41 results about "Protein Region" patented technology

The invention belongs to the technical field of plant genetic engineering, and discloses a gene for controlling the number of rice grains per spike and application of the gene for controlling the number of rice grains per spike. A nucleotide sequence of OsCKX11 is SEQ ID NO:1 as shown in the description; a protein-coding region nucleotide sequence is SEQ ID NO:2 as shown in the description; and aprotein-coding amino acid sequence is SEQ ID NO:3 as shown in the description. According to the gene for controlling the number of rice grains per spike and application of the gene for controlling thenumber of rice grains per spike, an OsCKX11CRISPR / Cas9 knockout vector is constructed, multiple independent homozygous lines are identified by means of PCR amplification and sequencing, and a mutantwith the rice OsCKX11 gene specifically knocked out to cause rise of a cytokinin level and increase of the number of grains per spike is provided. On the basis of the biological function of increasingthe number of rice grains per spike through function loss of OsCKX11, by means of gene editing, RNA interference, molecular assisted breeding and the like, an existing rice variety can be improved, the number of rice grains per spike can be increased, and a theoretical basis can be provided for breeding of high-yield rice varieties.

A recombinant hybrid virus, including: (a) a deleted adenovirus vector genome comprising the adenovirus 5′ and 3′ cis-elements for viral replication and encapsidation, and further comprising a deletion in an adenovirus genomic region selected from the group consisting of: (i) the polymerase region, wherein said deletion essentially prevents the expression of a functional polymerase protein from said deleted region and said hybrid virus does not otherwise express a functional polymerase protein, (ii) the preterminal protein region, wherein said deletion essentially prevents the expression of a functional preterminal protein from said deleted region, and said hybrid virus does not otherwise express a functional preterminal protein, and (iii) both the regions of (i) and (ii); and (b) a recombinant adeno-associated virus (AAV) vector genome flanked by the adenovirus vector genome sequences of (a), said recombinant AAV vector genome comprising (i) AAV 5′ and 3′ inverted terminal repeats, (ii) an AAV packaging sequence, and (iii) a heterologous nucleic acid sequence, wherein said heterologous nucleic acid sequence is flanked by the 5′ and 3′ AAV inverted terminal repeats of (i). Methods of making and using the recombinant hybrid virus are also disclosed.

A protein construct including a gene encoding a light-sensitive protein fused to at least one of either a low complexity sequence, an intrinsically disordered protein region (IDR), or a repeating sequence of a linker and another gene encoding a light-sensitive protein. Among the many different possibilities contemplated, the protein construct may also advantageously include cleavage tags. This protein construct may be utilized for a variety of functions, including a method for protein purification, which requires introducing the protein construct into a living cell, and inducing the formation of clusters by irradiating the construct with light. The method may also advantageously include cleaving a target protein from an IDR, and separating the clusters via centrifuge. A kit for practicing in vivo aggregation or liquid-liquid phase separation is also included, the kit including the protein construct and a light source capable of producing a wavelength that the light-sensitive protein will respond to.

To ensure maximum cross-strain recognition and reactivity, regions of proteins that are conserved between different Neisserial species, serogroups and strains can be used. The invention provides proteins which comprise stretches of amino acid sequence that are shared across the majority of Neisseria, particularly N. meningitidis and N. gonorrhoeae.

Disclosed is a method for improving plant drought resistance by inhibiting expression of COST1 genes. The nucleotide sequence of the COST1 genes is shown as SEQ ID NO: 1, and the protein sequence coded by the COST1 genes is an amino acid sequence shown as SEQ ID NO: 2. Homologous genes of the COST1 genes comprise tomato SlCOST1 genes, rice OsCOST1 genes, poplar PtCOST1 and PtCOST2 genes, and other genes in other species having a homology degree of more than 40% with fragments in any nucleotide or protein region of COST1.

The invention relates to new coiled-coil domain containing protein 8 (CCDC8) having inhibition on HIV-1 and an application thereof. The CCDC8, which is generated by human cells, in the invention has a strong anti-HIV-1 effect. By means of combination of the CCDC8 with matrix protein region of HIV-1Gag, assembly of the Gag protein on cytomembrane is inhibited. The CCDC8, OBSL1, and E3 ubiquitin ligase Cul7 cause internalization, multi-ubiquitination and degradation of the HIV-1Gag. According to the invention, on the basis of the CCDC8, a new plasmid is established and can be used for express new proteins, namely, the protein composed of 274 amino acids at the N-terminal of the CCDC8, thereby degrading HIV-1.

A rapid, infrared spectroscopic method has been developed to assess the efficacy of targeted chemotherapeutics against the structure of the polypeptide target, based on the effect of natural polymorphic sequence variation on the conformation of the protein. This method has an advantage over the current genomics-based screening, as the new method provides a direct readout of the structural, and hence functional, outcome of polymorphisms to the protein region targeted by drugs. It allows rapid measurement of a protein's susceptibility to therapeutic targeted agents, prior to using the drug as treatment in the patient. This method can be used to identify biomarkers for a response for a protein to a drug which can be readily tested, interpreted, and used in a clinical setting.

The invention provides a recombinant glucose-fructose oxidoreductase and a fungal expression vector as well as a fungal insecticide thereof. A recombinant nucleotide sequence, which comprises a signal peptide sequence for coding the chitinase of beauveria bassiana and a mature protein region sequence for coding the glucose-fructose oxidoreductase of zymonomas mobilis, is constructed by use of the molecular biological technique. According to the recombinant glucose-fructose oxidoreductase, the PgpdA-BbSP-GFOR segment is cloned into a pUC-Bar vector to obtain the fungal expression vector; the fungal expression vector is imported into the wild-type beauveria bassiana Bb0062 strain to obtain the beauveria bassiana transformant of BbSP-GFOR. The recombinant glucose-fructose oxidoreductase effectively blocks the activity of the GNBPs in a host; the time that the fungi knock down insect pests is shortened by 48 hours and the insecticidal efficacy is remarkably improved.