121 results about "DNA unwinding enzyme" patented technology

The invention discloses an isothermal amplification nucleic acid detection method based on helicase and nicking enzyme. The isothermal amplification nucleic acid detection method comprises the following steps that S1, a specific amplification primer and a Taqman probe with nicking enzyme recognition sites are designed according to a target nucleotide sequence; S2, a sample nucleic acid is subjected to enzyme treatment to obtain a single-chain nucleic acid template; S3, the Taqman probe obtained in the step S1 and the single-chain nucleic acid template obtained in the step S2 are specifically bonded to obtain a specific bonding product, the specific bonding product is digested by using the nicking enzyme, and real-time detection of fluorescence signals is achieved. The helicase and nickingenzyme are used jointly, the Taqman probe with nicking enzyme recognition sites is designed, a large number of nucleic acid products do not need to be amplified, only an appropriate amount of single-chain templates is needed, and then detection can be performed by using the nicking enzyme to constantly cut the probe bound with recognition sites, the shortcoming of lab pollution likely caused by isothermal amplification can be avoided, and the isothermal amplification nucleic acid detection method is simple and quick to operate and suitable for on-site quick detection.