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Automatic classification detecting method for multiple analytes based on surface enhancement Raman scattering technology

A technology of surface-enhanced Raman and classification detection, which is applied in the field of nanometer applications and can solve problems such as interference

Inactive Publication Date: 2015-04-22
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned methods can only be tested for a single ion. When these methods are used in actual systems, there are still many problems
For example, ambient color will greatly interfere with atomic absorption spectroscopy, and organic dark groups and heteroatoms in the substance to be detected will greatly interfere with fluorescence spectroscopy

Method used

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  • Automatic classification detecting method for multiple analytes based on surface enhancement Raman scattering technology
  • Automatic classification detecting method for multiple analytes based on surface enhancement Raman scattering technology
  • Automatic classification detecting method for multiple analytes based on surface enhancement Raman scattering technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] This embodiment prepares nano-gold mixed solution probes according to the following steps:

[0072] 1. Preparation of three kinds of hydrophilic gold nanoparticles mixed solution:

[0073] A: Take a dry anhydrous and anaerobic reaction bottle with a magnetic stirring bar, and add t-BPEA (243mg, 1mmol), DTBE (4.5mg, 0.01mmol) and 4mL DMF prepared in advance to 10mL under nitrogen protection In the reaction flask, degassed with nitrogen for 30min, then added CuCl (0.99mg, 0.01mmol) and Me6TREN (2.3mg, 0.01mmol) to the reaction solution, degassed the mixture with nitrogen for 5min, then stirred the reaction at 25°C Stop at 15h, add 1mL ether to quench, precipitate the polymer, centrifuge at 5000r / min for 10 minutes to obtain a solid product, wash with 4mL ether three times, and vacuum dry until the mass remains constant to obtain P(t-BPEA).

[0074] Take a single-port reaction flask with a magnetic stirrer, dissolve 20mg of P(t-BPEA) in 5mL of THF, slowly add 2mg of LiOH ...

Embodiment 2

[0084] Determine Pb as follows 2+ 、Ag + and / or Cu 2+ The positions of the corresponding characteristic Raman scattering peaks:

[0085] Prepare a, b, c, d four parts of 2mL 50nmol / L nano-gold mixed solution probes according to the method of Example 1, wherein a does not contain other metal ions, and b contains 350nM Pb 2+ , c contains 350nM Pb 2+ and 400nM Ag + , d contains 350nM Pb 2+ , 400nM Ag + and 250nM Cu 2+ , carry out Raman scattering spectrum detection to 4 samples respectively, obtain the Raman scattering spectrum of each sample, the result is as follows Figure 8 Shown, where the curves a, b, c, d correspond to a, b, c, d4 samples respectively.

[0086] Cross-contrast the Raman scattering spectra of the 4 samples to determine the Pb 2+ 、Ag + and Cu 2+ The corresponding characteristic Raman scattering peaks can be seen at 1615cm -1 for the detection of Pb 2+ The characteristic Raman scattering peak, 365cm -1 to detect Ag + The characteristic Raman scat...

Embodiment 3

[0089] Plot Pb separately 2+ 、Ag + and Cu 2+ The standard relationship curve between the concentration and the peak value of the characteristic Raman scattering peak:

[0090] (1) Draw Pb 2+ The standard relationship curve between the concentration and the peak value of the characteristic Raman scattering peak:

[0091] Adding Pb to the 50nmol / L nano-gold mixed solution probe 2+ , to prepare Pb 2+ Samples with concentrations of 0nM, 100nM, 200nM, 250nM, 300nM, 350nM, 400nM, 450nM, 500nM, 550nM, 600nM and 700nM;

[0092] Perform Raman scattering spectrum detection on each sample to obtain the Raman scattering spectrum of each sample, such as Figure 9 As shown, it is determined from the figure that each sample is at 1615cm -1 The peak of the characteristic Raman scattering peak at ; then with Pb 2+ The concentration is the abscissa, and the sample is at 1615cm -1 The peak of the characteristic Raman scattering peak at is the ordinate, plotting Pb 2+ The standard relat...

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Abstract

The invention relates to an automatic classification detecting method for multiple analytes based on the surface enhancement Raman scattering technology. According to the automatic classification detecting method, a nanogold mixed solution formed by integrating three water-soluble polymers which contain Raman active molecules on the surfaces and are capable of specifically recognizing the target analytes serves as a probe, a water solution containing Pb2+ and a water solution containing Ag+ and a water solution containing Cu2+ are mixed with the nanogold mixed solution respectively or at the same time, detecting is carried out through enhancement of a Raman scattering spectrum, efficient automatic classification selectivity for Pb2+, Ag+ and Cu2+ is achieved through the detection, and therefore the three ions and corresponding contents of the three ions can be identified at the same time. According to the automatic classification detecting method, operation is easy and convenient, sensitivity is high, and wide application prospects are achieved in the fields such as biomedicine and environment monitoring.

Description

technical field [0001] The invention belongs to the field of nanometer applications, in particular to the preparation of an ion detection probe based on surface-enhanced Raman scattering technology, and the self-classification detection of Pb by the probe in aqueous solution 2+ 、Ag + and Cu 2+ and its application. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by heavy metals or their compounds, mainly including mercury, chromium, cadmium, lead, and metalloid arsenic. Among them, lead is a common heavy metal in the ordinary environment and has high toxicity. Since lead cannot be degraded in the environment, it will persist in the environment for a long time, which will inevitably cause toxicity to animals and plants, and directly or indirectly cause harm to human health. Lead is toxic to nerves, blood, digestion and kidneys. After lead in the environment enters the human body through food and breathing, it will cause polar or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 殷俊何亚光史声宇吴云柱
Owner HEFEI UNIV OF TECH
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