55 results about "Microbial drug resistance" patented technology

The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.

The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.

The invention relates to preparation method of antibacterial finishing agent of textile apply, belonging to textile assisting agent field. The antibacterial finishing agent for textile apply is prepared from zircoaluminate coupling agent, nanometer titanium dioxide, quaternary ammonium monomer, deionized water, and polymerization initiator synthetically react prepared; by mass, 7-11 parts of zircoaluminate coupling agent, 13-18 parts of nanometer titanium dioxide, 27-39 parts of quaternary ammonium monomer, 117-153 parts of deionized water, 0.8-1.3 parts of polymerization initiator. The method adopts the step of quaternary ammonium monomer after modification nanometer titanium dioxide ion surface grafting, restrict controls quaternary ammonium in basal body, compounds inorganic and organic antibacterial agent, overcomes the weaknesses that nanometer titanium dioxide light touch media loses antibacterial property at dim light and quaternary ammonium safety property appears poor and creates micro organism agent resistance, the finishing agent chemical stability stays poor, tolerates heat poor and more related weaknesses, helps achieves antibacterial agent safety, persistence, and efficiency property.

The invention discloses a method for quickly detecting microbial drug resistance and a special microfluidic chip. The microfluidic chip is composed of a polydimethylsiloxane layer, an agarose layer and a transparent board which are orderly overlapped; two through holes are formed in the polydimethylsiloxane layer; the agarose layer is composed of the agarose and the microorganism and supported by the transparent board. The in-situ cultivation, gradient exposure and real-time observation of the microorganism can be realized by the microfluidic chip; the structure and operation are simple, the microfluidic chip can be quickly prepared and checked and is conveniently used for the ordinary biology lab; the injection pump is not needed; the microorganism does not need the fluorescence and the common microorganism is used; the key part of the chip can be used repeatedly, the cost is low and the special microfluidic chip is suitable for daily operation. The growing status of the microorganism in the medicines different in concentration can be observed in real time and the inhibition characters to the microorganism from the medicines can be quickly obtained; the minimum inhibition concentration (MIC) value, the half inhibitory concentration IC50 and the whole inhibition curve are achieved.

The invention discloses a compound sodium sulfadimidine injection liquid for a pig and a preparation method thereof, aiming at providing a compound kanamycin sulfate injection liquid which has fast effect for treating toxoplasmosis of the pig, addresses both the symptoms and root causes, reduces the time and dosage of used drug and has convenient drug usage, and a preparation method with simple technique and easy implementation. Each 100L injection liquid comprises: 5 to 20 sodium sulfadimidine, 1 to 10kg of lincomycin hydrochloride, 5 to 20kg of doxofylline, 1 to 4kg of trimethoprim, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na, 40kg of propylene glycol, 10kg of 95% alcohol and the balance of water for injection. The injection liquid has fast effect for toxoplasmosis of the pig, addresses both the symptoms and root causes, can effectively treat and prevent secondary infection of bacterium, reduces drug resistance of pathogenic microorganism and leads the pig to accelerate restoring. Simultaneously, the compound sodium sulfadimidine injection liquid is an injection liquid and has convenient use.

The invention provides a reaction screening porous plate with reagent-pre-storing micro-beads, a preparation method and a use method thereof. According to the porous plate, the container of the porousplate is filled with a first liquid, reagent-loading micro-beads are pre-introduced into the bottom portion, the reagent-loading micro-beads can be solid microspheres, liquid micro-droplets, or any combination of solid microspheres and liquid micro-droplets, the first liquid is immiscible with the reagent-loading micro-beads, the porous plate is subjected to film sealing storage, and the reagentpreparation and the reagent adding and screening are not required by users during the use. According to the present invention, with the reagent-pre-storing micro-beads, the consumption of the reagentand the consumption of the sample can be substantially reduced, the mixing reaction with the subsequently-introduced trace sample can be conveniently performed, and the volume of the conventional screening reaction can be reduced by 2-3 orders of magnitude; and the reaction screening porous plate can be widely used for protein crystallization screening, drug interaction screening, microbial drug-resistance screening, culture condition screening, array PCR, single cell analysis and other fields.

