72 results about "Salmonella gloucester" patented technology

The invention provides an attenuated Salmonella vaccine vector comprising one or more heterologous polynucleotides that encode immunogenic Chlamydial peptides. In one embodiment, the attenuated Salmonella vaccine vector comprises aroC and ssaV attenuating mutations. The heterologous polynucleotides encoding the immunogenic Chlamydial peptides can be under the control of an inducible promoter such as a Salmonella ssaG promoter. In one embodiment of the invention, the immunogenic Chlamydial peptide is a PmpG peptide, for instance, a CT110, CT84 or CT40 peptide.

The invention relates to an enrichment culture solution for the detection of Salmonella in poultry eggs, belonging to the technical field of the enrichment culture solution which can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low in poultry eggs. 1L of the enrichment culture solution comprises the following raw material components by weight: 7-11g of peptone, 5-8g of sodium chloride, 7-9g of disodium hydrogen phosphate, 0.5-1g of potassium dihydrogen phosphate, 6-8mg of brilliant green, 0.5-1g of bile salt, 10-14mg of nalidixic acid and 15-40g of skimmed milk powder. The enrichment culture solution can realize the selective enrichment of sublethal injured Salmonella of which amount is extremely low; and the enrichment processing steps of Salmonella in foods are reduced, the defect that the leak detection of a small amount of sublethal injured Salmonella in a sample can be overcome and the detection reliability and accuracy of Salmonella in foods can be increased.

The invention discloses a preparation and application of an oral tumor vaccine with attenuated salmonella typhimurium as a vector. The preparation of the vaccine comprises the following steps: (1) firstly, establishing a recombined vaccine vector integrating a micro gene with a self-transfer system through a molecular biological method; and (2) electrically transforming the recombined vaccine vector created in the step (1) into attenuated salmonella typhimurium SL7207 to establish the recombinant oral tumor vaccine. According to the preparation disclosed by the invention, the micro gene and the self-transfer system are integrated onto one vector for the first time, the salmonella typhimurium is electrically transformed to obtain the oral tumor vaccine, and the immune prevention effect of the vaccine on tumors is verified by a mouse tumor model, so that the oral tumor vaccine can be applied in the field of preparing immune preventive medicines for tumors.

The invention discloses a preprocessing method for detecting salmonella live bacteria DNA in meat and meat products. The method comprises the following steps: firstly processing meat and meat products, adding PMA into a processed sample, controlling the final concentration of the PMA to be 15 micrograms / mL, sufficiently and uniformly mixing the mixture, and incubating for 3min in a dark place; illuminating for 3min at the position of 20cm below a 500w halogen tungsten lamp; extracting the genome DNA of the processed sample; and performing real-time fluorescence PCR quantitative detection on salmonella live bacteria in the sample by taking the extracted genome DNA as a template. The method can rapidly detect pathogenic live bacteria in meat and meat product matrix, so that the interference of food matrix turbidity and bacterial pollution background values is avoided, and the detection effectiveness is improved.

The invention discloses a detection method and a detection test strip of salmonella enteritidis. Based on a Gram staining principle, crystal violet is used for staining bacteria into bluish violet firstly; then under the action of capillary effect, the stained bacteria are transferred along a nitrocellulose membrane; based on an antigen and antibody specific binding principle, the bacteria are captured by an antibody of a T line so that the T line becomes a visible strong bluish violet color. The bright bluish violet color can be obtained only if the bacteria are simply stained, and any nano-material labeled antibody is not used so that a complicated labeling process is omitted. According to the detection method and the detection test strip of the salmonella enteritidis, only one antibodyis scratched on the nitrocellulose membrane to detect directly and a traditional sandwich detection method adopting two antibodies is broken through, so that the cost is greatly saved and the difficulty of matching the antibodies is solved; the detection method and the detection test strip are simpler, more convenient and more novel.

