Cerebrospinal fluid cell detection method and system based on Raman scattering spectrum

Abstract

The invention discloses a cerebrospinal fluid cell detection method and system based on a Raman scattering spectrum. The method comprises the following steps: 1, preparing a cerebrospinal fluid sample; 2, carrying out single-point test and imaging test on cerebrospinal fluid cells on the sample to obtain original Raman spectrum data; 3, processing the original Raman spectrum data obtained in the step 2 to obtain processed Raman spectrum data, wherein the processing comprises the steps of substrate removal preprocessing and normalization processing; and 4, carrying out data analysis on the Raman spectrum data processed in the step 3 by adopting a deep neural network and principal component analysis, and gathering the spectrums of cerebrospinal fluid cells of the same type together with datanodes of the same color to finish classification and detection. According to the method and the system, the detection and judgment accuracy can be improved, and meanwhile, the report issuing efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical fields of cell detection, principal component analysis (PCA) and deep neural network (DNN), and in particular relates to a method and system for detecting cerebrospinal fluid cells based on Raman scattering spectroscopy. Background technique [0002] At present, cytological detection is the standard method for detecting and counting white blood cells in cerebrospinal fluid. The operation process is to drop the cerebrospinal fluid sample on the pathological grade glass slide for cytocentrifugation, and obtain MGG after staining with May-Grüwald Giemsa (MGG). Stained samples are observed under an optical microscope by cytologists who have been trained for many years, and white blood cells are classified and counted based on cell morphology and staining, combined with years of experience; usually, this process takes 24 hours, which is a long time. In addition, the cytology conclusion obtained from the observation of ...

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Problems solved by technology

[0002]Currently, cytological testing is the standard method for detecting and counting white blood cells in cerebrospinal fluid. After sedimentation, MGG stained samples were obtained after May-Grüwald Giemsa (MGG) staining, and cytology experts who had been trained for many years observed under an optical microscope, and classified and counted white blood cells according to cell morphology and staining, combined with years of experience; Usually, the process takes 24 hours, which takes a long time

In addition, the cytology conclusion obtained from the observation of MGG stained samples is the subjective judgment of cytology experts based on experience, and there is a certain degree of randomness

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