71 results about "Salmonella gloucester" patented technology

The invention provides an attenuated Salmonella vaccine vector comprising one or more heterologous polynucleotides that encode immunogenic Chlamydial peptides. In one embodiment, the attenuated Salmonella vaccine vector comprises aroC and ssaV attenuating mutations. The heterologous polynucleotides encoding the immunogenic Chlamydial peptides can be under the control of an inducible promoter such as a Salmonella ssaG promoter. In one embodiment of the invention, the immunogenic Chlamydial peptide is a PmpG peptide, for instance, a CT110, CT84 or CT40 peptide.

The invention relates to an enrichment culture solution for the detection of Salmonella in poultry eggs, belonging to the technical field of the enrichment culture solution which can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low in poultry eggs. 1L of the enrichment culture solution comprises the following raw material components by weight: 7-11g of peptone, 5-8g of sodium chloride, 7-9g of disodium hydrogen phosphate, 0.5-1g of potassium dihydrogen phosphate, 6-8mg of brilliant green, 0.5-1g of bile salt, 10-14mg of nalidixic acid and 15-40g of skimmed milk powder. The enrichment culture solution can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low; and the enrichment processing steps of Salmonella in foods are reduced, the defect that the leak detection of a small amount of sublethal injured Salmonella in a sample can be overcome and the detection reliability and accuracy of Salmonella in foods can be increased.

The invention discloses a preprocessing method for detecting salmonella live bacteria DNA in meat and meat products. The method comprises the following steps: firstly processing meat and meat products, adding PMA into a processed sample, controlling the final concentration of the PMA to be 15 micrograms / mL, sufficiently and uniformly mixing the mixture, and incubating for 3min in a dark place; illuminating for 3min at the position of 20cm below a 500w halogen tungsten lamp; extracting the genome DNA of the processed sample; and performing real-time fluorescence PCR quantitative detection on salmonella live bacteria in the sample by taking the extracted genome DNA as a template. The method can rapidly detect pathogenic live bacteria in meat and meat product matrix, so that the interference of food matrix turbidity and bacterial pollution background values is avoided, and the detection effectiveness is improved.

The invention discloses a CAMP primer group for amplifying salmonella and a test kit. The CAMP primer group comprises one or more than two selected from the group consisting of the following three primer groups: a primer group for amplifying a CFSA231 complete genome of the salmonella, a primer group for amplifying a CFSA664 complete genome of the salmonella, and a primer group for amplifying a CFSA1096 complete genome of the salmonella. The CAMP primer group is obtained by designing a CFSA231 genome, a CFSA664 genome and a CFSA1096 genome of three specific conserved regions of the salmonella,and the genome of each conserved region is composed of six primers, and comprises a group of sequence main primers (NF / NR), a group of outer primers (F2 / R2) and a group of inner primers (LF / LR). Theprimer group provided by the invention is high in sensitivity and strong in specificity; and the test kit prepared from the primer group can rapidly and accurately detect the salmonella contained in ato-be-detected sample. The visual test kit provided by the invention provides great convenience for on-site detection, and can realize rapid and accurate detection of the salmonella by being preparedinto the test kit.

The invention discloses a feed combination capable of preventing and controlling calf and lamb salmonellosis, which consists of 2-3 parts by weight of coptis root, 1-2 parts by weight of Rhizoma alismatis, 40-44 parts by weight of corns and 55-58 parts by weight of stalks. The preparation method of the feed combination comprises the steps of: processing the 2-3 parts by weight of coptis root, 1-2 parts by weight of Rhizoma alismatis and 40-44 parts by weight of corns into powder with the meshes being 10-40, then processing the 55-58 parts by weight of stalks into particles with length being smaller than 4cm and finally evenly stirring the raw materials. By adding the coptis root and the Rhizoma alismatis into conventional calf and lamb feeds to form the feed combination capable of preventing and controlling the calf and lamb salmonellosis, the raw material compositions, the preparation method and the feeding method of the combination are simple. The combination mainly takes effects of actively preventing the calf and lamb salmonellosis and treating the calf and lamb salmonellosis at an early stage, improving the disease preventing and resisting ability of calves and lambs, obviously reducing the incidence rate of the calf and lamb salmonellosis, reducing the amount of used antibiotics and improving the safety of beef and mutton products eaten by people.