The invention discloses a pathogenic microorganism metagenome detection method based on third-generation sequencing, the method comprises the following steps: acquiring original gene detection data of third-generation sequencing of a sample, removing interference sequences, and retaining non-human data; establishing a mapping relationship between the whole species nucleic acid database and the whole species classification database; removing invalid mapping relationships in the mapping relationship set to obtain an effective mapping relationship set of the non-human source data and the whole species classification database, and performing calculation according to the effective mapping relationship set to obtain a species identification result; constructing a microorganism annotation database to obtain microorganism annotation information; constructing a microbial drug resistance database to obtain microbial drug resistance information; and obtaining a microorganism detection report according to the species identification result, the microorganism annotation information and the microorganism drug resistance information. According to the method, the non-human source data is established, the mapping result is optimized, the detection precision is improved, the accuracy of species classification in similar regions is improved, and then species are obtained through prediction and comparison of sequence results.

The invention discloses an antibiotic-free piggy conservation feed and a preparation method thereof. The feed is prepared from the following components of first-class puffed corn, first-class corn, soybean meal, puffed soybean meal, puffed soybean flour, all purpose flour, fermented soybean meal, anchoveta powder, calcium hydrogen phosphate, a suckling pig compound premix, composite acid, composite immunity polysaccharide powder, choline chloride, lysine, methionine, threonine, tryptophan, table salt, baking soda, a compound enzyme preparation, a microecological preparation and stone powder. The antibiotic-free piggy conservation feed is free from any antibiotics, free from drug tolerance for microorganisms, free from antibiotic residues and free from environmental pollution, and achievesthe level of an antibiotic piggy conservation feed in the prior art in the respects of controlling the diarrhoea of piggies and increasing the growing performance of the piggies.

The invention discloses an application of a carbendazim and galangin compound bactericide in citrus rot prevention and fresh keeping. The application comprises the following steps: (1) preparing a carbendazim mother solution: weighing carbendazim powder, and diluting the powder with sterile water to obtain the carbendazim mother solution; (2) preparing a galangin mother solution: weighing galangin, adding dimethyl sulfoxide for dissolution, and adding sterile water to a fixed volume to obtain the galangin mother solution; (3) preparing the carbendazim and galangin compound bactericide: mixingthe carbendazim mother solution with the galangin mother solution according to an equal volume proportion to obtain the carbendazim and galangin compound bactericide; and (4) applying the carbendazimand galangin compound bactericide on the surface of citrus. The bactericide is simple in method and convenient to use, can alleviate the problem of microbial drug resistance caused by long-term use ofa single drug, reduces the use dosage of current bactericides, and is greener and pollution-free. The bactericide effectively inhibits penicillium disease after pickling citrus, and prolongs the preservation period of picked citrus.

The invention discloses an application of a conjugated polymer PFP-G2. The polymer shown in formula I is applied to one of the following situations: (1) preparing a product capable of causing the concentration variation of signal molecules released by a microorganism; (2) preparing a product capable of prolonging the time for the microorganism to release the signal molecules; (3) preparing a product capable of causing the variation of drug resistance of the microorganism; (4) preparing a product for detecting concentration of the signal molecules released by the microorganisms; the microorganism can generate the signal molecules and respond to the signal molecules. By utilizing the electrostatic action and hydrophobic action between the conjugated polymer and bacteria, a novel conjugated polymer-bacterial composite group is established in a self-assembling method. By utilizing the phenomenon that vibrio harveyi responds to the external signal molecules and the light of vibrio harveyi is enhanced, the variation of the signal molecular concentration is detected, so that the variation of the bacteria concentration is reflected, and the variation of the bacteria drug resistance can be reflected. (See specifications).

The invention discloses a compound kanamycin sulfate injection liquid for a pig and a preparation method thereof, aiming at providing a compound kanamycin sulfate injection liquid which has fast effect for treating pant disease of the pig, addresses both the symptoms and root causes, reduces the time and dosage of taking drug and has convenient drug usage, and a preparation method with simple technique and easy implementation. Each 100L injection liquid comprises: 5 to 20 billion units of kanamycin monosulfate, 1 to 10kg of lincomycin hydrochloride, 0.1 to 2kg of doxofylline, 0.05kg of dexamethasone sodium phosphate, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na, 10kg of dimethyl acetamide and the balance of water for injection. The injection liquid reasonably mixes usage of all the components by a plurality of types of drugs, has fast effect for treating pant disease of the pig, addresses both the symptoms and root causes, reduces drug resistance of pathogenic microorganism and leads the pig to accelerate restoring. Simultaneously, the compound kanamycin sulfate injection liquid has convenient use, and can achieve the treatment effect of a plurality of types of drugs.