The invention provides a salmonella detection primer group, a salmonella detection method and a salmonella detection kit based on an RPA-LbCas12a-TTECDS system, and relates to the field of salmonella detection. Based on the combination of a recombinase polymerase amplification technology and a CRISPR gene detection technology, salmonella is detected through an optimized and modified crRNA double-target gene combination, crRNA is screened and optimized aiming at an invA gene and a fimY gene of the salmonella respectively, and upstream and downstream primers for RPA reaction are designed according to nucleotide sequences at two ends of a matched sequence of the crRNA, so that the primer group is obtained. According to the primer group and the crRNA, the RPA-LbCas12a-TTECDS system is designed, and t hesalmonella detection method and kit are established according to the system. The detection method and the kit provided by the invention are used for detecting actual samples, and have the advantages of strong specificity, rapid detection, high sensitivity and high precision.

The invention provides an attenuated Salmonella vaccine vector comprising one or more heterologous polynucleotides that encode immunogenic Chlamydial peptides. In one embodiment, the attenuated Salmonella vaccine vector comprises aroC and ssaV attenuating mutations. The heterologous polynucleotides encoding the immunogenic Chlamydial peptides can be under the control of an inducible promoter such as a Salmonella ssaG promoter. In one embodiment of the invention, the immunogenic Chlamydial peptide is a PmpG peptide, for instance, a CT110, CT84 or CT40 peptide.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention discloses specific methods such as raw material request, aqueous medium request, ozone water temperature and immersion time, etc. of a technical method for reducing survival percentage of salmonella on the surfaces of eggs by ozone water. The eggs to be processed in the invention are produced by healthy chicken group without salmonella disease and the eggs are fresh eggs with a guarantee period of no more than 7 days. The aqueous medium for preparing the ozone water meets the national NY5028-2001 water quality requirement for processing water of pollution-free food poultry product. When an ozonizer is used for preparing the ozone, the performance of the ozonizer can ensure the ozone concentration in the prepared ozone water to achieve above 4mg / L under the condition of 0 to 30 DEG C. The ozone concentration of ozone water which can reduce the survival percentage of the salmonella on the surfaces of the eggs by above 90 percent is higher than 2mg / L with the optimum temperature of 0 to 5 DEG C. Under the condition of 0 to 30 DEG C, the eggs are soaked in the ozone water with the ozone concentration of above 2mg / L for 10 minutes and then taken out; under the clean condition with the temperature of 20 plus or minus 5 DEG C and the relative humidity of below 30 to 50 percent, the eggs are treated with natural drying or air seasoning and packaged, and then the egg product, the survival percentage of the salmonella on the surface of which being reduced by above 90 percent, is obtained. The methods of the invention can be used for improving the safe mass of the eggs and have broad application value.

The invention discloses a CAMP primer group for amplifying salmonella and a test kit. The CAMP primer group comprises one or more than two selected from the group consisting of the following three primer groups: a primer group for amplifying a CFSA231 complete genome of the salmonella, a primer group for amplifying a CFSA664 complete genome of the salmonella, and a primer group for amplifying a CFSA1096 complete genome of the salmonella. The CAMP primer group is obtained by designing a CFSA231 genome, a CFSA664 genome and a CFSA1096 genome of three specific conserved regions of the salmonella,and the genome of each conserved region is composed of six primers, and comprises a group of sequence main primers (NF / NR), a group of outer primers (F2 / R2) and a group of inner primers (LF / LR). Theprimer group provided by the invention is high in sensitivity and strong in specificity; and the test kit prepared from the primer group can rapidly and accurately detect the salmonella contained in ato-be-detected sample. The visual test kit provided by the invention provides great convenience for on-site detection, and can realize rapid and accurate detection of the salmonella by being preparedinto the test kit.

The invention belongs to the technical field of research on the drug resistance of microorganisms, and provides a siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance effluxpump gene acrA, and a preparation method thereof, specifically a DNA nanometer vector, which has a tetrahedron-like structure formed by assembling an oligonucleotide A single chain, an oligonucleotide B single chain, and an oligonucleotide C single chain. Based on the DNA nanometer vector, the present invention further provides a nanometer system, a preparation method and applications thereof, wherein the DNA nanometer vector loads a siRNA targeting efflux pump gene to from the nanometer system. According to the present invention, the DNA nanometer vector has the tetrahedron-like structure, can stably load siRNA, and can safely, rapidly and efficiently transport the siRNA to target cells so as to inhibit the expression of the Salmonella efflux pump gene, such that the sensitivity on antibiotics by Salmonella is improved so as to effectively inhibit the drug resistance of Salmonella.

