Construction of attenuation salmonella bacterin with Vp3 and TRAIL co-expression plasmid

Abstract

The invention relates to an attenuated salmonella of a double expression plasmid which carries Vp3 and TRAIL as well as the applications thereof in constructing and preparing a gene treatment medicine for curing or preventing knub. Not only the body of the invention can restrain the knub from growing, but also can be used as a foreign gene excellent expression carrier to submit the characteristic of a pharmaceutical protein in a knub cell to insert the Vp3 and the TRAIL into the double expression plasmid by combining the tumoraffin of an invasion bacterial with the knub characteristics of the Vp3 and the TRAIL for inducing apoptosis, forms a strain of pBud-Vp3-TRAIL / SL7207 by taking the attenuated salmonella as a carrier and researches the effect and mechanism for resisting the knub inside and outside a body by a mode of gastric cancer. Test inside and outside the body shows that the co-expression of the Vp3 and the TRAIL can play the better coordination effect for restraining the growing of the knub.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the construction of an attenuated Salmonella carrying pBud-Vp3-TRAIL-EGFP double expression plasmid and its application in treating tumors. Background technique [0002] For nearly half a century, tumors have become a common disease that seriously threatens human health. According to estimates by the World Health Organization (WHO) and the American Society of Clinical Oncology (ASCO), there are 10 million new cancer patients worldwide every year, and 6-7 million patients die from cancer, accounting for 12% of the deaths. . The fight against cancer has been intensified over the past few decades using surgery, radiation therapy and chemotherapy, but the results have so far been disappointing. Therefore, people continue to explore new methods of tumor treatment. With the rapid development of molecular biology research, the mechanisms of cell growth, differentiation, and apoptosis are...

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Problems solved by technology

Pawelek et al. believe that the substances required by the auxotrophic strains used, such as aromatic amino acids, are very low in normal cells, which is not enough to meet the nutritional needs of bacterial growth, but they are abundant in the "necrotic area" of tumors where cells are constantly dividing. Causes a relative concentration of bacteria; it is also possible that the presence of certain adhesion molecules leads to the targeting of bacteria to tumor cells

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