The invention belongs to a selectivity enrichment medium of salmonella and relates to a selectivity enrichment medium which is used for enriching extremely little salmonella with recovery effect for damaged cells in food. The selectivity enrichment medium comprises formula raw materials in parts by weight: 2.5 to 7.5 parts of tryptone, 2.5 to 7.5 parts of fish meal tryptone, 2 to 8 parts of beef extract, 2.5 to 7.5 parts of NaCl, 1 to 2 parts of disodium hydrogen phosphate, 6 to 12 parts of monopotassium phosphate, 1 to 3 parts of mannitol, 5 to 15 parts of sodium pyruvate, 0.2 to 1 part of acarbose, 1.2 to 5 parts of No. 5 cholate, 10 to 30 parts of sodium thiosulfat and 1000 parts of distilled water, wherein the pH is 7.2 to 7.5.

The invention discloses a primer and a kit for detecting salmonella and a use method of the kit, and belongs to the field of bacterial detection. The problems that a salmonella detection method in the prior art is low in detection sensitivity, low in accuracy and high in cost, and false negative and false positive results are likely to occur are solved. The kit disclosed by the invention comprises LAMP (loop-mediated isothermal amplification) primers of a salmonella conserved gene invA, an enteritis serotype specific expression gene prot6E of salmonella enterica, a specific expression gene mdh of salmonella typhimurium, a probe SC-E6 and a probe SC-E6-TM. The kit for detecting salmonella has very high detection accuracy and sensitivity, is convenient to operate and low in cost, and can avoid aerosol pollution in the detection process.

The invention provides a production method of fresh eggs capable of being eaten raw. The production method comprises the two aspects of screening and raising of egg-laying chickens and screening and processing of eggs, wherein screening and raising of the egg-laying chickens comprise raising of chicks into the egg-laying chickens, salmonella detection conducted on the chicks, in different stages,in the raising process, and further raising of the chicks, which are detected to be qualified, into the egg-laying chickens, and screening and processing of the eggs comprise collection, screening, cleaning, air-drying, ultraviolet sterilization, oil applying and packaging treatment conducted on the eggs in a house of the egg-laying chickens. According to the production method of the fresh eggs capable of being eaten raw, salmonella pollution can be controlled starting from a raising environment and a raising source, including drinking water and feed, salmonella detection is conducted on the drinking water, the feed and the raising environment in the whole raising process, and the chicks positive for salmonellae are eliminated, so that the high-quality fresh eggs capable of being eaten raware obtained, salmonella pollution can be avoided, use of antibiotics is reduced, the production method is simple and easy to operate, and the produced eggs have higher quality, and are safer.

The invention relates to a salmonella enteritidis bacteriophage and application thereof, and belongs to the technical field of biology. A strong lytic bacteriophage is characterized in that the collection number of the bacteriophage is GDMCC No: 61171-B1, the bacteriophage is collected in Guangdong Microbiological Culture Collection Center on August 25, 2020, the taxonomic name of the bacteriophage is salmonella enteritidis subspecies, namely salmonella enterica subsp.enterica bacteriophage, and the bacteriophage has an efficient bactericidal ability on salmonella enteritidis.

A nucleic acid molecule utilizable for Salmonella detection is provided. The nucleic acid molecule which binds to Salmonella includes any of the following polynucleotides (a) to (d): (a) a polynucleotide composed of any of base sequences of SEQ ID NOs: 1 to 17; (b) a polynucleotide composed of a base sequence obtained by deletion, substitution, insertion, and / or addition of one or more bases in any of the base sequences in the polynucleotide (a) and is bound to Salmonella; (c) a polynucleotide composed of a base sequence having an identity of 80% or more to any of the base sequences in the polynucleotide (a) and is bound to Salmonella; and (d) a polynucleotide composed of a base sequence complementary to a polynucleotide which hybridizes to the polynucleotide (a) composed of any of the base sequences under stringent conditions and is bound to Salmonella.