The invention relates to a fast check reagent of Salmonella causing food poisoning, in particular to a fast check reagent of food source type Salmonella aertrycke, Salmonella suipestifer, Salmonella enteritidis, TAB vaccine and salmonella B and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum are added in enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria.

The invention belongs to a selectivity enrichment medium of salmonella and relates to a selectivity enrichment medium which is used for enriching extremely little salmonella with recovery effect for damaged cells in food. The selectivity enrichment medium comprises formula raw materials in parts by weight: 2.5 to 7.5 parts of tryptone, 2.5 to 7.5 parts of fish meal tryptone, 2 to 8 parts of beef extract, 2.5 to 7.5 parts of NaCl, 1 to 2 parts of disodium hydrogen phosphate, 6 to 12 parts of monopotassium phosphate, 1 to 3 parts of mannitol, 5 to 15 parts of sodium pyruvate, 0.2 to 1 part of acarbose, 1.2 to 5 parts of No. 5 cholate, 10 to 30 parts of sodium thiosulfat and 1000 parts of distilled water, wherein the pH is 7.2 to 7.5.

The invention relates to a product for preventing and / or treating salmonella infection, which belongs to the technical field of biological medicines. The product provided by the invention can inhibitthe growth of salmonella; the growth amount of salmonella is reduced by 90.4 percent; according to the product disclosed by the invention, the adhesion and invasion capabilities of salmonella can be reduced; the salmonella adhered to and invading HT-29 cells is obviously reduced; moreover, the product provided by the invention can inhibit the expression of salmonella virulence genes; the expression quantities of salmonella virulence genes InvA, AvrA, SsrB, HilA and SopD are respectively reduced by 69.3 percent, 62.4 percent, 57.4 percent, 53.4 percent and 31.3 percent; the inhibition effect isobviously superior to that of single use of ZS2058 or eugenol, so that the product provided by the invention has a strong effect of preventing and / or treating salmonella infection.

The invention discloses a primer for RPA rapid test of salmonella carrying a sulfonamides resistance gene and application of the primer. The primer has better interspecies specificity and intraspecies conservativeness, the technology can detect salmonella and sulfonamides resistance gene (sul3) in one reaction, whether the bacterium is the salmonella carrying the sulfonamides resistance gene (sul3) can be judged in short time, no false positive occurs, expensive instruments are not needed, and accordingly, the primer is more convenient, efficient and accurate. The segments after amplification are different is length and can be distinguished by electrophoresis.

The invention relates to the field of poultry breeding, prevention and treatment and discloses a traditional Chinese medicine composition for treating chicken salmonellosis. The traditional Chinese medicine composition is prepared from, by weight, 600-800 g of poplar flowers, 200-400 g of roots of Chinese pulsatilla, a proper amount of pediococcus acidilactici and a proper amount of bacillus natto. The invention further discloses a chicken feed containing the traditional Chinese medicine composition. The traditional Chinese medicine composition is small in toxicity, free of influences on the human, capable of enhancing the immunity, the metabolism and the absorption ability of chickens, increasing the feed absorption and conversion ratio and improving the vigor of chickens, small in variety of needed Chinese herbs and wide in source; the chicken feed can effectively treat chicken salmonellosis, treat asthenia, white diarrhea, oaritis, spleen swelling and other complications, regulate the intestine and stomach flora of chickens, increase the feed absorption and conversion ratio and improve the vigor of chickens and is remarkable in effect on adult chickens infected with salmonella.

