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Method for improving plant drought resistance by inhibiting expression of COST1 genes

A drought resistance and gene technology, applied in the field of genetic engineering, can solve problems such as time-consuming and unclear genetic background, and achieve the effect of improving the drought resistance of plants

Active Publication Date: 2018-12-07
LUDONG UNIVERSITY
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Problems solved by technology

Traditional genetic breeding requires multiple generations such as hybridization and selfing, which is time-consuming and lengthy, and there are problems such as unclear genetic background

Method used

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  • Method for improving plant drought resistance by inhibiting expression of COST1 genes
  • Method for improving plant drought resistance by inhibiting expression of COST1 genes
  • Method for improving plant drought resistance by inhibiting expression of COST1 genes

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Experimental program
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Embodiment 1

[0039] 1. Screening and acquisition of COST1 gene

[0040]Through reverse genetics, a new Arabidopsis DUF641 family gene COST1 (Constitutively Stressed 1) was screened using transcriptome, genome and proteomics and other bioinformatics methods. After sequencing, its nucleotide sequence was as follows: The sequence is shown in SEQ ID NO.1, and its protein sequence is shown in SEQ ID NO.2; protein sequence alignment shows that in addition to COST1, there are three other proteins in Arabidopsis, COST2, COST3 and COST4, which are highly homologous to COST1 ( figure 1 ).

[0041] 2. Homology analysis of COST1 gene and verification of COST1 function

[0042] The T-DNA insertion plant SALK_064001 of COST1 was ordered from the ABRC (Arabidopsis Biological Research Center, www.arabidopsis.org) Arabidopsis mutant library. The insertion position of the T-DNA of this mutant is inside the first and only exon and is homozygous ( figure 2 a, b). The planting and mutant identification m...

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Abstract

The invention discloses a method for improving plant drought resistance by inhibiting expression of COST1 genes. The nucleotide sequence of the COST1 genes is shown as SEQ ID NO:1, and the protein sequence coded by the COST1 genes is an amino acid sequence shown as SEQ ID NO:2. Homologous genes of the COST1 genes include tomato SlCOST1 genes, rice OsCOST1 genes, poplar PtCOST1 and PtCOST2 genes and other genes having the homology degree of more than 40% with fragments in any nucleotide or protein region of COST1 in other species. A foundation is laid for cultivating more drought resistant transgenic plants. The method can be applied to plant genetic improvement.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for improving plant drought resistance by knocking out the COST1 gene or inhibiting the expression of the COST1 gene. Background technique [0002] In today's environment of global warming and extreme shortage of fresh water resources, how to ensure stable food production and feed the world's 7.5 billion people is a great challenge. There is an urgent need to develop new varieties of plants that are more productive and drought-resistant. [0003] In recent years, driven by next-generation sequencing, a large number of plant genome sequences have been published. How to dig deep into the functions of these existing genome sequences and find new functional genes with independent intellectual property rights has become a new challenge. How to use these genes to breed more powerful new crop varieties is imminent. Traditional genetic breeding requires multiple g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00A01H6/00
CPCC07K14/415C12N15/8273
Inventor 包岩宋维萌张洪霞
Owner LUDONG UNIVERSITY
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