The invention relates to the technical field of biosensors, in particular to a
biosensor for detecting ATP (triphosadenine) activity based on surface-enhancement
Raman scattering generated by nanogoldparticle aggregation. By means of the characteristic that ATP can be specifically combined with a
nucleic acid aptamer of the ATP, Walker Chains of the surfaces of the nanogold particles are released, so that shorter Track Chains on the surfaces of nanogold are catalytically hydrolyzed by means of
nuclease, and therefore, the nanogold particles lose
nucleic acid protection and are aggregated in ahigh salt
buffer solution to generate a surface
plasma resonance effect, thereby enhancing the
electromagnetic field intensity of the surfaces of the nanogold particles greatly. A Raman dye marked onthe surfaces of the nanogold particles generates a surface-enhancement
Raman scattering (SERS) effect and Raman spectra are shown in special positions. When a reaction solution does not contain ATP,Protect Chains cannot be replaced, so that a follow-up nanogold aggregation reaction cannot be carried out, and therefore, no
Raman scattering spectra are generated. The
biosensor detects the ATP activity quickly, sensitively and safely by taking
nucleic acid chains modified on the surfaces of the nanogold particles.