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Bacteriostatic composition of sweet potato leaf chlorogenic acid and nano zinc oxide

A technology of nano-zinc oxide and chlorogenic acid, which is applied in organic chemistry, antibacterial drugs, inorganic active ingredients, etc., can solve the problems of affecting human health and large dosage at one time, so as to reduce dosage, enhance antibacterial performance, increase The effect of solubility

Inactive Publication Date: 2014-08-13
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Concentrated Chinese medicine preparations made into oral liquids or antibacterial compositions have a large dosage at one time, and long-term use will affect human health

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. The preparation of chlorogenic acid extract comprises the following steps: accurately weigh 6g of sweet potato leaf powder, add 60% ethanol solution with a pH value of 4 according to the ratio of solid to liquid 1g:12mL; Heat suction filtration, repeat the filter residue once, then reflux extraction for 1 hour, suction filtration, and combine the filtrates. According to the proportion of about 2%, add activated carbon for decolorization in 2-3 times, each decolorization 30min after suction filtration, the filtrate is centrifuged at 5000r / min for 30min, and the supernatant is collected for later use. Chlorogenic acid was standardized and quantified in the obtained extract by an ultraviolet-visible photometer. The content of chlorogenic acid in the extract was 47.8μg / mL.

[0015] 2. The nano-zinc oxide solution is prepared according to the following method: Take 0.01g of nano-zinc oxide produced by Shanghai Aladdin Co., Ltd. and dissolve it in 1mL of distilled water, ...

Embodiment 2

[0027] 1, the preparation process of chlorogenic acid extract: with embodiment 1

[0028] 2, the preparation process of nano zinc oxide: with embodiment 1

[0029] 3. Synergistic antibacterial effect of chlorogenic acid extract and nano zinc oxide

[0030] a Culture of bacteria

[0031] Pick a single colony of Staphylococcus aureus on LB agar medium and place it in 10 mL of LB broth medium, culture it in a 37°C incubator for 12 h, and then inoculate 5 mL of LB broth according to the inoculum amount of 1%. In the culture medium, after culturing at 37°C for 5 hours, take 1 mL of the above bacterial solution and serially dilute two gradients with 1xPBS water, and the final bacterial solution is about 10 6 CFU / mL.

[0032] b Synergistic effect of chlorogenic acid and nano-zinc oxide on antibacterial properties

[0033] Take 1 mL of the above-mentioned diluted bacterial solution into 1.5 mL sterilized centrifuge tubes, and add 50 μL of chlorogenic acid extract to No. 1 centrifu...

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Abstract

The invention discloses a bacteriostatic composition of sweet potato leaf chlorogenic acid and nano zinc oxide, and belongs to the field of traditional Chinese medicine. Combined use of two drugs of a chlorogenic acid extraction liquid and nano zinc oxide in the bacteriostatic composition plays the synergistic effect on inhibition of escherichia coli O157:H7 and staphylococcus aureus. Compared with the prior art, the bacteriostatic composition of the sweet potato leaf chlorogenic acid extract and nano zinc oxide solves the problem of larger drug using amount in a conventional bacteriostatic method, and at the same time, the bacteriostatic composition adopting the two low-dosage bacteriostatic substances decreases the occurrence probability of microbial drug resistance, reduces the toxicity to human bodies, and is safer.

Description

Technical field [0001] The present invention belongs to the field of traditional Chinese medicine, and especially involves a traditional Chinese medicine. Background technique [0002] Sweet potato, also known as sweet potato, sweet potato, is the most important food, feed and industrial raw materials in the world, and is widely planted in more than 100 countries.Sweet potato is medicine and food, and has a variety of health functions such as supplementing medium, blood, fertilizer, dirty, qi, qi, spleen and stomach, strong kidney and yin.At the same time, it also has the effects of eliminating active oxygen, inhibiting skin aging, inhibiting cholesterol, preventing emphysema, preventing cancer, and benefiting cardiovascular health.In addition to the above nutrients, sweet potato leaves are also rich in active polysaccharides and chloric acid compounds.Modern pharmacological studies have shown that green acidic acid has antibacterial and antiviral effects.In addition, green origi...

Claims

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Application Information

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IPC IPC(8): A61K33/30A61P31/04A61K31/216C07C69/732
CPCY02A50/30
Inventor 许恒毅况慧娟魏华熊勇华赖卫华
Owner NANCHANG UNIV
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