The invention relates to the technical field of medical materials, in particular to a water-based polyurethane liquid dressing and a preparation method thereof and an antibacterial film layer. According to the invention, a certain amount of hydramine, hydroxymethyl carboxylic acid and sodium sulfonate are introduced as hydrophilic chain extenders in a process of preparing a polyurethane prepolymer, so that the polyurethane prepolymer can be subjected to proper hydrophilic modification, and can be used as a film forming substance of the water-based liquid dressing; by introducing a certain amount of an antibacterial agent capable of chemically reacting with polyisocyanate, the antibacterial agent is introduced into a polymer chain segment in a chemical grafting manner and is not easy to lose into the environment to cause microbial drug resistance, and the long-term antibacterial effect of the dressing is realized; and the introduction of the antibacterial agent also improves the crosslinking density of polyurethane to a certain extent, and maintains thecrosslinking density within a reasonable range, so that the dressing has certain water resistance after film formation while the dressing also has relatively good air permeability.

The invention belongs to the technical field of research on the drug resistance of microorganisms, and provides a siRNA-DNA nanometer system for targetedly inhibiting Salmonella drug-resistance effluxpump gene acrA, and a preparation method thereof, specifically a DNA nanometer vector, which has a tetrahedron-like structure formed by assembling an oligonucleotide A single chain, an oligonucleotide B single chain, and an oligonucleotide C single chain. Based on the DNA nanometer vector, the present invention further provides a nanometer system, a preparation method and applications thereof, wherein the DNA nanometer vector loads a siRNA targeting efflux pump gene to from the nanometer system. According to the present invention, the DNA nanometer vector has the tetrahedron-like structure, can stably load siRNA, and can safely, rapidly and efficiently transport the siRNA to target cells so as to inhibit the expression of the Salmonella efflux pump gene, such that the sensitivity on antibiotics by Salmonella is improved so as to effectively inhibit the drug resistance of Salmonella.

The invention relates to a cephalosporin compound and a preparation method thereof. Methoxyl cephalosporin is a cephalosporin derivative where alpha-methoxyl is introduced to position 7. As the methoxyl has the steric hindrance effect to prevent enzyme molecules from approaching beta-lactam ring, so that the stability of the medicine to beta-lactamase is increased, and the activity to anaerobion is improved. In recent years, antibiotic resistive phenomena often occur to affect use of some antibiotics in main categories, so that development of the antibiotic market is directly restricted. Therefore, research of novel cephalosporin compounds is necessary. The cephalosporin compound has the structural formula (I). The invention further relates to the preparation method of the cephalosporin compound and a preparation method of intermediates in the preparation process. The invention provides the cephalosporin compound and the preparation method thereof as well as a novel cephalosporin compound and the preparation method thereof. The cephalosporin compound can be used as an ideal substitutive pharmaceutical compound for conventional antibiotics and antibiotics with microbial resistance.

The invention discloses a method for detecting drug-resistant genes of pathogenic microorganisms of a human body. The method comprises the steps that drug-resistant genes, endogenous standard genes and exogenous standard genes are determined; multiple amplification primers are prepared; exogenous nucleic acid is added to a sample to be detected, a mixed sample is obtained, and a genome is extracted; the exogenous standard genes are added to the genome, and mixed nucleic acid is obtained; the mixed nucleic acid is amplified, the amplification product is utilized for constructing a high-throughput sequencing library, high-throughput sequencing is carried out, and a sequencing fragment set is obtained; the sequencing fragment set is analyzed, and whether an experiment succeeds or not is judged according to the number of the obtained sequencing fragments of the exogenous standard genes and the endogenous standard genes; if the experiment succeeds, the content of the drug-resistant genes is calculated, and whether the sample to be detected contains the drug-resistant genes or not is judged according to the content of the drug-resistant genes. According to the method, any multiple drug-resistant genes expected to be detected in any microorganism can be quantitatively detected at a time, the microorganisms do not need to be cultured or purified during detection, the speed is high, and the result is accurate.

