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Polypeptide with male immunological contraception function and application of polypeptide

A technology of immune contraception and function, which is applied in the direction of medical preparations containing active ingredients, contraceptive vaccine components, peptides, etc., to achieve a good effect of immune contraception

Inactive Publication Date: 2018-03-23
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional methods of contraception (vasectomy, hormonal contraceptives, condoms, etc.) have limitations

Method used

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  • Polypeptide with male immunological contraception function and application of polypeptide
  • Polypeptide with male immunological contraception function and application of polypeptide
  • Polypeptide with male immunological contraception function and application of polypeptide

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Experimental program
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Effect test

Embodiment 1

[0026] A polypeptide with male immunocontraceptive function, the amino acid sequence of the polypeptide is: PPRPSSSGYK;

[0027] Wherein, the polypeptide contains mouse ZNF185 polypeptide.

[0028] Zinc finger protein (Zincfinger protein, ZNF) 185 is a LIM (Lin-1, Isl-1 and Mec-3) protein, and its N-terminus contains an ATD (actin-targeting-domain) domain that binds to actin. The expression of ZNF185 in testis was significantly higher than that in other tissues, especially in sperm and stromal cells. In addition, the expression of ZNF185 was significantly higher in sexually mature testes than in presexually mature ones. The study found that ZNF185 is an effective target for contraceptive vaccines. The present invention synthesizes a polypeptide with male immune contraceptive function by screening the ZNF185 epitope.

[0029] The preparation method of the above-mentioned polypeptide with male immunocontraceptive function is to predict and determine the ZNF185 antigenic epito...

Embodiment 2

[0038] The application of the above-mentioned polypeptide with male immunocontraceptive function as a contraceptive vaccine.

[0039] The method of using the above-mentioned polypeptide with male immunocontraceptive function is the seminiferous tubule injection immunization method, and the specific operation is: take 10 μg of the polypeptide of the present invention and dilute it to 10 μl, then mix it with 10 μl of Freund’s complete adjuvant, fully emulsify, The seminiferous tubules were injected with the immune body, that is, mice, for the first immunization, and a total of 1 immunization.

[0040] 1. Immunization of male mice after mixing the polypeptide of the present invention with an adjuvant

[0041] 1. Animal grouping

[0042] Twenty-four BALB / c male mice (6-8 weeks old) were divided into 4 groups. Grouping elements: immunization routes include seminiferous tubules injection (Seminiferous tubules injection, IS) and intraperitoneal injection (intraperitoneal injection,...

Embodiment 3

[0084] Detection of the immune contraceptive effect of the polypeptide of the present invention

[0085] 1. Main reagents and their preparation

[0086] ZNF185 polypeptide (Shanghai Jier Biochemical Company);

[0087] BALB / c mice were purchased from Qingdao Daren Fucheng Animal Husbandry Co., Ltd.;

[0088] Complete Freund's Adjuvant (CFA), Incomplete Freund's Adjuvant (IFA) (Sigma, USA);

[0089] 2. Animal grouping

[0090] Twenty-four BALB / c male mice (6-8 weeks old) were divided into 4 groups. Grouping elements: immunization routes include seminiferous tubules injection (Seminiferous tubules injection, IS) and intraperitoneal injection (intraperitoneal injection, IP), immunization dose (10 µg and 80 µg), and adjuvant (as shown in Table 1 above);

[0091] 3. Immune pathway

[0092] (1) Intraperitoneal injection immunization method

[0093] Take 80µg of polypeptide and dilute it to 100µl, then mix it with 100µl of Freund's complete adjuvant, fully emulsify it, and injec...

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Abstract

The invention discloses a polypeptide with a male immunological contraception function and an application of the polypeptide. The amino acid sequence of the polypeptide is as follows: PPRPSSSGYK; andthe polypeptide aiming at a mouse ZNF185 gene is predicted and synthesized through a bioinformatic technology, and after the polypeptide is injected through a seminiferous tubule, a good immunologicalcontraception effect without causing damage to a testis and an epididymis is obtained. The polypeptide provided by the invention provides a novel target for research and development of a male contraceptive vaccine.

Description

technical field [0001] The invention relates to a protein polypeptide vaccine, specifically a polypeptide with male immune contraceptive function and application thereof, which has a certain immune contraceptive effect and belongs to the field of protein polypeptide vaccines. Background technique [0002] The high growth rate of the world's population and the high incidence of unwanted pregnancy have been affecting the socio-economic development and human physical and mental health. Traditional contraceptive methods (vasectomy, hormonal contraceptives, condoms, etc.) all have certain limitations. Therefore, finding a safe, convenient, effective, reversible, non-hormonal contraceptive method is of great significance for controlling population growth and avoiding unwanted pregnancy. In recent years, male contraceptive vaccines have become a research feature, and the research on male contraceptive vaccines is increasing, which may become an ideal measure to control excessive p...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/00A61K39/00A61P15/16
CPCA61K39/0006C07K7/06
Inventor 冯卫国潘智芳樊姝彤赵玉晗潘智卫杨潍潍梁祖穆满怡
Owner WEIFANG MEDICAL UNIV
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