Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance efflux pump gene acrA, and preparation method thereof

A nano and nano-carrier technology, applied in the field of siRNA-DNA nano system, can solve the problems of short effective time, expensive reagents, long experiment period, etc.

Active Publication Date: 2018-03-16
SICHUAN UNIV
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since naked siRNA is easily degraded, unstable, and has a short effective action time, in order to prolong the action time of siRNA, most of the siRNA silencing bacterial genes are delivered by expression vectors or transfection reagents, but this method has a long experimental period and expensive reagents, which is not feasible. Targeting, requires high concentration of excess siRNA, and easily leads to off-target

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance efflux pump gene acrA, and preparation method thereof
  • siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance efflux pump gene acrA, and preparation method thereof
  • siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance efflux pump gene acrA, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0043] 1. Materials and instruments

[0044] 1.1 Strains

[0045] Chloramphenicol-susceptible Salmonella S1 isolates, chloramphenicol-resistant Salmonella typhimurium R5 isolates with a multidrug-resistant phenotype and positive for RND efflux pump family resistance genes, and chloramphenicol-resistant but negative for associated resistance genes , Salmonella Enteritidis R2 isolates and quality control strain Escherichia coli (E.coli, ATCC 25922) were all preserved by the Sichuan Provincial Key Laboratory of Animal Disease Control and Food Safety, Sichuan University.

[0046] 1.2 Main reagents

[0047] Trizol kit was purchased from Invitrogen;

[0048] Taq enzyme, T4DNA ligase, dNTP, reverse transcription kit RevertAid TM First strand cDNASynthesis Kit was purchased from Fermentas;

[0049] All the DNA sequences designed and used were synthesized by Shanghai Sangon Biotechnology Co., Ltd. by conventional methods in the industry;

[0050] 50×TAE / Mg 2+ Buffer: Weigh 242.2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of research on the drug resistance of microorganisms, and provides a siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance effluxpump gene acrA, and a preparation method thereof, specifically a DNA nanometer vector, which has a tetrahedron-like structure formed by assembling an oligonucleotide A single chain, an oligonucleotide B single chain, and an oligonucleotide C single chain. Based on the DNA nanometer vector, the present invention further provides a nanometer system, a preparation method and applications thereof, wherein the DNA nanometer vector loads a siRNA targeting efflux pump gene to from the nanometer system. According to the present invention, the DNA nanometer vector has the tetrahedron-like structure, can stably load siRNA, and can safely, rapidly and efficiently transport the siRNA to target cells so as to inhibit the expression of the Salmonella efflux pump gene, such that the sensitivity on antibiotics by Salmonella is improved so as to effectively inhibit the drug resistance of Salmonella.

Description

technical field [0001] The invention belongs to the technical field of microbial drug resistance research, and in particular relates to a DNA nanocarrier, an siRNA-DNA nanosystem for targeting and inhibiting Salmonella drug resistance efflux pump gene acrA, a preparation method and application thereof. Background technique [0002] Salmonella is a very important class of Gram-negative bacteria that can cause zoonotic diseases. The problem of Gram-negative bacteria, especially Salmonella antibiotic resistance is increasing, which has become one of the hot spots of global concern. The failure of antibiotic treatment of bacterial infections is increasing due to the continuous spread of multi-drug resistant Gram-negative bacteria. The World Health Organization (WHO) declares that drug-resistant bacteria have become one of the main factors threatening human health, making it more urgent and meaningful to find ways to solve bacterial resistance. Studies have shown that efflux pu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/87C12N1/21C12N15/113C12R1/42
CPCC07K14/255C12N15/113C12N15/87C12N2310/141
Inventor 王红宁张安云张鹏雷昌伟
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com