The present invention relates to compositions comprising thioridazine, and derivatives thereof, together with antibacterials. These compositions have been found greatly enhance the activity of many classes of antibacterials, allowing the antibacterials to be administered at significantly lower doses. The thioridazine and the antibacterial act synergistically by pacifying the bacteria to the antibacterials. This synergistic affect lowering the inhibitory or effective concentration of the antimicrobials is most pronounced with the levorotatory isomer of thioridazine compared to the racemic or dextrorotatory isomer.

The invention discloses a microorganism drug resistance gene detection method. The method includes the steps that a drug resistance gene, an endogenous standard gene and an exogenous standard gene are determined; multiple amplification primers are prepared; exogenous nucleic acid is added into a sample to be detected to obtain a mixed sample and extract a genome; the exogenous standard gene is added into the genome to obtain mixed nucleic acid; the mixed nucleic acid is amplified, a high-throughput sequencing library is constructed through amplification productimers, and high-throughput sequencing is conducted to obtain a sequencing fragment set; the sequencing fragment set is analyzed, and whether an experiment succeeds or not is judged according to the number of sequencing fragments of the obtained exogenous standard gene and the endogenous standard gene; if the experiment succeeds, the content of the drug resistance gene is calculated; according to the content of the drug resistance gene, whether the sample to be detected contains the drug resistance gene or not is judged. By means of the method, various drug resistance genes needing to be detected in any microorganism can be detected quantitatively at a time. Besides, microorganisms do not need to be cultured or purified during detection, the speed is high, and results are accurate and reliable.

The invention discloses siRNA for inhibition of microorganism drug resistance and an application of same. The siRNA, when being introduced into a drug-resistance bacterial strain, can silence and interfere in capture of a drug-resistance gene cassette by an integrase gene in an integron system and expression of a drug-resistance gene. Through RT-PCR quantitative detection on change of expression level of mRNA of the integrase gene, detection on microorganism drug resistance, detection on integration efficiency of the integrase and the like, interference efficiency on the siRNA is evaluated, and finally, the siRNA can control and eliminate the drug resistance of pathogenic microorganisms due to the integron. The siRNA can highly-effectively and specifically interfere in the expression of the integrase and the drug-resistance gene so as to inhibit the drug resistance of bacteria, thereby developing a new way for development of novel medicines.

The invention relates to the field of anti-corrosion coatings, in particular to a sulfur ion-responsive nano container and its application as an intelligent protective coating material for microbial corrosion (sulfate-reducing bacteria corrosion) and a preparation method for the coating material. With hollow mesoporous silica nanoparticles (HMSNs) as the core and 2-methylimidazolium zinc salt (ZIF-8) as the shell (nano-valve), the bactericide is loaded inside the cavity of the HMSNs to obtain the target nano-container. The invention is based on the microbial corrosion intelligent protective coating of the sulfur ion responsive nano container, which can actively respond to the change of the sulfur ion concentration in the microenvironment caused by sulfate reducing bacteria, and realize the fixed-point, timing and quantitative control release of the fungicide, and the released sterilization The antibacterial agent plays the role of sterilization and microbial corrosion protection, avoiding the shortening of the service life of the coating, the increase of cost, and the environmental problems and microbial resistance problems caused by the excessive release of antimicrobial agents due to the spontaneous leakage of antimicrobial agents.

The invention discloses a bacteriostatic composition of sweet potato leaf chlorogenic acid and nano zinc oxide, and belongs to the field of traditional Chinese medicine. Combined use of two drugs of a chlorogenic acid extraction liquid and nano zinc oxide in the bacteriostatic composition plays the synergistic effect on inhibition of escherichia coli O157:H7 and staphylococcus aureus. Compared with the prior art, the bacteriostatic composition of the sweet potato leaf chlorogenic acid extract and nano zinc oxide solves the problem of larger drug using amount in a conventional bacteriostatic method, and at the same time, the bacteriostatic composition adopting the two low-dosage bacteriostatic substances decreases the occurrence probability of microbial drug resistance, reduces the toxicity to human bodies, and is safer.

