The invention relates to primer pairs for fluorescence EMA detection of canine distemper virus (CDV), a kit and a detection method. The primer pairs for fluorescence EMA detection of canine distempervirus (CDV) contain a forward primer, a reverse primer and a probe. The kit comprises the following contents: a redissolved solution, a lyophozyme tube containing primers, a positive quality control and a negative quality control. The detection includes the process of carrying out a normal-temperature nucleic acid amplification technology by using the kit. Through multiple enzymatic reactions of M-MLV reverse transcriptase, a Rnase inhibitor, DNA helicase, single-stranded DNA binding protein, DNA polymerase and the like, RNA to be tested can undergo reverse transcription into cDNA under the condition of constant temperature 37-45 DEG C within 10-30 min, and the cDNA is amplified by millions of times; and and with the cooperation of the fluorescence detection technique, rapid detection of RNA to be tested can be realized. The detection method of the invention has advantages of simple operation, short time and low requirement on equipment, and is suitable for rapid diagnosis of pet infectious diseases.