The invention discloses a feed combination capable of preventing and controlling calf and lamb salmonellosis, which consists of 2-3 parts by weight of coptis root, 1-2 parts by weight of Rhizoma alismatis, 40-44 parts by weight of corns and 55-58 parts by weight of stalks. The preparation method of the feed combination comprises the steps of: processing the 2-3 parts by weight of coptis root, 1-2 parts by weight of Rhizoma alismatis and 40-44 parts by weight of corns into powder with the meshes being 10-40, then processing the 55-58 parts by weight of stalks into particles with length being smaller than 4cm and finally evenly stirring the raw materials. By adding the coptis root and the Rhizoma alismatis into conventional calf and lamb feeds to form the feed combination capable of preventing and controlling the calf and lamb salmonellosis, the raw material compositions, the preparation method and the feeding method of the combination are simple. The combination mainly takes effects of actively preventing the calf and lamb salmonellosis and treating the calf and lamb salmonellosis at an early stage, improving the disease preventing and resisting ability of calves and lambs, obviously reducing the incidence rate of the calf and lamb salmonellosis, reducing the amount of used antibiotics and improving the safety of beef and mutton products eaten by people.

The invention discloses salmonella phage SEE-1 and an application thereof, relates to prevention and control of salmonella, and belongs to the field of bioengineering. A novel bacteriophage SEE-1 is separated from a sewage treatment system, the preservation No. is CGMCC NO.1834D, the basic property of genomes is analyzed, and particularly, functional genes and protein are predicted and analyzed. The separated salmonella phage SEE-1 shows quick and efficient splitting efficiency in experiment of splitting the bacterium enteritidis, and can be used for preventing acterium enteritidis or drug resistant acterium enteritidis in the sewage treatment system.

The invention discloses a primer and a kit for detecting salmonella and a use method of the kit, and belongs to the field of bacterial detection. The problems that a salmonella detection method in the prior art is low in detection sensitivity, low in accuracy and high in cost, and false negative and false positive results are likely to occur are solved. The kit disclosed by the invention comprises LAMP (loop-mediated isothermal amplification) primers of a salmonella conserved gene invA, an enteritis serotype specific expression gene prot6E of salmonella enterica, a specific expression gene mdh of salmonella typhimurium, a probe SC-E6 and a probe SC-E6-TM. The kit for detecting salmonella has very high detection accuracy and sensitivity, is convenient to operate and low in cost, and can avoid aerosol pollution in the detection process.

The invention provides a production method of fresh eggs capable of being eaten raw. The production method comprises the two aspects of screening and raising of egg-laying chickens and screening and processing of eggs, wherein screening and raising of the egg-laying chickens comprise raising of chicks into the egg-laying chickens, salmonella detection conducted on the chicks, in different stages,in the raising process, and further raising of the chicks, which are detected to be qualified, into the egg-laying chickens, and screening and processing of the eggs comprise collection, screening, cleaning, air-drying, ultraviolet sterilization, oil applying and packaging treatment conducted on the eggs in a house of the egg-laying chickens. According to the production method of the fresh eggs capable of being eaten raw, salmonella pollution can be controlled starting from a raising environment and a raising source, including drinking water and feed, salmonella detection is conducted on the drinking water, the feed and the raising environment in the whole raising process, and the chicks positive for salmonellae are eliminated, so that the high-quality fresh eggs capable of being eaten raware obtained, salmonella pollution can be avoided, use of antibiotics is reduced, the production method is simple and easy to operate, and the produced eggs have higher quality, and are safer.

The invention relates to a salmonella enteritidis bacteriophage and application thereof, and belongs to the technical field of biology. A strong lytic bacteriophage is characterized in that the collection number of the bacteriophage is GDMCC No: 61171-B1, the bacteriophage is collected in Guangdong Microbiological Culture Collection Center on August 25, 2020, the taxonomic name of the bacteriophage is salmonella enteritidis subspecies, namely salmonella enterica subsp.enterica bacteriophage, and the bacteriophage has an efficient bactericidal ability on salmonella enteritidis.