A sample group forming section 24 classifies samples derived from microorganisms into groups according to empirical information showing the species or strain of each sample. A differential analysis section 27 performs a differential analysis using a peak matrix created based on the result of the grouping. An operator enters group rearrangement conditions concerning the drug resistance of microorganisms. Under the entered conditions, a sample group rearranging / rearrangement-cancelling section 25 rearranges the already formed groups by selecting or merging groups using another kind of previously registered empirical information which shows the drug resistance of each group. The differential analysis section 27 performs a differential analysis using a peak matrix newly created based on the result of the rearrangement of the groups. Thus, differential analysis results concerning the resistance to different drugs can be sequentially acquired as the group rearrangement condition is successively changed.

The invention discloses a method for quickly detecting microbial drug resistance and a special microfluidic chip. The microfluidic chip is composed of a polydimethylsiloxane layer, an agarose layer and a transparent board which are orderly overlapped; two through holes are formed in the polydimethylsiloxane layer; the agarose layer is composed of the agarose and the microorganism and supported by the transparent board. The in-situ cultivation, gradient exposure and real-time observation of the microorganism can be realized by the microfluidic chip; the structure and operation are simple, the microfluidic chip can be quickly prepared and checked and is conveniently used for the ordinary biology lab; the injection pump is not needed; the microorganism does not need the fluorescence and the common microorganism is used; the key part of the chip can be used repeatedly, the cost is low and the special microfluidic chip is suitable for daily operation. The growing status of the microorganism in the medicines different in concentration can be observed in real time and the inhibition characters to the microorganism from the medicines can be quickly obtained; the minimum inhibition concentration (MIC) value, the half inhibitory concentration IC50 and the whole inhibition curve are achieved.

The invention discloses a method for detecting drug resistance genes of soil microorganisms. The method comprises the following steps: determining drug resistance genes, standard endogenous genes and standard exogenous genes; preparing multiple amplification primers; adding exogenous nucleic acid into a to-be-tested sample, so as to obtain a mixed sample, and extracting a genome; adding the standard exogenous genes into the genome, so as to obtain mixed nucleic acid; amplifying mixed nucleic acid, constructing a high-throughput sequencing library by virtue of amplification products, and carrying out high-throughput sequencing, so as to obtain a sequencing segment group; analyzing the sequencing segment group, and judging whether the experiment is successful or not according to the number of obtained sequencing segments of the standard exogenous genes and the standard endogenous genes; if the experiment is successful, calculating the content of the resistance genes; and judging whether the to-be-tested sample contains the resistance genes according to the content of the resistance genes. By virtue of the method, a number of arbitrary drug resistance genes, which are expected to be detected, in arbitrary microorganism can be quantitatively detected once, furthermore, the microorganisms do not need to be cultured and purified during the detection, so that the detection speed is high, and the detection result is accurate and reliable.

The invention provides a veterinary herbal medicine composition which is prepared from codonopsis pilosula, rhizoma atractylodis, hawthorn, astragalus, herba epimedii, radix cynanchi bungei, malt, red ochre, sulfur, cyrtomium fortune, selenium yeast, monensin and zeolite powder. The invention also provides a preparation method. The preparation method comprises the following steps: mixing codonopsis pilosula, rhizoma atractylodis, hawthorn, astragalus, herba epimedii, radix cynanchi bungei, maltand cyrtomium fortune and crushing and drying the mixture; and mixing the mixture with red ochre powder which is calcined and quenched by vinegar and crushed sulfur powder. The invention also provides application. The veterinary herbal medicine composition is added into basic ration for gaining weight of cattle. The veterinary herbal medicine has the functions of tonifying middle-Jiao and Qi, tonifying spleen and appetizing, warming the kidney and supporting Yang, detoxifying and killing worms and the like, plays roles of improving the internal environment of body, regulating effective microbial community of intestinal tract, promoting growth of cattle effectively and the like, and solves the problem of decrease of resistance, deficiency-cold in spleen and stomach and immunological paralysis of cattle, microbial tolerance and residues of drugs and heavy metals induced by high density breeding and environmental dankness in modern cultivation.