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Application of conjugated polymer PFP-G2

A polymer, microorganism technology, applied in the field of chemistry, to achieve the effect of regular size

Active Publication Date: 2015-09-23
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic and foreign researchers' work on conjugated polymers is mainly focused on the design and synthesis of new conjugated polymer materials and the establishment of new biological detection and imaging systems, but the application of fluorescent conjugated polymers to bacterial quorum sensing The interaction between quorum sensing and bacterial drug resistance based on conjugated polymers has not been reported

Method used

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  • Application of conjugated polymer PFP-G2
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  • Application of conjugated polymer PFP-G2

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1, growth and luminescence curve of Vibrio harveyi

[0050] (1), recovery culture of Vibrio harveyi

[0051] Using Vibrio harveyi BB170 as a template, use 75% alcohol absorbent cotton to sterilize the outer surface of the purchased ampule bottle containing Vibrio harveyi BB170 in an ultra-clean workbench, heat the top of it with a flame, and drop 300-400 μL of sterile Water to the top of the heated ampoule to rupture it. Draw 300-500 μL of suitable liquid culture medium (can be replaced by sterile water), drop it into the ampoule bottle, shake and blow lightly, so that the freeze-dried bacteria dissolve and form a suspension, absorb all the bacterial suspension, and transplant them into two 2216 cells respectively. In the solid medium of marine bacteria, culture at 30°C for 24 hours. Use an inoculation loop to scrape an appropriate amount of thalli to inoculate into the new 2216 marine bacteria solid medium, and continue to cultivate at 30°C, so that the co...

Embodiment 2

[0058] Example 2. Imaging experiment of Escherichia coli MG1655 and conjugated polymer PFP-G2

[0059] (1), recovery culture of Escherichia coli

[0060] Taking Escherichia coli MG1655 as an example, use 75% alcohol absorbent cotton to sterilize the outer surface of the purchased ampoule containing Escherichia coli MG1655 in the ultra-clean workbench, heat the top of it with a flame, and drop 300-400 μL of sterile water into the heated The tip of the ampoule was broken. Draw 300-500 μL of suitable liquid culture medium (can be replaced by sterile water), drop it into the ampoule, gently shake and blow it, so that the freeze-dried bacteria dissolve into a suspension, absorb all the bacterial suspension, and transplant it into two LB solids In the culture medium, culture at 37° C. at 180 rpm for 12 hours. Use an inoculation loop to scrape an appropriate amount of bacterium and inoculate it into a new LB solid medium, rotate at 180rpm at 37°C and continue to cultivate, so that ...

Embodiment 3

[0066] Example 3, the influence of the PFP-G2 polymer shown in formula I on the signal molecule shown in formula II in Escherichia coli MG1655

[0067] (1), recovery culture of Escherichia coli MG1655

[0068] With the step (1) of embodiment 2.

[0069] (two), the synthesis of PFP-G2 polymer shown in formula I

[0070] With the step (2) of embodiment 2.

[0071] (3), the compound PFP-G2 polymer shown in formula I is introduced in Escherichia coli MG1655

[0072] Pick an appropriate amount of Escherichia coli MG1655 from LB solid medium and place it in LB liquid medium, culture it with shaking at 180rpm at 37°C for 5-10h, adjust the OD with LB medium 600 The value is 1.0. Take a small amount of bacterial solution and dilute it 1000 times with LB medium, add PFP-G2 polymer shown in formula I with a final concentration of 100 μM, and control group does not add PFP-G2. Shake culture at 37°C at 180rpm for 1-8h, and the bacteria The solution was centrifuged at room temperature ...

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Abstract

The invention discloses an application of a conjugated polymer PFP-G2. The polymer shown in formula I is applied to one of the following situations: (1) preparing a product capable of causing the concentration variation of signal molecules released by a microorganism; (2) preparing a product capable of prolonging the time for the microorganism to release the signal molecules; (3) preparing a product capable of causing the variation of drug resistance of the microorganism; (4) preparing a product for detecting concentration of the signal molecules released by the microorganisms; the microorganism can generate the signal molecules and respond to the signal molecules. By utilizing the electrostatic action and hydrophobic action between the conjugated polymer and bacteria, a novel conjugated polymer-bacterial composite group is established in a self-assembling method. By utilizing the phenomenon that vibrio harveyi responds to the external signal molecules and the light of vibrio harveyi is enhanced, the variation of the signal molecular concentration is detected, so that the variation of the bacteria concentration is reflected, and the variation of the bacteria drug resistance can be reflected. (See specifications).

Description

technical field [0001] The invention belongs to the field of chemistry and relates to the application of a conjugated polymer PFP-G2. Background technique [0002] Quorum sensing is a way for microorganisms to adapt to the surrounding environment by secreting and releasing a signal molecule called an autoinducer (AI) and sensing its concentration changes to detect the density of the flora and regulate the physiological functions of the flora. Signal exchange mechanism. The amount of signal molecules released is closely related to the growth density of bacteria, and bacteria can use signal molecules to sense changes in the population density of themselves or other bacteria in the surrounding environment. When the concentration of signal molecules reaches a certain threshold, the signal molecules will enter the interior of bacteria and regulate the expression of certain genes, such as antibiotic production, symbiosis, bioluminescence, regulation of nitrogen fixation genes, sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12R1/19
Inventor 王树张鹏博刘礼兵吕凤婷
Owner INST OF CHEM CHINESE ACAD OF SCI
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