A kind of recombinant glucose-fructose oxidoreductase and its fungal expression vector and fungal insecticide

Abstract

The invention provides a recombinant glucose-fructose oxidoreductase and a fungal expression vector as well as a fungal insecticide thereof. A recombinant nucleotide sequence, which comprises a signal peptide sequence for coding the chitinase of beauveria bassiana and a mature protein region sequence for coding the glucose-fructose oxidoreductase of zymonomas mobilis, is constructed by use of the molecular biological technique. According to the recombinant glucose-fructose oxidoreductase, the PgpdA-BbSP-GFOR segment is cloned into a pUC-Bar vector to obtain the fungal expression vector; the fungal expression vector is imported into the wild-type beauveria bassiana Bb0062 strain to obtain the beauveria bassiana transformant of BbSP-GFOR. The recombinant glucose-fructose oxidoreductase effectively blocks the activity of the GNBPs in a host; the time that the fungi knock down insect pests is shortened by 48 hours and the insecticidal efficacy is remarkably improved.

Description

technical field [0001] The invention belongs to the field of molecular biology, and relates to a recombinant glucose-fructose oxidoreductase and its fungal expression vector and fungal pesticide, in particular to a glucose-fructose oxidoreductase GFOR containing fungal secreted signal peptide and bacteria Sequence recombinant protein and its fungal expression vector and fungal insecticide. Background technique [0002] my country is a large agricultural production country, and the grain output has been harvested year after year, and the use of pesticides has contributed a lot. However, data show that the average amount of pesticides used in my country is 2.5 times the world average. Excessive use of pesticides has polluted the environment and threatened the quality and safety of agricultural products and food safety. Therefore, developing high-efficiency, environmentally safe, and specific biopesticides and reducing the use of conventional chemical pesticides will be benefi...

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Problems solved by technology

However, whether this gene can be introduced into insecticidal fungi to produce gluconolactone during fungal infection, inhibit the immune response of the host, and improve the insecticidal effect of fungi has not yet been reported.

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