic treatment of the micellar solution: soak the ultrafiltration centrifuge tube with 70% (v / v) isopropanol solution for 15 mi...
Embodiment 3
[0068] Example 3. In vitro inhibition experiment of SAHA-Ac on HDAC2 activation
[0069] 1. Experimental cells:
[0070] HK-2 renal papilloma cell line, placed in RPMI1640 medium with 10% fetal bovine serum, at 37°C, 5% CO 2 Cultured in a constant temperature incubator, subcultured once every two to three days, and the cells that had expired in logarithmic growth were taken for experiments.
[0071] 2. Experimental group:
[0072] 1. Blank group (Control): no drug treatment
[0073] 2. Positive control group (H 2 o 2 1mmol / L): add 1mmol / L H 2 o 2 Activate cells to produce large amounts of p-HDAC2
[0074] 3. Experimental group treated with 5μM SAHA-Ac (H+SAHA-Ac 5μM): 1mmol / L H 2 o 2 +SAHA-Ac 5μM
[0075] 4. Experimental group treated with 5μM SAHA (H+SAHA 5μM): 1mmol / L H 2 o 2 +SAHA 5 μM
[0076] 3. Experimental method
[0077] Add the corresponding reagents to the cells according to the experimental group, and perform protein separation and western blotting ex...
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