The invention belongs to the technical field of research on the
drug resistance of microorganisms, and provides a siRNA-
DNA nanometer
system for targetedly inhibiting
Salmonella drug-resistance effluxpump
gene acrA, and a preparation method thereof, specifically
a DNA nanometer vector, which has a
tetrahedron-like structure formed by assembling an
oligonucleotide A
single chain, an
oligonucleotide B
single chain, and an
oligonucleotide C
single chain. Based on the
DNA nanometer vector, the present invention further provides a nanometer
system, a preparation method and applications thereof, wherein the
DNA nanometer vector loads a siRNA targeting
efflux pump
gene to from the nanometer
system. According to the present invention, the DNA nanometer vector has the
tetrahedron-like structure, can stably load siRNA, and can safely, rapidly and efficiently transport the siRNA to target cells so as to inhibit the expression of the
Salmonella efflux pump
gene, such that the sensitivity on
antibiotics by
Salmonella is improved so as to effectively inhibit the
drug resistance of Salmonella.