The invention relates to the technical field of detection, in particular, to salmonella specific forward and reverse primers and probe, a detection kit and an application of the detection kit. The detection kit includes the forward and reverse primers, the probe and a lateral flow chromatography test strip; the 5' end of the reverse primer is labeled by using biotin; and the 5' end of the probe islabeled by using fluorescein isothiocyanate (FITC), a C base in the middle region (at least 30 bp away from the 5' end and at least 15 bp away from the 3' end) is replaced by using [THF[, and the 3'end is end-capped by using C3-spacer. The detection kit can realize real-time on-site immediate and quick field detection by using the isothermal recombinase polymerase amplification and the lateral flow strip (RPA-LFS) method in the non-laboratory environment without relying on instrument equipment.

The invention relates to an application of sophora alopecuroide total alkali in preparation of a traditional Chinese medicine preparation for treating chicken pullorum as well as the traditional Chinese medicine preparation and a preparation method thereof. The traditional Chinese medicine preparation is used for treating salmonella infection of the chicken pullorum. The traditional Chinese medicine preparation consists of the sophora alopecuroide total alkali and auxiliary materials; and the preparation method comprises a step of preparing the sophora alopecuroide total alkali and auxiliary materials into powder or granules by virtue of a preparation process, and the prepared preparation is clinically used for treating the chicken pullorum by virtue of drinking water or stirring. The preparation provided by the invention does not have toxic or side effects and is simple in processing technology and low in production cost, and pharmacodynamics researches show that the preparation provided by the invention has the effects of preventing and treating salmonella of the chicken pullorum, and does not have antibiotic residues after being taken.

The invention relates to an attenuated salmonella of a double expression plasmid which carries Vp3 and TRAIL as well as the applications thereof in constructing and preparing a gene treatment medicine for curing or preventing knub. Not only the body of the invention can restrain the knub from growing, but also can be used as a foreign gene excellent expression carrier to submit the characteristic of a pharmaceutical protein in a knub cell to insert the Vp3 and the TRAIL into the double expression plasmid by combining the tumoraffin of an invasion bacterial with the knub characteristics of the Vp3 and the TRAIL for inducing apoptosis, forms a strain of pBud-Vp3-TRAIL / SL7207 by taking the attenuated salmonella as a carrier and researches the effect and mechanism for resisting the knub inside and outside a body by a mode of gastric cancer. Test inside and outside the body shows that the co-expression of the Vp3 and the TRAIL can play the better coordination effect for restraining the growing of the knub.

A nucleic acid molecule utilizable for Salmonella detection is provided. The nucleic acid molecule which binds to Salmonella includes any of the following polynucleotides (a) to (d): (a) a polynucleotide composed of any of base sequences of SEQ ID NOs: 1 to 17; (b) a polynucleotide composed of a base sequence obtained by deletion, substitution, insertion, and / or addition of one or more bases in any of the base sequences in the polynucleotide (a) and is bound to Salmonella; (c) a polynucleotide composed of a base sequence having an identity of 80% or more to any of the base sequences in the polynucleotide (a) and is bound to Salmonella; and (d) a polynucleotide composed of a base sequence complementary to a polynucleotide which hybridizes to the polynucleotide (a) composed of any of the base sequences under stringent conditions and is bound to Salmonella.

The invention discloses a primer, a kit and a detection method for detecting salmonella at a constant temperature on the basis of polymerase spiral reaction. The invention designs a pair of detectionprimers Ft / Bt and one pair of acceleration primers IF / IB by aiming at the specific region of the specificity target sequence invA conserved region of the salmonella, the nucleotide sequence of the primer is disclosed in SEQ ID NO. 1-4, and the reliability of a detection result is guaranteed by the primer. The invention also provides a kit for detecting the salmonella on the basis of the polymerasespiral reaction. The kit has the characteristics of high sensitivity, good specificity, convenience and high speed in operation, accurate and reliable result and low cost and is suitable for small and medium size departments and field detection application. In addition, the invention also provides a detection method for detecting salmonella at the constant temperature on the basis of the polymerase spiral reaction. According to the method, under a constant temperature condition, short time is consumed, an amplified product does not need gel electrophoresis, and a result can be directly judgedwith eyes after fluorescent dye is directly used for color development.